Determination of organophosphorus pesticide residues in the liver by gas chromatography with flame photometric detection

• Autorzy: Kiljanek T. , Niewiadowska A. , Semeniuk S.
• Języki publikacji: EN

Abstrakty

EN

A method for simultaneous determination of residues of six organophosphorus pesticides in animal liver samples has been developed and validated. The method is based on the extraction of organophosphorus pesticides with a mixture of acetone-petroleum ether, clean up on a gel permeation chromatography, further clean up on a small silica gel column, and finally determination by gas chromatographx with flame photometric detection. The recovery, investigated by analysing blank liver samples spiked with chlorpyrifos-methyl, chlorpyrifos, diazinon, parathion, and pirimiphos-methyl at levels of 5, 25, 50, and 75 µg/kg, and with chlorfenvinphos at levels of 5, 10, and 15 µg/kg, ranged from 75.6% to 107.5%. Performance characteristics, such as repeatability and within-laboratory reproducibility, expressed as coefficient of variation, were less than 20%, and uncertainty <21%. Linearity ranged between 5 and 1,000 ng/mL. The limits of quantification were 5 µg/kg for all compounds and allowed determination of residues below the MRLs set by the European Union. The satisfacton z-score results of international proficiency tests confirmed good accuracy, reproducibility, and reliability of the developed method.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2011
• Tom: 55
• Numer: 4
• Opis fizyczny: p.731-736,fig.,ref.

Twórcy

autor

Kiljanek T.

• Department of Pharmacology and Toxicology, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Niewiadowska A.

autor

Semeniuk S.

Bibliografia

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