Analysis of immunohistochemical expression of proinflammatory cytokines (IL-1alfa, IL-6, and TNF-alfa) in gallbladder mucosa: comparative study in acute and chronic calculous cholecystitis

• Autorzy: Kasprzak A. , Szmyt M. , Malkowski W. , Przybyszewska W. , Helak-Lapaj C. , Seraszek-Jaros A. , Surdacka A. , Malkowska-Lanzafame A. , Kaczmarek E.
• Języki publikacji: EN

Abstrakty

EN

Background: Several studies have shown increased serum levels of proinflammatory cytokines (IL-1α, IL-6 and TNF-α) in patients with cholelithiasis. The local expression of the proteins involved in pathogenesis of the disease is poorly recognised. Materials and methods: The authors examined immunohistochemically (IHC) the expression status of IL-1α, IL-6, and TNF-α in gallbladder mucosa of the patients with cholelithiasis as related to acute (ACC) and chronic (CCC) types of cholecystitis. Proinflammatory cytokines were quantitatively evaluated in gallbladder mucosa (epithelium and lamina propria) in ACC (n = 16) and CCC (n = 55) groups using modern spatial visualisation technique. Results: Quantitative analysis of IHC signals showed no significant differences in IL-1α and IL-6 immunoexpression in patients with ACC and CCC. A significantly greater IHC expression of TNF-α was detected in CCC as compared with ACC group. In either of the patient groups immunoexpression of IL-1α and of TNF-α was significantly higher than that of IL-6. Immunoexpression of TNF-α was significantly higher than that of IL-1α only in CCC group. A positive correlation was disclosed between IHC expression of IL-1α and body mass index in CCC group. IHC expression of TNF-α correlated positively with expression of CD68 molecule (histiocytic marker), number of leukocytes in blood and higher grading of gallbladder wall in ACC group. Conclusions: A more pronounced IHC expression of TNF-α and IL-1α than IL-6 in both types of cholecystitis may suggest the role of these cytokines in pathogenesis of cholelithiasis. IHC expression of TNF-α shows better correlation with clinical/laboratory data in acute cholecystitis, and its quantitative prevalence over the remaining cytokines points to the role of the TNF-α in maintenance of inflammation in the course of cholelithiasis. (Folia Morphol 2015; 74, 1: 65–72)

• Wydawca: -
• Czasopismo: Folia Morphologica
• Rocznik: 2015
• Tom: 74
• Numer: 1
• Opis fizyczny: p.65-72,fig.,ref.

Twórcy

autor

Kasprzak A.

• Chair and Department of Histology and Embryology, Poznan University of Medical Sciences, Poznan, Poland

autor

Szmyt M.

• Chair and Department of Histology and Embryology, Poznan University of Medical Sciences, Poznan, Poland

autor

Malkowski W.

• Chair of General Surgery T.Chalubinski Municipal Hospital in Ostrow Wielkopolski, Ostrow Wielkopolski, Poland

autor

Przybyszewska W.

• Chair and Department of Histology and Embryology, Poznan University of Medical Sciences, Swiecickiego 6, 60–781 Poznan, Poland

autor

Helak-Lapaj C.

• Chair of Clinical Pathomorphology, Department of Bioinformatics and Computational Biology, Poznan University of Medical Sciences, Poznan, Poland

autor

Seraszek-Jaros A.

• Chair of Clinical Pathomorphology, Department of Bioinformatics and Computational Biology, Poznan University of Medical Sciences, Poznan, Poland

autor

Surdacka A.

• Department of Conservative Dentistry and Periodontology, Poznan University of Medical Sciences, Poznan, Poland

autor

Malkowska-Lanzafame A.

• Department of Conservative Dentistry and Periodontology, Poznan University of Medical Sciences, Poznan, Poland

autor

Kaczmarek E.

• Chair of Clinical Pathomorphology, Department of Bioinformatics and Computational Biology, Poznan University of Medical Sciences, Poznan, Poland

Bibliografia

• 1. Arend WP, Massoni RJ (1986) Characteristics of bacterial lipopolysaccharide induction of interleukin 1 synthesis and secretion by human monocytes. Clin Exp Immunol, 64: 656–664.
• 2. Dinarello CA (2006) Interleukin 1 and interleukin 18 as mediators of infammation and the aging process. Am J Clin Nutr, 83: 447S–455S.
• 3. Gilloteaux J, Tomasello LM, Elgison DA (2003) Lipid deposits and lipo-mucosomes in human cholecystitis and epithelial metaplasia in chronic cholecystitis. Ultrastruct Pathol, 27: 313–321.
• 4. Hotamisligil GS, Shargill NS, Speigelman BM (1993) Adipose expression of tumor necrosis factor-alpha: direct role in obesity-linked insulin resistance. Science, 259: 87–91.
• 5. Hsu SM, Raine L, Fanger H (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem, 29: 577–580.
• 6. Kaczmarek E, Strzelczyk R (2005) From two to three-dimensional visualisation of structures in light and confocal microscopy — applications for biomedical studies. In: Mendez-Vilas A, Labajos-Broncano L eds. Current issues on multidisciplinary microscopy research and education. FORMATEX microscopy book series no. II Formatex Research Centre, Badajoz, pp. 289–295.
• 7. Kasprzak A, Malkowski W, Helak-Łapaj C, Seraszek A, Kaczmarek E, Adamek A, Zabel M (2010) Polysaccharides and mucin 5AC (MUC5AC) expression in gallbladder mucosa of young patients with gallstones as evaluated by spatial visualization and quantification. Folia Histochem Cytobiol, 48: 646–657.
• 8. Kishimoto T (1989) The biology of interleukin-6. Blood, 74: 1–10.
• 9. Kishimoto T (2010) IL-6: from its discovery to clinical applications. Int Immunol, 22: 347–352.
• 10. Kitasato A, Tajima Y, Kuroki T, Tsutsumi R, Adachi T, Mishima T, Kanematsu T (2007) Inflammatory cytokines promote inducible nitric oxide synthase-mediated DNA damage in hamster gallbladder epithelial cells. World J Gastroenterol, 13: 6379–6384.
• 11. Marsch-Ziegler U, Palme G (1982) Effect of a lithogenic diet and starvation on the regeneration of gallbladder epithelium. Leber Magen Darm, 12: 157–161.
• 12. Mathur A, Al-Azzawi HH, Lu D, Yancey KW, Swartz-Basile DA, Nakeeb A, Pitt HA (2008) Steatocholecystitis: the influence of obesity and dietary carbohydrates. J Surg Res, 147: 290–297.
• 13. Pitt HA (2007) Hepato-pancreato-biliary fat: the good, the bad and the ugly. HPB (Oxford), 9: 92–97.
• 14. Prystowsky JB, Rege RV (1997) Interleukin-1 mediates guinea pig gallbladder inflammation in vivo. J Surg Res, 71: 123–126.
• 15. Rege RV (2000) Inflammatory cytokines alter human gallbladder epithelial cell absorption/secretion. J Gastrointest Surg, 4: 185–192.
• 16. Rege RV, Prystowsky JB (1996) Inflammatory properties of bile from dogs with pigment gallstones. Am J Surg, 171: 197–201.
• 17. Rosai J (2004) Gallbladder and extrahepatic bile ducts. In: Rosai J ed. Rosai and Ackerman’s Surgical Pathology, Mosby, Edinburgh, London, New York, Oxford, Philadelphia, St. Louis, Sydney, Toronto, pp. 1035–1055.
• 18. Rose-John S, Scheller J, Elson G, Jones SA (2006) Interleukin-6 biology is coordinated by membrane-bound and soluble receptors: role in inflammation and cancer. J Leukoc Biol, 80: 227–236.
• 19. Savard CE, Blinman TA, Choi HS, Lee SK, Pandol SJ, Lee SP (2002) Expression of cytokine and chemokine mRNA and secretion of tumor necrosis factor-alpha by gallbladder epithelial cells: response to bacterial lipopolysaccharides. BMC Gastroenterol 2: 23 (doi: 10.1186/1471-230X-2-23).
• 20. Schirmer BD, Winters KL, Edlich RF (2005) Cholelithiasis and cholecystitis. J Long Term Eff Med Implants, 15: 329–338.
• 21. Sehgal PB (1996) Interleukin-6-type cytokines in vivo: regulated bioavailability. Proc Soc Exp Biol Med, 213: 238–247.
• 22. Tazuma S, Kajiyama G (2001) Carcinogenesis of malignant lesions of the gallbladder. The impact of chronic inflammation and gallstones. Langenbecks Arch Surg, 386: 224–229.
• 23. Tsai CJ (2009) Steatocholecystitis and fatty gallbladder disease. Dig Dis Sci, 54: 1857–1863.
• 24. Tsaregorodtseva TM, Zotina MM, Serova TI, Sokolova GN, Iakimchuk GN (2003) Interleukins in chronic diseases of digestive organs. Ter Arkh, 75: 7–9.
• 25. Yasoshima M, Kono N, Sugawara H, Katayanagi K, Harada K, Nakanuma Y (1998) Increased expression of interleukin-6 and tumor necrosis factor-alpha in pathologic biliary epithelial cells. Lab Invest, 78: 89–100.

Identyfikatory

DOI

10.5603/FM.2015.0011