PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2011 | 67 | 07 |

Tytuł artykułu

Apoptotic index and histological assessment of hepatocytes after adriamycin treatment

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Adriamycin is an antineoplastic antibiotic which is biodegragated mainly in the liver. The purpose of this study was to analyse the apoptotic index and the histological structure of apoptotic cells in the rat liver. Rats were examined 4 and 7 weeks after adriamycin treatment. The animals were divided into four equal groups: groups I and III, consisting of rats treated with a single intraperitoneal dose of adriamycin (5 mg/kg b.w) and decapitated after 4 or 7 weeks; groups II and IV comprising control rats treated with a single intraperitoneal dose of 0.9% NaCl (0.5 ml) and decapitated after 4 or 7 weeks. After decapitation, liver specimens were collected and embedded in paraffin. Slides were stained with haematoxylin and eosin. The degree of apoptosis was determined quantitatively using the apoptotic index. Statistical analysis was performed using the one-way ANOVA test and Student’s t-test. A single dose of adriamycin induced apoptosis of hepatocytes, which increased with time The apoptotic index was significantly higher in the group examined 7 weeks after adriamycin administration. The histological structure of the liver in the experimental groups was similar. The lesions observed were focal. The blurring of the hepatic cell membrane and a focal disintegration of architectonics concerning the shape and size of hepatocytes were visible. Chromatin in the nuclei was dispersed. Some nuclei showed peripheral chromatin condensation. The cytoplasm of some cells showed numerous vacuoles. Naked nuclei and congestion resulting from vessel damage were visible.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

67

Numer

07

Opis fizyczny

p.467-469,fig.,ref.

Twórcy

  • Department of Animal Anatomy and Histology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-950 Lublin
autor
autor
autor

Bibliografia

  • 1.Bagnato A., Bianchi C., Caputo A., Silvestrini B., Florich A.: Enhancing effect of loniamide on the inhibition of mitochondrial respiration by adriamycin. Anticancer Res. 1987, 7, 799-802.
  • 2.Bianchi C., Bagnato A., Paggi M. G., Floridi A.: Effect of adriamycin on electron transport in rat heart, liver, and tumor mitochondria. Exp. Mol. Pathol. 1987, 46, 123-135.
  • 3.Drzewoski J., Robak T.: Clinical pharmacology of antineoplastic drugs. Ed. Nauka, Warszawa 1991.
  • 4.Eom Y. W., Kim M. A., Park S. S., Goo M. J., Kwon H. J., Sohn S., Kim W. H., Yoon G., Choi K. S.: Two distinct modes of cell death induced by doxorubicin: apoptosis and cell death through mitotic catastrophe accompanied by senescence-like phenotype. Oncogene 2005, 24, 4765-4777.
  • 5.Fizames C.: Models of preclinical studies of anthracyclines. Pathol. Biol. Paris 1987, 35, 41-48.
  • 6.Hirata K., Kaneko A., Ogawa K., Hayasake H., Ohoe T.: Effect of endotoxin on rat liver. Analysis of acid phosphatase izozymes in the liver of normal and endotoxin-treated rats. Lab. Invest. 1980, 43, 165-171.
  • 7.Matusewicz W., Robak T.: Anthracyclines. Pol. Arch. Med. Wewn. 1985, 73, 242-249.
  • 8.Mendocha J.: Localization of the cell death types in hepatic acinus depending of the time and dose of adriamycin. Doctoral Thesis, Ed. Medical University, Lublin, Poland 2009.
  • 9.Muraoka S., Miura T.: Thiol oxidation induced by oxidative action of adriamycin. Free Radic. Res. 2004, 38, 963-968.
  • 10.Oz E., Erbas D., Surucu H. S., Duzgun E.: Prevention of doxorubicin-induced cardiotoxicity by melatonin. Mol. Cell. Biochem. 2006, 282, 31-37.
  • 11.Papierkowski A., Jabłoński M., Skomra D., Pasternak K.: The influence of natrium fluoride on serum protein and cholesterol levels in rats with adriblastin-induced nephritic syndrome. Ann. Univ. Mariae Curie Sklodowska 1999, 54, 19-23.
  • 12.Park S. S., Eom Y. W., Choi K. S.: Cdc2 and Cdk2 play critical roles in low dose doxorubicin-induced cell death through mitotic catastrophe but not in high dose doxorubicin-induced apoptosis. Biochem. Biophys. Res. Commun. 2005, 334, 1014-1021.
  • 13.Pedrycz A., Boratyński Z., Drelich G.: Apoptotic index and histological assessment of renal tubular epithelial cells during anthracycline-induced apoptosis. Influence of time. Bull. Vet. Inst. Pulawy 2010, 54, 55-58.
  • 14.Pedrycz A., Boratyński Z., Krasowski A.: Pathomorphology of hepatocytes of rats treated with adriamycin. Bull. Vet. Inst. Pulawy 2010, 54, 641-644.
  • 15.Pedrycz A., Kosiecz D., Drelich G., Ossowska B.: Over-expression of biomarkers of environmental stress in renal epithelial cells resulting from proapoptotic activity of adriamycin: An immunohistochemical assessment. Curr. Probl. Psychiatry 2010, 11, 161-165.
  • 16.Skomra D., Chibowski D.: Assessment of Daunorubicin nephrotoxicity and effect of tocopherol and acsorbic acid on lesions induced in rat's kidneys. Part I. Histological, histochemical and biochemical studies. Ann. Univ. Mariae Curie Sklodowska, sectio D, 1993, 98, 181-189.
  • 17.Speth P. A., Van Hoesel Q. G., Haanen C.: Clinical pharmacokinetics of doxorubicin. Clin. Pharmacokinet. 1988, 15, 15-31.
  • 18.Zeidan Q., Strauss M., Porras N., Anselmi G.: Differential long-term subcellular responses in heart and liver to adriamycin stress. Exogenous L-carnitine cardiac and hepatic protection. J. Submicrosc. Cytol. Pathol. 2002, 34, 315-321.
  • 19.Zima T., Tesar V., Crkovska J., Stejskalova A., Platenik J., Teminova J., Nemecek K., Janebova M., Stipek S.: ICRF-187 (dexrazoxan) protects from adriamycin-induced nephrotic syndrome in rats. Nephrol. Dial. Transplant. 1998, 13, 1975-1979.

Uwagi

Rekord w opracowaniu

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-61a95197-899e-4dda-817f-801db55c9c7f
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.