Voltage-gated calcium chanels affected acetyl-CoA metabolism in cholinergic neurons exposed on Zn ions

• Autorzy: Zysk M. , Gapys B. , Gul-Hinc S. , Szutowicz A. , Bielarczyk H.
• Języki publikacji: EN

Abstrakty

EN

BACKGROUND AND AIMS: Cholinergic neurons produce acetylCoA, which is subsequently used as a fuel for energy production. Furthermore, exclusively those neurons produce acetylcholine from acetyl-CoA. As a results, extra utilization pathway may induce acetyl-CoA shortages and consequently impairment of brain energy metabolism. Disturbances in Ca-signaling could play regulatory role in neurons susceptibility to neurodegenerative conditions. The aim of our study was to investigate whether the Voltage-Gated Calcium Channels (VGCCs) could moderate the cholinergic neurons susceptibility on neurodegeneration. METHODS: Selected blockers of VGCCs (10 µM nifedipine, 0.2 µM ω-conotoxin-MVIIC, 0.5 µM ω-conotoxin-GVIA) were used as a Ca-depletion factors in SN56 neuroblastoma cells. RESULTS: Short-term SN56 cells exposition on 0.15 mM Zn increased the Zn level from 0.6 to 36 nmol/mg protein. However, in the presence of 10 µM nifedipine and ω-conotoxins, the Zn-accumulation were decreased by about 50%. Zn caused in SN56 about 49% increase of nonviable cells fraction. Whereas incubation cells with VGCCs blockers and Zn, led to 25% decline in the number of trypan blue positive cells the acetyl-CoA level in SN56 was 26.9 pmol/mg protein. However, the SN56 cells exposition on 0.15mM Zn decreased its level by 43%. In addition, acetyl-CoA level in VGCCs-blocked SN56 was as high as in control conditions. CONCLUSIONS: Achieved results indicated that VGCCs regulated the Zn-evoked neurotoxic effects on acetyl-CoA metabolism in SN56 cholinergic cells. Moreover, VGCCs might play particular role in neurotoxicity of Zn and show that disturbance of Ca homeostasis in this condition can be one of the factors which moderate acetyl-CoA metabolism in cholinergic neurons. Supported by MN0059/08 and ST-57 GUMed fund.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2015
• Tom: 75
• Numer: Supl.
• Opis fizyczny: p.S55

Twórcy

autor

Zysk M.

• Chair of Clinical Biochemistry, Department of Laboratory Medicine, Medical University of Gdansk, Gdansk, Poland

autor

Gapys B.

• Chair of Clinical Biochemistry, Department of Laboratory Medicine, Medical University of Gdansk, Gdansk, Poland

autor

Gul-Hinc S.

• Chair of Clinical Biochemistry, Department of Laboratory Medicine, Medical University of Gdansk, Gdansk, Poland

autor

Szutowicz A.

• Chair of Clinical Biochemistry, Department of Laboratory Medicine, Medical University of Gdansk, Gdansk, Poland

autor

Bielarczyk H.

• Chair of Clinical Biochemistry, Department of Laboratory Medicine, Medical University of Gdansk, Gdansk, Poland