Proteolysis on the body surface of pyrethroid-sensitive and resistant Varroa destructor

• Autorzy: Strachecka A. , Borsuk G. , Olszewski K. , Paleolog J. , Lipinski Z.
• Języki publikacji: EN

Abstrakty

EN

The aim of this work was to determine the activity of proteases and protease inhibitors sampled from the body surface of tau-fluvalinate-sensitive and resistant V. destructor. Proteins were isolated from the tau-fluvalinate-sensitive and resistant mites, while mites untreated with tau-fluvalinate constituted the control. Subsequently, the following methodology was applied: protein concentration assay by the Lowry method — as modified by Schacterle and Pollack; assay of proteolytic activity in relation to various substrates (gelatine, haemoglobin, ovoalbumin, albumin, cytochrome C, casein) by the modified Anson method; identification of proteolytic activity in relation to diagnostic inhibitors of proteolytic enzymes (pepstatin A, PMSF, iodoacetamide, o-phenantrolin), using the Lee and Lin method; identification of acidic, neutral and basic protease activities by means of the modified Anson method; electrophoretic analysis of proteins in a polyacrylamide gel for protease detection with the Laemmli method and for protease inhibitor detection with the Felicioli method. The highest value of protein concentration was found in the tau-fluvalinate-sensitive V. destructor, while the highest activity levels of acidic, neutral and alkaline proteases were observed in the tau-fluvalinate-resistant mites. Aspartic, serine, thiolic and metallic proteases were found in the drug-resistant and drug-sensitive Varroa mites. The control samples were found to contain aspartic and serine proteases. In an acidic and alkaline environment, the results revealed a complete loss of inhibitor activities in the in vitro analyses and electrophoresis. Serine protease inhibitor activities (at pH 7.0) were high, especially in the group of tau-fluvalinate-resistant mites.

Słowa kluczowe

EN

proteolytic system; body surface; pyrethroid; Varroa destructor; protein; inhibitory activity; serine protease inhibitor; tau-fluvalinate-resistant mite

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 2013
• Tom: 58
• Numer: 1
• Opis fizyczny: p.64-69,fig.,ref.

Twórcy

autor

Strachecka A.

• Department of Biological Basis of Animal Production, Faculty of Biology and Animal Breeding, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland

autor

Borsuk G.

autor

Olszewski K.

autor

Paleolog J.

autor

Lipinski Z.

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