Epilithic biofilms in Lake Baikal: screening and diversity of PKS and NRPS genes in the genomes of heterotrophic bacteria

• Autorzy: Sukhanova E. , Zimens E. , Kaluzhnaya O. , Parfenova V. , Belykh O.
• Języki publikacji: EN

Abstrakty

EN

A collection of heterotrophic bacteria consisting of 167 strains was obtained from microbial communities of biofilms formed on solid substrates in the littoral zone of Lake Baikal. Based on the analysis of 16S rRNA gene fragments, the isolates were classified to four phyla: Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes. To assess their biotechnological potential, bacteria were screened for the presence of PKS (polyketide synthase) and NRPS (non-ribosomal peptide synthetases) genes. PKS genes were detected in 41 strains (25%) and NRPS genes in 73 (43%) strains by PCR analysis. The occurrence of PKS genes in members of the phylum Firmicutes (the genera Bacillus and Paenibacillus) was 34% and NRPS genes were found in 78%. In Proteobacteria, PKS and NRPS genes were found in 20% and 32%, and in 22% and 22% of Actinobacteria, respectively. For further analysis of PKS and NRPS genes, six Bacillus and Paenibacillus strains with antagonistic activity were selected and underwent phylogenetic analysis of 16S rRNA genes. The identification of PKS and NRPS genes in the strains investigated was demonstrated among the homologues the genes involved in the biosynthesis of antibiotics (bacillaene, difficidine, erythromycin, bacitracin, tridecaptin, and fusaricidin), biosurfactants (iturin, bacillomycin, plipastatin, fengycin, and surfactin) and antitumor agents (epothilone, calyculin, and briostatin). Bacillus spp. 9А and 2А strains showed the highest diversity of PKS and NRPS genes. Bacillus and Paenibacillus strains isolated from epilithic biofilms in Lake Baikal are potential producers of antimicrobial compounds and may be of practical interest for biotechnological purposes.

Słowa kluczowe

EN

epilithic biofilm; Lake Baikal; screening; diversity; PKS gene; NRPS gene; genome; heterotrophic bacteria; secondary metabolite; bacteria

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2018
• Tom: 67
• Numer: 4
• Opis fizyczny: p.501-516,fig.,ref.

Twórcy

autor

Sukhanova E.

• Limnological Institute, Siberian Branch of Russian Academy of Sciences, Irkutsk, Russia

autor

Zimens E.

• Limnological Institute, Siberian Branch of Russian Academy of Sciences, Irkutsk, Russia

autor

Kaluzhnaya O.

• Limnological Institute, Siberian Branch of Russian Academy of Sciences, Irkutsk, Russia

autor

Parfenova V.

• Limnological Institute, Siberian Branch of Russian Academy of Sciences, Irkutsk, Russia

autor

Belykh O.

• Limnological Institute, Siberian Branch of Russian Academy of Sciences, Irkutsk, Russia

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Identyfikatory

DOI

10.21307/pjm-2018-060