Study of chemical coding of the CSMG neurons supplying prepyloric region of the porcine stomach following acetylsalicylic acid supplementation and after partial stomach resection

• Autorzy: Calka J. , Palus K.
• Języki publikacji: EN

Abstrakty

EN

BACKGROUND AND AIMS: The present study was designed to define localization and chemical coding of the sympathetic perikarya innervating the porcine stomach prepyloric area in physiological state, following prolonged aspirin supplementation and after partial stomach resection. METHODS: The study was performed on 3 groups of 5 immature female pigs. The neuronal retrograde marker Fast Blue (FB) was injected into the anterior prepyloric wall of the stomach of control, acetylsalicylic acid treated (ASA) and partial stomach resection (RES) groups. Animals in ASA group were given acetylsalicylic acid orally for 21 days. On 22nd day after injection, in animals of RES group partial stomach resection was performed. On 28th day all pigs were euthanized. Then cryostat sections were double immunolabeled using primary antisera directed towards tyrosine hydroxylase (TH), dopamine β-hydroxylase (DβH), neuropeptide Y (NPY), galanin (GAL), somatostatin (SOM), nitric oxide synthase (NOS), Leu 5-Enkephalin (LENK). As the secondary antibody AlexaFluor 546 and AlexaFluor 488 were applied. In the coeliacsuperior mesenteric ganglion (CSMG) 1615±20.73 FB+ (gastric) neurons have been identified. RESULTS: In the control group, gastric neurons expressed TH (94.85±1.01%), DβH (97.10±0.97%), NPY (46.88±2.53%), SOM (14.97±1.57%) and GAL (8.40±0.53%). In ASA and RES groups, TH- and DβH- positive nerve cells were reduced (ASA: 81.78±0.87% and 86.42±0.94%; RES: 84.56±2.56% and 88.30±1.62%). Moreover, in both groups increased expression of NPY (ASA: 76.59±3.02%, RES: 64.93±2.32%), SOM (ASA: 33.72±4.39%, RES: 39.02±3.65%), GAL (ASA: 26.45±2.75%, RES: 31.13±1.64%) as well as de novo synthesis of NOS (ASA: 6.13±1.11%, RES: 7.29±0.65%) and LENK (ASA: 4.77±0.42%, RES: 3.92±0.45%) in traced CSMG neurons were observed. CONCLUSIONS: Our results indicate involvement of studied substances in the development and presumably counteraction of gastric inflammation and survival of damaged neurons.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2015
• Tom: 75
• Numer: Supl.
• Opis fizyczny: p.44-45

Twórcy

autor

Calka J.

• Department of Clinical Physiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland

autor

Palus K.

• Department of Clinical Physiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland