Differential responses of pea seedlings to indole acetic acid under manganese toxicity

• Autorzy: Gangwar S. , Singh V. P. , Prasad S. M. , Maurya J. N.
• Języki publikacji: EN

Abstrakty

EN

Present study showed the responses of pea seedlings to exogenous indole acetic acid (IAA; 10 and 100 µM) application under manganese (Mn; 50, 100 and 250 µM) toxicity. Manganese and 100 µM IAA alone as well as in combination decreased growth of pea seedlings compared to control. Moreover, some parameters of oxidative stress—hydrogen peroxide (H₂O₂) and malondialdehyde (MDA) were also increased by single and combined treatments of Mn and 100 µM IAA compared to control. In contrast, addition of 10 µM IAA together with Mn, alleviated Mn toxicity symptoms and promoted growth led to the decrease in H₂O₂ and MDA levels compared to Mn treatments alone. Under single and combined treatments of Mn and 100 µM IAA, catalase activity decreased while superoxide dismutase and ascorbate peroxidase activities increased and glutathione reductase and dehydroascorbate reductase exhibited differential responses. However, addition of 10 µM IAA together with Mn, increased activities of studied enzymatic antioxidants. Root and shoot reduced ascorbate (AA) and reduced glutathione (GSH) and, their reduced/oxidized ratios decreased while dehydroascorbate (DHA) and oxidized glutathione (GSSG) contents increased compared to control following single and combined treatments of Mn and 100 µM IAA. However, supply of 10 µM IAA together with Mn, increased AA and GSH, and their reduced/oxidized ratios in root and shoot compared to Mn treatments alone. This study thus suggests that 10 µM of IAA was able to increase Mn tolerance in pea seedlings under Mn toxicity while opposite was noticed for 100 µM IAA.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2011
• Tom: 33
• Numer: 2
• Opis fizyczny: p.451-462,fig.,ref.

Twórcy

autor

Gangwar S.

• Department of Plant Science, M.J.P. Rohilkhand University, Bareilly 243006, India

autor

Singh V. P.

• Ranjan Plant Physiology and Biochemistry Laboratory, Department of Botany, University of Allahabad, Allahabad 211002, India

autor

Prasad S. M.

• Ranjan Plant Physiology and Biochemistry Laboratory, Department of Botany, University of Allahabad, Allahabad 211002, India

autor

Maurya J. N.

• Department of Plant Science, M.J.P. Rohilkhand University, Bareilly 243006, India

Bibliografia

• Aebi II (1984) Catalase in vitro. Methods Enzymol 105:121–126
• Ali B, Rani I, Hayat S, Ahmad A (2007) Effect of 4-Cl-indole acetic acid on seed germination of Cicer arietinum exposed to cadmium. Acta Bot Croat 66:57–65
• Arditti J, Dunn A (1969) Environmental plant physiology—experiments in cellular and plant physiology. Holt, Rinehart and Winston Inc., New York
• Bowler C, Montagu MV, Inzé D (1992) Superoxide dismutase and stress tolerance. Annu Rev Plant Physiol Plant Mol Biol 43:83–116
• Brehe JE, Burch HB (1976) Enzymatic assay for glutathione. Anal Biochem 74:189–197
• Buettner GR, Jurkiewiez BA (1996) Chemistry and biochemistry of ascorbic acid. In: Cadenas E, Packer L (eds) Handbook of antioxidants. Marcel Dekker, New York, pp 91–115
• Chakrabarti N, Mukherji S (2003) Effect of phytohormone pretreatment on nitrogen metabolism in Vigna radiata under salt stress. Biol Plant 46:63–66
• de Melo MP, de Lima TM, Pithon-Curi TC, Curi R (2004) The mechanism of indole acetic acid cytotoxicity. Toxicol Lett 148:103–111
• Egamberdieva D (2009) Alleviation of salt stress by plant growth regulators and IAA producing bacteria in wheat. Acta Physiol Plant 31:861–864
• Fecht-Christoffers MM, Maier P, Horst WJ (2003) Apoplastic peroxidases and ascorbate are involved in manganese toxicity and tolerance of Vigna unguiculata. Physiol Plant 117:237–244
• Giannopolitis CN, Reis SK (1977) Superoxide dismutase. I. Occurrence in higher plants. Plant Physiol 59:309–314
• González A, Steffen KL, Lynch JP (1998) Light and excess manganese: implications for oxidative stress in common bean. Plant Physiol 118:493–504
• Gossett DR, Millhollon EP, Cran LM (1994) Antioxidant response to NaCl stress in salt-sensitive cultivars of cotton. Crop Sci 34:706–714
• Grill E, Winnacker EL, Zenk MH (1985) Phytochelatins: the principal heavy-metal complexing peptides of higher plants. Science 230:674–676
• Hodges DM, DeLong JM, Forney CF, Prange RK (1999) Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta 207:604–611
• Joo JH, Yoo HJ, Hwang I, Lee JS, Nam KH, Bae YS (2005) Auxin induced reactive oxygen species production requires the activation of phosphotidylinositol 3-kinase. FEBS Lett 579:1243–1248
• Krantev A, Yordanova R, Janda T, Szalai G, Popova L (2008) Treatment with salicylic acid decrease the effect of cadmium on photosynthesis in maize plants. J Plant Physiol 165:920–931
• Lamoureux GL, Rusness DG (1993) Glutathione in the metabolism and detoxification of xenobiotics in plants. In: de Kok LJ, Stulen I, Rennenberg H, Brunold C, Rauser WE (eds) In sulfur nutrition and assimilation in higher plants. SPB Academy, The Hague, pp 221–237
• Lidon FC, Henriques FS (1993) Oxygen metabolism in higher plant chloroplasts. Photosynthetica 29:249–279
• Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275
• Marschner H (1995) Mineral nutrition of higher plants. Academic Press, London
• May MJ, Vernoux T, Leaver C, Van Montagu M, Inzé D (1998) Glutathione homeostasis in plants: implications for environmental sensing and plant development. J Exp Bot 49:649–667
• Mittler R (2002) Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci 7:405–410
• Montillet JL, Chamnongpol S, Rustérucci C, Dat J, Van de Cotte B, Agnel JP, Battesti C, Inzé D, Van Breusegen F, Triantsphylides C (2005) Fatty acid hydroperoxides and H₂O₂ in the hypersensitive cell death in tobacco leaves. Plant Physiol 138:1516–1526
• Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880
• Noctor G, Foyer CH (1998) Ascorbate and glutathione: keeping active oxygen under control. Annu Rev Plant Physiol Plant Mol Biol 49:249–279
• Padh H (1990) Cellular functions of ascorbic acid. Biochem Cell Biol 68:1166–1173
• Salin ML (1998) Toxic oxygen species and protective systems of the chloroplast. Physiol Plant 72:681–689
• Sánchez M, Queijeiro E, Revilla G, Zara I (1997) Changes in ascorbic acid levels in apoplastic fluid during growth of pine hypocotyls. Effects on peroxidase activities associated with cell walls. Physiol Plant 101:815–820
• Satyanarayana YV, Saraf R (2007) Iron and manganese contamination: sources, adverse effects and control methods. J Environ Sci Eng 49:333–336
• Schaedle M, Bassham JA (1977) Chloroplast glutathione reductase. Plant Physiol 59:1011–1012
• Shi Q, Zhu Z (2008) Effect of exogenous salicylic acid on manganese toxicity, element contents and antioxidative system in cucumber. Environ Exp Bot 63:317–326
• Shi Q, Zhu Z, Xu M, Qian Q, Yu J (2006) Effect of excess manganese on the antioxidant system in Cucumis sativus L. under two light intensities. Environ Exp Bot 58:197–205
• Velikova V, Yordanov I, Edreva A (2000) Oxidative stress and some antioxidant system in acid rain-treated bean plants. Plant Sci 151:59–66
• Wang H, Shan X, Wen B, Owens G, Fang J, Zhang S (2007) Effect of indole acetic acid on lead accumulation in maize (Zea mays L.) seedlings and relevant antioxidant response. Environ Exp Bot 61:246–253
• Yannarelli GG, Noriega GO, Batlle A, Tomaro ML (2006) Heme oxygenase up-regulation in ultraviolet-B irradiated soybean plants involves reactive oxygen species. Planta 224:1154–1162

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