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2015 | 71 | 04 |

Tytuł artykułu

Wpływ lewamizolu i trichlorfonu na zwalczanie kapilariozy przewodu pokarmowego danio pręgowanego

Warianty tytułu

EN
Efect of levamisole and trichlorfon on the control of intestinal capilariasis in zebrafish

Języki publikacji

PL

Abstrakty

EN
The aim of the present study was to analyze the effectiveness of levamisole and trichlorphon in controlling intestinal capilariasis in zebrafish. Various protocols of treatment against this parasite were successively tested. Fish (280 g ± 53 g) were randomly assigned to 3 experiments. In experiment I, fish (n = 160) were administered levamisole at 1 mg/L (group 1), 2 mg/L (group 2), 5 mg/L (group 3), and 10 mg/L (group 4), by 72 h bath. In experiment II, fish (n = 160) were administered levamisole by bath (as in experiment I) and per os at 200 mg/100g diet twice a day for 3 days. In experiment III, fish (n = 160) were administered levamisole by bath (as in experiment I) and trichlorphon per os at 0.2 mg/100 g diet twice a day for 3 days. These schemes of treatment were repeated at days 7 and 14. At the beginning of treatment,100% of fish were infested by Capillariinae. The mean abundance was estimated at 3.5 (range of intensity: 2-5). In experiments I and II, 21 days after the beginning of treatment, fish were still infested by live parasites. However, with the highest dose of levamisole, the mean abundance of invasion decreased to 0.8 (range of intensity: 0-3) in experiment I, and to 0.3 (range of intensity: 0-2) in experiment II – a reduction of 73.33% and 90.00%, respectively. The combined treatment in experiment III (levamisole by bath and trichlorphon per os) was the most effective. Only in experiment III were parasites eliminated after 14 days in groups 2 and 3, and after 21 days in groups 1, 2, 3 and 4. These results suggest that a combination of levamisole and trichlorphon could be an effective alternative for the control of nematode zebrafish infections, including those by levamisole-resistant strains.

Wydawca

-

Rocznik

Tom

71

Numer

04

Opis fizyczny

s.245-250,fot.,rys.,tab.,bibliogr.

Twórcy

autor
  • Zakład Chorób Ryb i Biologii, Instytut Biologicznych Podstaw Chorób Zwierzat, Wydział Medycyny Weterynaryjnej, Uniwersytet Przyrodniczy w Lublinie, ul.Akademicka 12, 20-033 Lublin
  • Zakład Parazytologii i Chorób Inwazyjnych, Instytut Biologicznych Podstaw Chorób Zwierząt, Wydział Medycyny Weterynaryjnej, Uniwersytet Przyrodniczy w Lublinie, ul.Akademicka 12, 20-033 Lublin

Bibliografia

  • 1. Anderson R. C.: Nematode parasites of vertebrates their development and transmission. CABI Publishing CAB, Wallingford, Oxon (UK) 2000, 605-609.
  • 2. Brandal P. L., Egidius E.: Preliminary report on oral treatment against salmon lice, Lepeophtheirus salmonis, with Neguvon. Aquaculture 1997, 10, 177-178.
  • 3. Geets A., Liewes E. W., Ollevier F.: Efficacy of some anthelmintics against the swimbladder nematode Anguillicola crassus of eel Anguilla anguilla undersaltwater conditions. Dis. Aquat. Org. 1992, 13, 123-128.
  • 4. Iglesias R., Centeno L., García N., García-Estévez J. M.: Pseudocapillaria (Pseudocapillaria) moraveci sp. n. (Nematoda: Capillariidae) from the stomachof Gobius paganellus (Perciformes: Gobiidae) from Vigo estuary (NW Spain).Folia Parasitol. 2013, 60, 135-140.
  • 5. Kent M. L., Bishop-Stewart J. K., Matthews J. L., Spitsbergen J. M.: Pseudocapillaria tomentosa, a nematode pathogen, and associated neoplasms of zebrafish (Danio rerio) kept in research colonies. Comp. Med. 2002, 52,354-358.
  • 6. Lewis J. A., Wu C. H., Berg H., Levine J. H.: The genetics of levamisole resistance in the nematode Caenorhabditis elegans. Genetics 1980, 95, 905-928.
  • 7. Lopes W. D., dos Santos T. R., Borges Fde A., Sakamoto C. A., Soares V. E., Costa G. H., Camargo G., Pulga M. E., Bhushan C., da Costa A. J.:Anthelmintic efficacy of oral trichlorphon solution against ivermectin resistantnematode strains in cattle. Vet. Parasitol. 2009, 166, 98-102.
  • 8. Mihok T., Košuth P., Kočišova A., Pekárik L., Brtáová E., Major P.: The intestinal parasite Pseudocapillaria tomentosa (Dujardin, 1843) of the invasive fish species topmouth gudgeon, Pseudorasbora parva (Temminck and Schlegel),in Slovakia. J. Fish Dis. 2011, 34, 711-714.
  • 9. Moravec E.: Checklist of the Metazoan Parasites of Fishes of the Czech Republic and the Slovak Republik (1873-2000). Academia, Praha 2001, 168.
  • 10. Moravec E., Wolter J., Körting W.: Some nematodes and acanthocephalans from exotic ornamental freshwater fishes imported into Germany. FoliaParasitol. 1999, 46, 296-310.
  • 11. Moravec F., Ergens R., Repova R.: First record of the nematode Pseudocapillaria brevispicula (Linstow, 1873) from aquarium fishes. Folia Parasitol. 1984, 3,241-245.
  • 12. Moravec F., Gelnar M., Rehulka J.: Capillostrongyloides ancistri n. sp. (Nematoda: Capillariidae) a new pathogenic parasite of aquarium fishes inEurope. Folia Parasitol. 1987, 34, 157-161.
  • 13. Moravec F., Gut J.: Morphology of the nematode Capillaria pterophylli Heize 1933, a pathogenic parasite of some aquarium fishes. Folia Parasitol. 1982,29, 227-231.
  • 14. Moravec F., Nagasawa K., Madinabeitia I.: A new species of Capillaria (Nematoda: Capillariidae) from the intestine of the marine fish Acanthopagrusschlegelii schlegelii (Sparidae) from Japan. J. Parasitol. 2010, 96, 771-774.
  • 15. Pack M., Belak J., Boggs C., Fishman M., Driever W.: Intestinal capillariasis in zebrafish. Zebrafish Sci. Monitor 1995, 3, 1-3.
  • 16. Qian H., Robertson A. P., Powell-Coffman J. O., Martin R. J.: Levamisole resistance resolved at the single-channel level in Caenorhabditis elegans.FASEB J. 2008, 22, 3247-3254.
  • 17. Rahmati-Holasoo H., Mousavi H. A. E., Soltani M., Hosseini S. H., Ghadam M., Samani R.: Capillariosis in breeder discus (Symphysodon aeqifasciatus) inIran. J. Agri. Sci. 2010, 55, 253-259.
  • 18. Siwicki A. K., Cossarini-Dunier M., Studnicka M., Demael A.: In vivo effect of the organophosphorus insecticide trichlorphon on inmune response of carp(Cyprinus carpio). II. Effect of high doses of trichlorphon on nonspecificinmune response. Ecotox. Environ. Safety 1990, 19, 99-105.
  • 19. Taraschewski H., Renner C., Mehlhorn H.: Treatment of fish parasites. 3. Effects of levamisole-HCl, metrifonate, febendasole, mebendazole, and ivermectin on Anguillicola crassus (nematodes) pathogenic in the air bladder of eels. Parasitol. Res. 1988, 74, 281-289.
  • 20. Timi J. T., Rossin M. A., Lanfranchi A. L.: A new species of Capillaria (Nematoda: Capillariidae) parasitizing Conger orbignianus (Pisces: Congridae)from Argentina. J. Parasitol. 2006, 92, 628-630.
  • 21. Toksen E., Nemli E., Degirmenci U., Karacalar U.: The effects of azametriphos and trichlorphon on the control of Diplectanum aequans (Monogenea:Diplectanidae) infections in cultured broodstock sea bass, Dicentrarchuslabrax. Bull. Eur. Ass. Fish Pathol. 2013, 35, 144-149.
  • 22. Yamaguti S.: Nematodes of Fishes, [w:] Yamaguti S. Systema Helminthum volme III, part I. Interscience publishers, INC. New York 1961, 7-82.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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