PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2012 | 21 | 2 |

Tytuł artykułu

Occurrence, characteristics, and genetic diversity of azotobacter chroococcum in various soils of southern Poland

Autorzy

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The presence of Azotobacter sp. in soils has beneficial effects on plants, but the abundance of these bacteria is related to many factors, especially soil pH and fertility. The presented study evaluated the abundance of Azotobacter sp. in various soils of southern Poland and confirmed the relationship between soil properties and the presence of these bacteria. Diagnostic tests indicate that all isolates belonged to A. chroococcum species. The studied bacteria were present in 43% of soil samples. Restriction analysis of the bacterial Internal Transcribed Spacer region indicate that the selected isolates were identical. Two fingerprinting methods, PCR Melting Profile and Random Analysis of Polymorphic DNA, revealed high population diversity. Four (PCR MP) and five (RAPD) congruent clusters were defined. No correlation was found between the sources of strain isolation and their positions in clusters. PCR MP and RAPD techniques appeared to be useful in intraspecies differentiation of A. chroococcum.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

21

Numer

2

Opis fizyczny

p.415-424,fig.,ref.

Twórcy

autor
  • Department of Microbiology, University of Agriculture in Kraków, 24/28 Mickiewicza, 30-059 Kraków, Poland

Bibliografia

  • 1. KHOSRAVI H., SAMAR S.M., FALLAHI E., DAVOODI H., SHAHABIAN M. Inoculation of ‘Golden Delicious’ apple trees on M9 rootstock with Azotobacter improves nutrient uptake and growth indices. J. Plant Nutr. 32, 946, 2009.
  • 2. BIARI A., GHOLAMI A., RAHMANI H.A. Growth promotion and enhanced nutrient uptake of maize (Zea mays L.) by application of plant growth promoting rhizobacteria in arid region of Iran. J. Biol. Sci 8, 1015, 2008.
  • 3. BEHL R.K., NARULA N., VASUDEVA M., SATO A., SHINANO T., OSAKI M. Harnessing wheat genotype x Azotobacter strain interactions for sustainable wheat production in semi arid tropics. Tropics 15, 121, 2006.
  • 4. ORDOOKHANI K., KHAVAZI K., MOEZZI A., REJALI F. Influence of PGPR and AMF on antioxidant activity, lycopene and potassium contents in tomato. Afr. J. Agric. Res. 5, 1108, 2010.
  • 5. RODELAS B., GONZÁLEZ-LÓPEZ J., MARTÍNEZ-TOLEDO M., POZO C., SALMERON V. Influence of Rhizobium/Azotobacter and Rhizobium/Azospirillum combined inoculation on mineral composition of faba bean (Vicia faba L.). Biol. Fertil. Soils 29, 165, 1999.
  • 6. ABBASS Z., OKON Y. Plant growth promotion by Azotobacter paspali in the rizosphere. Soil Biol. Biochem. 25, 1075, 1993.
  • 7. MARTYNIUK S., MARTYNIUK M. Occurrence of Azotobacter spp. in some Polish soils. Pol. J. Environ. Stud. 12, 371, 2003.
  • 8. TCHAN Y.T., NEW P.B. Azotobacteraceae. [In:] HOLT J.G. (Ed.) Bergey’s Manual of Systematic Bacteriology Vol 1. Williams & Wilkins: Baltimore, pp. 220-229, 1989.
  • 9. MASNY A., PŁUCIENNICZAK A. Ligation mediated PCR performed at low denaturation temperatures – PCR melting profiles. Nucl. Acids Res. 31, 1, 2003.
  • 10. WILLIAMS J.G.K., KUBELIK A.R., LIVAK K.J., RAFALSKI J.A., TINGEY S.V. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl. Acids Res. 18, 6531, 1990.
  • 11. KRAWCZYK B., LEIBNER-CISZAK J., MIELECH A., NOWAK M., KUR J. PCR Melting Profile (PCR MP) – a new tool for differentiation of Candida albicans strains. BMC Infect. Dis. 9, 177, 2009.
  • 12. LARASSA J., GARCÍA-SÁNCHEZ A., AMBROSSE N., PARRA A., ALONSO J.M., REY J.M., HERMOSO-DEMENDOZA M., HERMOSO-DE-MENDOZA J. Evaluation of randomly amplified polymorphic DNA and pulsed field gel electrophoresis techniques for molecular typing of Dermatophilus congolensis. FEMS Microbiol. Lett. 240, 87, 2004.
  • 13. SMITH S., CANTET F., ANGELINI F., MARAIS A., MÉGRAUD F., BAYERDŐFFER E., MIEHLKE S. Discriminatory power of RAPD, PCR-RFLP and Southern Blot analyses of ureCD or ureA gene probes on Helicobacter pylori isolates. Z. Naturforsch. 57c, 516, 2002.
  • 14. ISO 11465. Soil quality – Determination of dry matter and water content on a mass basis – Gravimetric method. International Organization for Standardization. Geneve, Switzerland, 1999.
  • 15. SHARMA S.D., KUMAR P., RAJ H., BHARDWAJ S.K. Isolation of arbuscular mycorrhizal fungi and Azotobacter chroococcum from local litchi orchards and evaluation of their activity in the air-layers system. Sci. Hort. 123, 117, 2009.
  • 16. TROCHIMCZUK A.W., KOLARZ B.N., JERMAKOWICZ- BARTKOWIAK D. Metal ion uptake by ion + exchange-chelating resins modified with cyclohexene oxide and cyclohexene sulphide. Eur. Polym. J. 37, 559, 2001.
  • 17. OLEKSYNOWA K., TOKAJ J., JAKUBIEC J., KOMORNICKI T. Guidebook to practices with soil science and geology. part 2. Agricultural Academy in Krakow 1991 [In Polish].
  • 18. BARNES R.J.,BAXTER S.J., LARK R.M. Spatial covariation of Azotobacter abundance and soil properties: a case study using the wavelet transform. Soil Biol. Biochem. 39, 295, 2007.
  • 19. AQUILANTI L., FAVILLI F., CLEMENTI F. Comparison of different strategies for isolation and preliminary identification of Azotobacter from soil samples. Soil Biol. Biochem. 36, 1475, 2004.
  • 20. OLYUNINA L.N., MATSKOVA Y.A., GONCHAROVA T.A., GUSHINA Y.Y. Evaluation of thermal resistance of Azotobacter chroococcum 66 using atomic force microscopy. Appl. Biochem. Microbiol. 45, 38, 2009.
  • 21. ATLAS R.M., PARKS L.C. Handbook of Microbiological Media, 2nd ed., CRC Press: Boca Raton, 126, 1997.
  • 22. JENSEN M.A., WEBSTER J.A., STRAUS N. Rapid identification of bacteria on the basis of Polymerase Chain Reaction – amplified ribosomal DNA spacer polymorphisms. Appl. Environ. Microbiol. 59, 945, 1993.
  • 23. KRAWCZYK B., SAMET A., LEIBNER J., ŚLEDZIŃSKA A., KUR J. Evaluation of a PCR Melting Profile technique for bacterial strain differentiation. J. Clin. Microbiol. 44, 2327, 2006.
  • 24. FILIPEK T., KACZOR A. The dynamics of anthropogenic acidification pressure on agricultural production in Poland, [In:] KUKUŁA S. (Ed.) Protection and use of agricultural production in Poland. IUNG Puławy, Poland, pp. 35-43, 1997.
  • 25. MERCIK S. Most important soil properties and yielding on long-term static fertilising experiments in Skierniewice. Rocz. Gleboz. 44, 71, 1994.
  • 26. MYŚKOW W., WRÓBLEWSKA B., STACHYRA A., PIERZYŃSKI A. Effect of long-term organic and mineral fertilization on the biological fixation of atmospheric nitrogen and the productivity of light soils. Pam. Puł. 93, 131, 1988.
  • 27. DŐBEREINER J. Isolation and identification of aerobic nitrogen-fixing bacteria from soil and plants. in: ALEF K., NANNIPIERI P. (Eds.) Methods in Applied Soil Microbiology and Biochemistry. Academic Press: London, pp. 134-141, 1995.
  • 28. LIMMER C., DRAKE H. L. Non-symbiotic N2-fixation in acidic and pH-neutral forest soils: aerobic and anaerobic differentials. Soil Biol. Biochem. 28, 177, 1996.
  • 29. SAFARI SINEGANI A.A., SHARIFI Z. Land use effect on the occurrence and distribution of Azotobacter in Hamadan soils, Iran. Proceeding of the Fourth International Iran and Russia Conference in Agriculture and Natural Resources. Sep. 8-10, Shahrekord, Iran, pp. 614-618, 2004.
  • 30. OCAMPO J.A., BAREA J.M., MONTOYA E. Interactions between Azotobacter and “phosphobacteria” and their establishment in the rhizosphere as affected by soil fertility. Can. J. Microbiol. 21, 1160, 1975.
  • 31. VERMA V., RAJU S.C., KAPLEY A., KALIA V.C., DAGINAWALA H.F., PUROHIT H.J. Evaluation of genetic and functional diversity of Stenotrophomonas isolates from diverse effluent treatment plants. Biores. Technol. 101, 7744, 2010.
  • 32. VALDEZATE S., VINDEL A., MARTÍN-DÁVILA P., SÁNCHEZ DEL SAZ B., BAQUERO F., CANTÓN R. High genetic diversity among Stenotrophomonas maltophilia strains despite their originating at a single hospital. J. Clin. Microbiol. 42, 693, 2004.
  • 33. BHATIA R., RUPPEL S., NARULA N. NifH-based studies on azotobacterial diversity in cotton soils of India. Arch. Microbiol. 191, 807, 2009.
  • 34. BHATTACHARYA D., SARMA P.M., KRISHNAN S., MISHRA S., LAL B. Evaluation of genetic diversity among Pseudomonas citronellosis strains isolated from oily sludgecontaminated sites. Appl. Environ. Microbiol. 69, 1435, 2003.
  • 35. RENDERS N., RŐMLING U., VERBRUGH H., VAN BELKUM A. Comparative typing of Pseudomonas aeruginosa by Random Amplification of Polymorpfic DNA or Pulsed-Field Gel Electrophoresis of DNA macrorestriction fragments. J. Clin. Microbiol. 34, 3190, 1996.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-5edea927-101b-42ff-9fbd-036ca8e7af76
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.