PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2009 | 60 | 4 |
Tytuł artykułu

The effects of di-n-butyl phthalate on the germ cells of laboratory mice

Treść / Zawartość
Warianty tytułu
PL
Wpływ ftalanu di-n-butylu na komórki płciowe myszy laboratoryjnych
Języki publikacji
EN
Abstrakty
EN
Phthalate are found in the environmental samples due to their wide use in the industry as plasticizers. Di-n-butyl phthalate (DBP) is mainly used in nitrocellulose and polyvinyl acetate products as well as in personal-care products. This study was performed to investigate the influence of exposure to DBP on the quantity and quality (motility, morphology) and DNA damage (induction of micronuclei and DNA strand breaks) of male mice gametes. The estimation of DBP residues was also done. Eight weeks exposure to DBP (500 mg/kg bw and 2000 mg/kg bw) did not significantly affect testes and epididymes weights as well as sperm count. DBP clearly diminished sperm motility, enhanced frequency of abnormal sperm heads and not significantly increased DNA strand breaks in germ cells as well as frequency of micronuclei in spermatids. There were no bioacumulation of DBP in mice. Results suggest that DBP may affect the male mice germ cells.
PL
Ftalany są powszechnie wykorzystywane w przemyśle jako plastyfikatory, dlatego też często występują w próbach środowiskowych. Ftalan di-n-butylu (DBP) jest składnikiem produktów nitrocelulozowych i poliwinylowych, jak również produktów do higieny osobistej. Celem pracy było zbadanie wpływu podawania DBP na ilość i jakość gamet męskich myszy (ruchliwość, morfologia) oraz na występowanie uszkodzeń DNA (indukcja mikrojąder oraz pęknięć nici DNA). Zbadano także stężenie pozostałości DBP w gametach. Ośmiotygodniowe narażanie na DBP (500 mg/kg mc i 2000 mg/kg mc) nie powodowało istotnych zmian w ciężarze jąder i najądrzy ani w liczebności plemników. Narażenie na DBP, zwłaszcza w dawce 2000 mg/kg mc wpływało wyraźnie na zmniejszenie ruchliwości plemników i zwiększenie odsetka plemników o nieprawidłowej budowie morfologicznej. Powodowało też nieznaczne zwiększenie pęknięć nici DNA w gametach oraz zwiększenie częstości występowania mikrojąder w spermatydach. Nie wykazano bioakumulacji DBP w gonadach samców myszy. Uzyskane wyniki sugerują, że DBP może oddziaływać niekorzystnie na męskie komórki rozrodcze myszy.
Wydawca
-
Rocznik
Tom
60
Numer
4
Opis fizyczny
p.317-324,ref.
Twórcy
  • Department of Radiation Protection and Radiobiology, National Institute of Public Health - National Institute of Hygiene, 24 Chocimska str., 00-791 Warsaw, Poland
autor
  • Department of Environmental Toxicology, National Institute of Public Health - National Institute of Hygiene, Warsaw, Poland
autor
  • Department of Environmental Toxicology, National Institute of Public Health - National Institute of Hygiene, Warsaw, Poland
  • Department of Radiation Protection and Radiobiology, National Institute of Public Health - National Institute of Hygiene, 24 Chocimska str., 00-791 Warsaw, Poland
autor
  • Department of Environmental Toxicology, National Institute of Public Health - National Institute of Hygiene, Warsaw, Poland
  • Department of Environmental Toxicology, National Institute of Public Health - National Institute of Hygiene, Warsaw, Poland
Bibliografia
  • 1. Albro P.W., Hass J. R., Peck C.C, Jordan S.T., Schroeder J.: Applications of isotope differentiation for metabolic studies with di-(2-ethylhexyl) phthalate. J. Environ. Sci. Health B 1982, 17, 701-14.
  • 2. Anderson D., Yu T.W., Phillips B.J., Schmezer P.: 1994. The effects of various antioxidants and other modfying agents on oxygen-radical-generated damage in human lymphocytes in Comet assay. Mutat. Res. 1994, 307, 261-71
  • 3. ATSDR. Toxicological profile for Di-n-butyl phthalate. Agency for Toxic Substances and Disease Registry, Atlanta GA USA, 2001 Available at: http:/www.atsdr.cdc.gov/toxprofiles/tp135.html.
  • 4. ATSDR. Toxicological profile for di-(2-ethylhexyl) phthalate (DEHP). Agency for Toxic Substances and Disease Registry, Atlanta GA USA, 2002 Available at: http:/www.atsdr.cdc.gov/toxprofiles/tp9.html.
  • 5. Boekelheide K., Johnson K.J., Richburg J.H.: Sertoli cell toxicants, In: Skinner M.K., Griswold M.D. (Eds.), Sertolli cell biology, Elsevier Academic Press, San Diego, 2004.
  • 6. CIRC (Cosmetic Ingradient Review Committee). Final report on the safety assessment of dibutyl phthalate, dimethyl phthalate and diethyl phthalate. J. Am. Coll. Toxicol. 1985, 4, 267-303.
  • 7. Cliet I., Melcion C., Cordier A.: Lack of predictivity of bone marrow micronucleus test versus testis micronucleus test: comparison with four carcinogens. Mutat. Res. 1993, 292, 105-111.
  • 8. Colborn T., Clement C.: Advances in modern environmental toxicology. Vol XXI Chemically-induced alterations in sexual and functional development: the wildlife/human connection. Princeton Scientific Publishing Co Inc Princeton, NJ, 1992.
  • 9. Dalgaard M., Nellemann C., Lam H.R., Sorensen J.K., Lodefoped O.: The acute effects of mono(2-ethylhexyl)phthalate (MEHP) on testes of pubertal Wistar rats. Toxicol. Lett. 2001, 122, 69-79.
  • 10. Duty S.M., Silva M.J., Barr D.B., Brock J., Ryan L., Chen Z., Herrick R.F., Christiani D.C., Hauser R.: Phthalate exposure and human semen parameters. Epidemiology 2003a, 14, 269-77.
  • 11. Duty S.M., Singh N.P., Silva M.J., Barr D.B., Brock J.W., Ryan L., Herrick R.F., Christiani D.C., Hauser R.: The relationship between environmental exposure to phthalates and DNA damage in human sperm using the neutral comet assay, Environ. Health Perspect. 2003b, 1119, 1164-69.
  • 12. Ema M., Amano H., Ogawa Y.: Characterization of the developmental toxicity of di-n-butyl phthalate in rats. Toxicology 1994, 86, 163-174.
  • 13. Ema M., Amano H., Itami T., Kawasaki H.: Teratogenic evaluation of di-n-butyl phthalate in rats. Toxicol. Lett. 1993, 69, 197-203.
  • 14. Ema M., Kurosaka R., Amano H., Ogawa Y.: Comparative developmental toxicity of n-butylbenzyl phthalate and di-n-butyl phthalate in rats. Arch. Environ. Contam. Toxicol. 1995, 28, 223-28.
  • 15. European Commission (2005). Press Release 5th July 2005. Available at: http://www.europa.eu.int/ rapid/pressReleasesAction.do?reference=IP?05/ 838&format=HTML&taged=1&language=EN &guilLanguage=en.
  • 16. Fukuoka M., Tanimoto T., Zhou Y., Kawasaki N., Tanaka A., Ikemoto I., Machida T.: Mechanism of testicular atrophy induced by di-n-butyl phthalate in rats. Part 4. Changes in the activity of succinate dehydrogenase and the levels of transferin and ferritin in the Sertoli and germ cells. J. Appl. Toxicol. 1989, 13, 241-46.
  • 17. Gangolli S.D.: Testicular effects of phthalate esters. Environ. Health Perspect. 45, 77-84.
  • 18. Gray L.E. Jr., Satby J., Furr J., Price M., Veeramachaneni D.N., Parks L.: Perinatal exposure to the phthalates DEHP, BBP, and DOTP, alterssexual differentiation of the male rat. Toxicol. Sci. 2000, 58, 350-365.
  • 19. Hardell L., Olson C.G., Fredricson M.: Occupational exposure to polyvinyl chloride as a risk factor for testicular cancer evaluated in a case-counted study. Int. J. Cancer 1997, 73, 828-30.
  • 20. Harrison A., and Moore P.C.: Reduction in sperm count and increase in abnormal sperm in the mouse following X-radiation or injection of 22Na, Health Physics 1980, 39, 219-224.
  • 21. Hauser R. and Calafat A.M.: Phthalates and human health. Occupat. Environ. Med. 2005, 62, 806-818.
  • 22. Hauser R., Meeker J.D., Singh N.P., Silva M.J., Ryan L., Duty S., Calafat A.M.: 2007. DNA damage in human sperm is related to urinary levels of phthalate monoester and oxidative metabolites. Hum. Reprod. 2007, 22, 88-95.
  • 23. Kastenbaum M.A. and Bowman K.O.: Tables for determining the statistical significance of mutation frequencies. Mutat. Res. 1970, 9, 527-49.
  • 24. Kavlock R., Boekelheide K., Chapin R., Cunningham M., Faustman E., Foster P., Golub M., Henderson R., Hinberg I., Little R., Seed J., Shea K., Tabacova S., Tyl R., Wiliams P., Zacharewski T.: NTP Center for Evaluation of Risks to Human Reproduction: phthaltes expert panel report on the reproductive and developmental toxicity of di-n-butyl phthalate. Reprod. Toxicol. 2002, 16, 453-87.
  • 25. Kim H.S., Kim T.S., Shin J.H., Moon H.J., Kang I.H., Kim I.Y., Oh J.Y., Han S.Y.: Neonatal exposure to di-(nbutyl) phthalate (DBP) alters male reproductive-tract development. J. Toxicol. Environ. Health A 2004, 67, 2045-60.
  • 26. Kleymenova E., Swanson C., Boekelheide K., Gaido K.W.: Exposure in utero to di(n-butyl) phthalate alters the vimentin cytoskeleton of fetal rat Sertoli cells and disruptors Sertoli cell-gonocyte contact. Biol. Reprod. 2005, 73, 482-90.
  • 27. Końca K., Lankoff A., Banasik A., Lisowska H., Kuszewski T., Góźdź S., Koza Z., Wójcik A.: A cross-platform public domain PC image-analysis program for comet assay. Mutat. Res. 2003, 534, 15-20.
  • 28. Koo H.J., Lee B.M.: Estimated exposure to phthalates in cosmetics and risk assessment. J. Toxicol. Environ. Health Part A 2004, 67, 1901-1914.
  • 29. Ljungvall K., Tienpont B., David F., Magnusson U., Törneke K.: Kinetics of orally administered di(2-ethylhexyl) phthalate and its metabolite, mono (2-ethylhexyl) phthalate, in male pigs. Arch. Toxicol. 2004, 78, 384-89.
  • 30. Marsman D.: 1995. NTP technical report on the toxicity studies of dibutyl phthalate (CAS No.84-74-2) administered in feed to F344/N rats and B6C3F1 mice. Toxicity Report Series 1995, 30, 1-G5.
  • 31. Meistrich M.L., Bruce W.R., Clermont Y.: Cellular composition of fractions of mouse testis following velocity sedimentation separation. Exp. Cell Res. 1973, 79, 213-27.
  • 32. Mendes A.J.J.: The endocrine disrupters: a major medical challenge. Food Chem. Toxicol. 2002, 40, 781-88.
  • 33. Mes J., Coffin D.E., Campbel D.S.: Di-n-buthyl and di- 2-ethyl-hexyl phthalate in human adipose tissue. Bull Environ. Contam. Toxicol. 1974, 12, 721-25.
  • 34. Moore N.P.: The oestrogenic potential of the phthalate esters. Reprod. Toxicol. 2000, 14, 183-192.
  • 35. Mylchreest E., Cattley R.C., Foster P.M.: Male reproductive tract malformations in rat following gestational and lactational exposure to di(n-butyl) phthalate; an antiandrogenic mechanism? Toxicol. Sci. 1998, 43, 47-60.
  • 36. NTP. NTP-CERHR Expert Panel Report on Butyl Benzyl Phthalate. Research Triangle Park, NC-National Toxicology Program, Center for the Evaluation of Risks to Human Reproduction. 2000, 1-37.
  • 37. Rawland I.R.: Metabolism of di-(2-ethylhexyl)phthalate by the contents of the alimentary tract of the rat. Food Cosmet. Toxicol. 1974, 12, 293-302.
  • 38. Richburg J.H. and Boekelheide K.: Mono-(2- ethylhexyl)phthalate rapidly alters both Sertoli cell vimentin filaments and germ cell apoptosis in young rat testes. Toxicol. Appl. Pharmacol. 1996, 137, 42-50.
  • 39. Schantz S.L., Wildholm J.J.: Congenitive effects of endocrine- disrupting chemicals in animals. Environ. Health Perspect. 2001, 109: 1197-1206.
  • 40. Schmid P. and Schlatter C.: Excretion and metabolism of di-(2- ethylhexyl)-phthalate in man. Xenobiotica 1985, 15, 251-56.
  • 41. Searle A.G. and Beechey C.V.: Sperm count, egg-fertilization and dominant lethality after X-irradiation of mice. Mutat. Res. 1974, 22, 69-74.
  • 42. Sharpe R.M., Fisher J.S., Millar M.M., Jobling S., Sumpter J.P.: Gestational and lactational exposure of rats to xenoestrogens results in reduced testicular size and sperm production. Environ. Health Perspect. 1995, 103, 1136-43.
  • 43. Shiota K., Nishimura H.: Teratogenicity of di(2- ethylhexyl)phthalate and di-n-butyl phthalate (DBP) in mice. Environ. Health Perspect. 1982, 45, 65-70.
  • 44. Singh N.P., Mc Coy M., Tice R.R., Schneider E.L.: A simple technique for quantitation of low level of DNA damage in individual cells. Exp. Cell Res. 1988, 175, 184-191.
  • 45. Tates A.D., Dietrich A.J.J., de Vogel N., Neuteboom I., Bos A.: A micronucleus method for detection of meiotic micronuclei in male germ cells of mammals. Mutat. Res. 1983, 121, 131-138.
  • 46. U.S. EPA (United States Environmental Protection Agency). Office of Ground Water and Drinking Water. Drinking Water and Health, 1999, (http://www.epa.gov/ogwdw000/dwh/c-soc/Phthalat.html)
  • 47. Wezel A.P. van, Vlaardingen P. van, Posthumus R., Crommentuijn G.H., Sijm D.T.H.M.: Environmental risk limits for two phthalates with special emphasis on endocrine disruptive properties. Ecotoxicol. Environ. Safety 2000, 46, 305-321.
  • 48. White R.D., Carter D.E., Earnest D., Mueller J.: Absorption and metabolism of three phthalate diesters by the rat small intestine. Food Toxicol. 1980, 18, 383-86.
  • 49. Williams D.T.: Dibuthyl and di-(2-ethylhesyl) phthalate in fish. J. Agric. Food Chem. 1973, 21, 1128-34.
  • 50. Williams D.T., Blanchfield B.: The retention, distribution, excretion, and metabolism of dibutyl phthalate -14C in the rat. J. Agric. Food Chem. 1975, 23, 854-58.
  • 51. Williams J., and Foster P.M.D.: The production of lactation and pyruvate sensitive indices of altered rat Sertoli cells function in vitro following the addition of various testicular toxicants. Toxicol. Appl. Pharmacol. 1988, 94, 160-70.
  • 52. Wine R.N., Li L.H., Barners L.H., Gulati, D.K., Chapin, R.E.: Reproductive toxicity of di-n-butylphthalate in a continuous breeding protocol in Sprague-Dawley rats. Environ. Health Perspect. 1997, 105, 102-107.
  • 53. Working P.K., Bus J.S., Hamm T.E. Jr.: Reproductive effects of inhaled methyl chloride in the male Fisher 344 rat. II. Spermatogonial toxicity and sperm quality. Toxicol. Appl. Pharm. 1985, 77, 144-57.
  • 54. Wyrobek A.J., and Bruce W.R.: Chemical induction of sperm abnormalities in mice. Proceed. Nat. Acad. Sci. USA 1975, 72, 4425-4429.
  • 55. Zhang Y., Jiang X. Chen B.: Reproductive and developmental toxicity in F1 Sprague-Dawley male rats exposed to di-n-butyl phthalate in utero and during lactation and determination of its NOAEL. Reprod. Toxicol. 2004, 18, 669-76.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-5ebcd7f5-fa84-4650-b46c-76275587656a
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.