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2010 | 32 | 1 |
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Effect of heavy metals on root growth and peroxidase activity in barley root tip

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In the present work, we investigated the alteration of oxidative and peroxidative activities of peroxidases (PODs) along the longitudinal root axis of barley seedlings during heavy metal (HM; e.g., Cd, Cu, Hg, Ni, Pb) treatment. Analysis of the individual root segments revealed that all of the analyzed HMs caused an increase of guaiacol-POD activity, however to a different extent and spatial distribution. Cd-induced ferulic acid POD activity was observed along the whole root tip (RT), while Cu and Hg caused its increase in the meristematic zone and Ni mainly at the end of the differentiation zone of RT. The activation of coniferyl alcohol POD by HMs was detected along the whole RT. HM-induced hydrogen peroxide-generating POD activity was localized mainly to the elongation zone of RT. Elevated chlorogenic acid POD activity was observed in the meristematic zone and at the end of the differentiation zone of RTs. The activation of several PODs is probably associated with enhanced H₂O₂ production and lignification as a defense response of roots to several HM, to prevent their uncontrolled flux. On the other hand, this defense response is accompanied by root growth inhibition, due to the enhanced rigidification of cell wall and accelerated differentiation of RTs.
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  • Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences
  • Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences
  • Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences
  • Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences
  • Baker CJ, Deahl K, Domek J, Orlandi EW (2000) Scavenging of H₂O₂ and production of oxygen by horseradish peroxidase. Arch Biochem Biophys 382:232–237
  • Boudet AM, Lapierre C, Grima-Pettenati J (1995) Biochemistry and molecular biology of lignification. New Phytol 129:203–236
  • Chance B, Maehly AC (1955) Assay of catalase and peroxidases. In: Colowick SD, Kaplan NO (eds) Methods in enzymology, vol 2. Academic Press, New York, pp 764–775
  • Chaoui A, Jarrar B, El Ferjani E (2004) Effects of cadmium and copper on peroxidase, NADH oxidase and IAA oxidase activities in cell wall, soluble and microsomal membrane fractions of pea roots. J Plant Physiol 161:1225–1234
  • Chen EL, Chen YA, Chen LM, Liu ZH (2002) Effect of copper on peroxidase activity and lignin content in Raphanus sativus. Plant Physiol Biochem 40:439–444
  • De Marco A, Roubelakis-Angelakis KA (1996) The complexity of enzymic control of hydrogen peroxide concentration may affect the regeneration potential of plant protoplasts. Plant Physiol 110:137–145
  • Dunand C, Crevecoeur M, Penel C (2007) Distribution of superoxide and hydrogen peroxide in Arabidopsis root and their influence on root development: possible interaction with peroxidases. New Phytol 174:332–341
  • Ďurčeková K, Huttová J, Mistrík I, Ollé M, Tamás L (2007) Cadmium induces premature xylogenesis in barley roots. Plant Soil 290:61–68
  • Ederli L, Reale L, Ferranti F, Pasqualini S (2004) Responses induced by high concentration of cadmium in Phragmites australis roots. Physiol Plant 121:66–74
  • Fry SC (1986) Cross-linking of matrix polymers in the growing cell walls of angiosperms. Annu Rev Plant Physiol 37:165–186
  • Gajewska E, Slaba M, Andrzejewska R, Sklodowska M (2006) Nickel-induced inhibition of wheat root growth is related to H₂O₂ production, but not to lipid peroxidation. Plant Growth Regul 49:95–103
  • Gapper C, Dolan L (2006) Control of plant development by reactive oxygen species. Plant Physiol 141:341–345
  • Goldberg R, Liberman M, Mathieu C, Pierron M, Catesson AM (1987) Development of epidermal cell wall peroxidases along the mung bean hypocotyl: possible involvement in the cell wall stiffening process. J Exp Bot 38:1378–1390
  • González LF, Rojas MC (1999) Role of wall peroxidases in oat growth inhibition by DIMBOA. Phytochemistry 50:931–937
  • Grabber JH, Hatfield RD, Ralph J, Zon J, Amrhein N (1995) Ferulate cross-linking in cell walls isolated from maize cell suspensions. Phytochemistry 40:1077–1082
  • Grace SC, Salgo MG, Pryor WA (1998) Scavenging of peroxynitrite by a phenolic/peroxidase system prevents oxidative damage to DNA. FEBS Lett 426:24–28
  • Hegedüs A, Erdei S, Horvath G (2001) Comparative studies of H₂O₂ detoxifying enzymes in green and greening barley seedlings under cadmium stress. Plant Sci 160:1085–1093
  • Hiraga S, Yamamoto K, Ito H, Sasaki K, Matsui H, Honma M, Nagamura Y, Sasaki T, Ohashi Y (2000) Diverse expression profiles of 21 rice peroxidase genes. FEBS Lett 471:245–250
  • Hiraga S, Sasaki K, Ito H, Ohashi Y, Matsui H (2001) A large family of class III plant peroxidases. Plant Cell Physiol 42:462–468
  • Hsu YT, Kao CH (2003) Role of abscisic acid in cadmium tolerance of rice (Oryza sativa L.) seedlings. Plant Cell Environ 26:867–874
  • Ishii T (1997) Structure and functions of feruloylated polysaccharides. Plant Sci 127:111–127
  • Kamisaka S, Takeda S, Takahashi K, Shibata K (1990) Diferulic and ferulic acid in the cell wall of Avena coleoptiles: their relationships to mechanical properties of the cell wall. Physiol Plant 78:1–7
  • Kennedy CD, Gonsalves FAN (1989) The action of divalent Zn, Cd, Hg, Cu and Pb ions on the ATPase activity of a plasma membrane fraction isolated from roots of Zea mays. Plant Soil 117:167–175
  • Lin CC, Kao CH (2001a) Cell wall peroxidase against ferulic acid, lignin, and NaCl-reduced root growth of rice seedlings. J Plant Physiol 158:667–671
  • Lin CC, Kao CH (2001b) Abscisic acid-induced changes in cell wall peroxidase activity and hydrogen peroxide level in roots of rice seedlings. Plant Sci 160:323–329
  • Lin CC, Chen LM, Liu ZH (2005) Rapid effect of copper on lignin biosynthesis in soybean roots. Plant Sci 168:855–861
  • Lindsay SE, Fry SC (2008) Control of diferulate formation in dicotyledonous and gramineous cell suspension cultures. Planta 227:439–452
  • Liszkay A, Kenk B, Schopfer P (2003) Evidence for the involvement of cell wall peroxidase in the generation of hydroxyl radicals mediating extension growth. Planta 217:658–667
  • Lucena MA, Romero-Aranda R, Mercado JA, Cuartero J, Valpuesta V, Quesada MA (2003) Structural and physiological changes in the roots of tomato plants over-expressing a basic peroxidase. Physiol Plant 118:422–429
  • Mäder M, Ungemach J, Schlob P (1980) The role of peroxidase isoenzyme groups of Nicotiana tabacum in hydrogen peroxide formation. Planta 147:467–470
  • Mika A, Minibayeva F, Beckett R, Lüthje S (2004) Possible functions of extracellular peroxidases in stress-induced generation and detoxification of active oxygen species. Phytochem Rev 3:173–193
  • Parra-Lobato MC, Alvarez-Tinaut MC, Gomez-Jimenez MC (2007) Cloning and characterization of a root sunflower peroxidase gene putatively involved in cell elongation. J Plant Physiol 164:1688–1692
  • Passardi F, Penel C, Dunand C (2004) Performing the paradoxical: how plant peroxidases modify the cell wall. Trends Plant Sci 9:534–540
  • Passardi F, Tognolli M, De Meyer M, Penel C, Dunand C (2006) Two cell wall-associated peroxidases from Arabidopsis influence root elongation. Planta 223:965–974
  • Patykowski J, Urbanek H (2003) Activity of enzymes related to H₂O₂ generation and metabolism in leaf apoplastic fraction of tomato leaves infected with Botrytis cinerea. J Phytopathol 151:153–161
  • Pritchard J (1994) The control of cell expansion in roots. New Phytol 127:3–26
  • Ranieri A, Castagna A, Scebba F, Careri M, Zagnoni I, Predieri G, Pagliari M, Sanita di Toppi L (2005) Oxidative stress and phytochelatin characterisation in bread wheat exposed to cadmium excess. Plant Physiol Biochem 43:45–54
  • Schopfer P, Plachy C, Frahry G (2001) Release of reactive oxygen intermediates (superoxide radicals, hydrogen peroxide, and hydroxyl radicals) and peroxidase in germinating radish seeds controlled by light, gibberellin, and abscisic acid. Plant Physiol 125:1591–1602
  • Sharma SS, Dietz K-J (2008) The relationship between metal toxicity and cellular redox imbalance. Trends Plant Sci 14:43–50
  • Siegel BZ (1993) Plant peroxidases: an organismic perspective. Plant Growth Regul 12:303–312
  • Tabuchi A, Matsumoto H (2001) Changes in cell wall properties of wheat (Triticum aestivum) roots during aluminum-induced growth inhibition. Physiol Plant 112:353–358
  • Takahama U, Oniki T (1997) A peroxidase/phenolics/ascorbate system can scavenge hydrogen peroxide in plant cells. Physiol Plant 101:845–852
  • Welinder KG, Justesen AF, Kjærsgård IVH, Jensen RB, Rasmussen SK, Jespersen HM, Duroux L (2002) Structural diversity and transcription of class III peroxidases from Arabidopsis thaliana. Eur J Biochem 269:6063–6081
  • Yamasaki H, Grace SC (1998) EPR detection of phytophenoxyl radicals stabilized by zinc ions: evidence for the redox coupling of plant phenolics with ascorbate in the H₂O₂–peroxidase system. FEBS Lett 422:377–380
  • Zancani M, Nagy G (2000) Phenol-dependent H₂O₂ breakdown by soybean root plasma membrane-bound peroxidase is regulated by ascorbate and thiols. J Plant Physiol 156:295–299
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