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2013 | 22 | 2 |

Tytuł artykułu

Resveratrol affects the lipid profile but not antioxidant enzymes gene expression in rats fed hypercholesterolaemic diet

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The objective of this study was to evaluate the effect of adding resveratrol (RSV) to a hypercholesterolaemic diet on the lipid profile, activity of antioxidant enzymes and their mRNA gene expression, as well as lipid concentration in selected organs of Wistar rats. Animals were divided into four groups and fed experimental diets for 8 weeks. The negative control group (NC) was fed AIN-93G diet. The positive control group (PC) received a hypercholesterolaemic diet (AIN-93G + 0.1% cholesterol, 7% butter). The other groups were fed PC diets supplemented with 0.05% and 0.1% RSV, respectively. An addition to the PC diet RSV (0.05 and 0.1%, respectively) significantly decreased concentration of total cholesterol (TC) and LDL-cholesterol in serum of rats compared to the PC group. The triacylglycerols (TAG) level was significantly lower in serum of rats fed with 0.05% of RSV in comparison to other experimental groups. Activities of antioxidant enzymes and their mRNA gene expression were not affected by RSV. In conclusion, relatively high doses of RSV were sufficient in lowering TC and LDL-cholesterol, and TAG and oxidative stress without involving naturally occurring mechanisms in rats.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

22

Numer

2

Opis fizyczny

p.137-143,ref.

Twórcy

autor
  • Department of Human Nutrition, Agricultural University of Krakow, Balicka 122, 30-149 Krakow, Poland
  • Department of Human Nutrition, Agricultural University of Krakow, Balicka 122, 30-149 Krakow, Poland
  • Department of Human Nutrition, Agricultural University of Krakow, Balicka 122, 30-149 Krakow, Poland

Bibliografia

  • Ahn J., Cho I., Kim S., Kwon D., Ha T., 2008. Dietary resveratrol alters lipid metabolism-related gene expression of mice on an atherogenic diet. J. Hepatol. 49, 1019-1028
  • Aribal P., Kavas G.O., Coban U., Elhan A.H., 2009. How does resveratrol change some metabolic and circulatory parameters? A preliminary study. Adv. Clin. Exp. Med. 18, 323–328
  • Artaud-Wild S.M. Connor S.L., Sexton G., Connor W.E., 1993. Differences in coronary mortality can be explained by differences in cholesterol and saturated fat intakes in 40 countries but not in France and Finland. A paradox. Circulation 88, 2771–2779
  • Aubin M.C., Lajoie C., Clément R., Gosselin H., Calderone A., Perrault L.P., 2008. Female rats fed a high-fat diet were associated with vascular dysfunction and cardiac fibrosis in the absence of overt obesity and hyperlipidemia: therapeutic potential of resveratrol. J. Pharmacol. Exp. Ther. 325, 961–968
  • Azorín-Ortuño M., Yáñez-Gascón M.J., Gonzáles-Sarrías Larrosa M., Vallejo F., Pallarés F.J., Lucas R., Morales J.C., Tomás-Barberáan F.A., García-Conesa M.-T., Espín J.C., 2012. Effect of long-term consumption of low doses of resveratrol on diet-induced mild hypercholesterolemic in pigs: a transcriptomic approach to disease prevention. J. Nutr. Biochem. 23, 829–837
  • Cho S.J., Jung U.J., Choi M.S., 2012. Differential effects of low-dose resveratrol on adiposity and hepatic steatosis in diet-induced obese mice. Brit. J. Nutr. 14, 1–10
  • Coventry B.J., Ashdown M.L., Quinn M.A., Markovic S.N., YatomiClarke S.L., Robinson A.P., 2009. CRP identifies homeostatic immune oscillations in cancer patients: a potential treatment targeting tool? Journal Translational Medicine 30, 102 doi:10.1186/1479-5876-7-102
  • de Man J.M., 1964. Determination of fatty acid composition of milk fat by dual column temperature programmed gas liquid chromatography. J. Dairy Sci. 47, 546–547
  • Domagała J., Sady M., Grega T., Pustkowia H., Forkiewicz A., 2010. The influence of cheese type and fat extraction method on the content of conjugated linoleic acid. J.Food Compos. Anal. 23, 238–243
  • Folch J., Lees M., Sloane Stanley G.H., 1957. A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem. 226, 497–509
  • Fortuna T., Juszczak L., Sobolewska-Zielińska J., 2003. Basic of food analysis. Agric. Univ. of Krakow Press (in Polish)
  • Friedewald W.T., Fredrick D.S., Levy R.I., 1972. Estimation of concentration of low-density lipoprotein cholesterol in plasma, without use of preparative ultracentrifuge. Clin. Chem. 18, 499–502
  • Juan M.E., Gonzalez-Pons E., Munuera T., Ballester J., Rodrıguez-Gil J.E., Planas J.M., 2005. Trans-resveratrol, a natural antioxidant from grapes, increases sperm output in healthy rats. J. Nutr. 135, 757–760
  • Kaput J.K., 2004. Diet-disease gene interaction. Nutrition 20, 26–31
  • Kaur G., Rao L.V.M., Agraval A., Pendurthi R., 2007. Effect of wine phenolics on cytokine-induced C-reactive protein expression. J. Thromb. Haemost. 5, 1309–1317
  • Kim S., Jin Y., Choi Y., Park T., 2011. Resveratrol exerts anti-obesity effect via mechanism involving down-regulation of adipogenic and inflammation processes in mice. Biochem. Pharmacol. 81,1343–1351
  • Kitada M., Kume S., Imaizumi N., Koya D., 2011. Resveratrol improves oxidative stress and protects against diabetic nephropathy through normalization of Mn-SOD dysfunction in AMPK/SIRT1-independent pathway. Diabetes 60, 634–643
  • Kopeć A., Piątkowska E., Leszczyńska T., Koronowicz A., 2013. Effect of long term administration of resveratrol on lipid concentration in selected organs and liver’s histology in rats fed high fructose diet. J. Funct. Foods 5, 299–305
  • Lagouge M., Argmann C., Gerhart-Hines Z., et al., 2006. Resveratrol improves mitochondrial function and protects against metabolic disease by activating SIRT1 and PGC-1 alpha. Cell 127, 1109–1122
  • Leiro J.,Varela M., Piazzon M.C., Arranz J.A., Noya M., Lamas J., 2010. The anti-inflammatory activity of the polyphenol resveratrol may be partially related to inhibition of tumor necrosis factor-a (TNF-a) pre-mRNA splicing. Mol. Immunol. 47, 1114–1120
  • Louis X.L., Thandapilly S.J., Mohankumar S.K., Yu L., Taylor C.G., Zahradka P., Netticadan T., 2011. Treatment with low-dose resveratrol reverses cardiac impairment in obese prone but not in obese resistant rats. J. Nutr. Biochem. 11, 1–7
  • Macarulla M.T., Alberdi G., Gómez S., Tueros I., Bald C., Rodríguez V.M., Martínez J.A., Portillo M. P., 2009. Effects of different doses of resveratrol on body fat and serum parameters in rats fed a hypercaloric diet. J. Physiol. Biochem. 65, 369–376
  • Penumathsa S.V., Koneru S., Samuel S.M., Maulik G., Bagchi D., Yet S.F., Menono V.P., Maulik N., 2008. Strategic targets to induce neovascularization by resveratrol in hypercholesterolemic rat myocardium: role of caveolin-1, eNOS, HO-1 and VEGF. Free Radical. Biol. Med. 45, 1027–1034
  • Pisulewski P.M., Kopeć A., Cieślik E., 2002. A note on the development of rat model for cholesterol and lipoprotein metabolism a short report. Pol. J. Food Nutr. Sci. 4, 63–66
  • Reeves P.G., 1997. Components of the AIN-93 diets as improvements in the AIN-76A diet. J. Nutr. 127, 838–841
  • Rocha K.K.R., Souza G.A., Ebaid G.X., Seiva F.R.F., Cataneo A.C., Novelii E.L.B., 2009. Resveratrol toxicity: effect on risk factors for atherosclerosis and hepatic oxidative stress in standard and high-fat diet. Food Chem. Toxicol. 47, 1362–1367
  • Schmatz R., Perreira L.B., Stefanello N. et al., 2012. Effect of resveratrol on biomarkers of oxidative stress and on the activity of delta aminolevulinic acid dehydrates in liver and kidney of streptozotocin-induced diabetes rats. Biochimie 94, 374–383
  • Tauriainen E., Luostarinen M., Martonen E., Finckenberg P., Kovalainen M., Huotari A., Herzig K.H., Lecklin A., Mervaala E., 2011. Distinct effects of calorie restriction and resveratrol on dietinduced obesity and fatty liver formation. J. Nutr. Metab. doi:10.1155/2011/525094
  • Tecator Foss Application note no. ASN 3131
  • Wagner A.E., Boesch-Saadatmani Ch., Breckwold D., Schrander Ch., Schmelzer C., Döring F., Hashida K., Hori S., Rimbach G., 2011. Ascorbic acid partly antagonized resveratrol mediated heme oxygenase-1 but not paraoxogenase-1 induction in cultured hepatocytes-role of the redox-regulated transcription factor Nrf2. BMC Complement. Altern. Med. 11,1 doi:10.1186/1472-6882-11-1
  • Yamagishi K., Iso H., Kokubo Y., Saito I., Yatsuya H., Ishihara J., Inoue M., Tsugane S., 2013. Dietary intake of saturated fatty acids and incident stroke and coronary heart disease in Japanese communities: the JPHC study. Eur. Heart J. doi: 10.1093/eurheartj/eht043.
  • Yuan Y.V., Kitts D.D., 2003. Dietary (n-3) fat and cholesterol alter tissue antioxidant enzymes and susceptibility to oxidation in SHR and WKY rats. J. Nutr. 133, 679–688
  • Yuan Y.V., Kitts D.D., Godin D.V., 1998. Variations in dietary fat and cholesterol intakes modify antioxidant status of SHR and WKY rats. J. Nutr. 128, 1620–1630
  • Zawistowski J., Kopeć A., Kitts D.D., 2009. Effect of a black rice extract (Oryza sativa L. indica) on cholesterol levels and plasma lipid parameters in Wistar Kyoto rats. J. Funct. Foods 1, 50–56

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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