Protocol for acclimatization of in vitro cultured Potamogeton praelongus - aspect of plantlet size and type of substrate

• Autorzy: Prausova R. , Kozelkova Z. , Safarova L.
• Języki publikacji: EN

Abstrakty

EN

The aim of the experiment was to determine suitable substrate type and optimal plant size for transfer of plantlets from in vitro to ex vitro under experimental outdoor conditions. Tests focused on the effect of substrate type (muddy and sandy) and starting size of plantlets gained through in vitro seed germination (0-3, 3.1-5,5.1-6, 6.1-10 cm) on plant growth. Three parameters (fresh weight, length, and the number of leaves) were compared to evaluate growth. Basic water parameters in experimental water tanks were regularly measured (pH, temperature, electrical conductivity, shadow intensity) and controlled to reach similar conditions to those in the natural habitat of this species. Overwintering was studied in a cellar with newly defined size categories (<6, 6.1-8, 8.1-10, 10.1-12, 12.1-15 cm). Both substrate type and starting size of plantlets significantly impacted growth. Plantlets grew better in the muddy substrate while a 100% success rate of rooting was gained with a starting size of 6.1-10 cm in both substrates. The biggest increase in fresh weight was observed with a starting size of 3.1-5 cm and 5.1-6 cm in both substrates. The greatest increase in fresh weight was observed in plants with a starting size of 3.1-5 cm in the muddy substrate (more than 95% increase). The best overwintering results were gained in the 6.1-8 cm size category.

Słowa kluczowe

EN

acclimatization; in vitro culture; Potamogeton praelongus; plant tissue culture; growth experiment; plantlet size; substrate type

• Wydawca: -
• Czasopismo: Acta Societatis Botanicorum Poloniae
• Rocznik: 2015
• Tom: 84
• Numer: 1
• Opis fizyczny: p.35-41,fig.,ref.

Twórcy

autor

Prausova R.

• Department of Biology, Faculty of Science, University of Hradec Kralove, 500 02 Hradec Kralove, Czech Republic

autor

Kozelkova Z.

• Department of Biology, Faculty of Science, University of Hradec Kralove, 500 02 Hradec Kralove, Czech Republic

autor

Safarova L.

• East Bohemian Museum in Pardubice, Zamek 2, 530 02 Pardubice, Czech Republic

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Identyfikatory

DOI

10.5586/asbp.2015.003