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2015 | 84 | 1 |
Tytuł artykułu

Protocol for acclimatization of in vitro cultured Potamogeton praelongus - aspect of plantlet size and type of substrate

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The aim of the experiment was to determine suitable substrate type and optimal plant size for transfer of plantlets from in vitro to ex vitro under experimental outdoor conditions. Tests focused on the effect of substrate type (muddy and sandy) and starting size of plantlets gained through in vitro seed germination (0-3, 3.1-5,5.1-6, 6.1-10 cm) on plant growth. Three parameters (fresh weight, length, and the number of leaves) were compared to evaluate growth. Basic water parameters in experimental water tanks were regularly measured (pH, temperature, electrical conductivity, shadow intensity) and controlled to reach similar conditions to those in the natural habitat of this species. Overwintering was studied in a cellar with newly defined size categories (<6, 6.1-8, 8.1-10, 10.1-12, 12.1-15 cm). Both substrate type and starting size of plantlets significantly impacted growth. Plantlets grew better in the muddy substrate while a 100% success rate of rooting was gained with a starting size of 6.1-10 cm in both substrates. The biggest increase in fresh weight was observed with a starting size of 3.1-5 cm and 5.1-6 cm in both substrates. The greatest increase in fresh weight was observed in plants with a starting size of 3.1-5 cm in the muddy substrate (more than 95% increase). The best overwintering results were gained in the 6.1-8 cm size category.
Wydawca
-
Rocznik
Tom
84
Numer
1
Opis fizyczny
p.35-41,fig.,ref.
Twórcy
autor
  • Department of Biology, Faculty of Science, University of Hradec Kralove, 500 02 Hradec Kralove, Czech Republic
autor
  • Department of Biology, Faculty of Science, University of Hradec Kralove, 500 02 Hradec Kralove, Czech Republic
autor
  • East Bohemian Museum in Pardubice, Zamek 2, 530 02 Pardubice, Czech Republic
Bibliografia
  • 1.Vöge M. Die Entwicklung von Potamogeton praelongus im Großensee bei Hamburg. Tuexenia. 1992;12:275-284.
  • 2.Kaplan Z. Potamogeton L. - rdest. In Chrtek J (jun.), Kaplan Z, Štěpánková J, editors. Květena České republiky. Prague: Academia; 2010. p. 330-378. (vol 8).
  • 3.Šumberová K. Vegetace vodních rostlin zakořeněných ve dně (Pota-metea). In: Chytrý M, Šumberová K, Hájková P, Hájek M, Hroudová Z, Navrátilová J, et al., editors. Vegetace České republiky - vodní a mokřadní vegetace. Prague: Academia; 2011. p. 100-247. (vol 3).
  • 4.Prausová R, Janová J. Současný stav výskytu rdestu dlouholistého (Potamogeton praelongus) v České republice. Příroda. 2010;27:155-168.
  • 5.Prausová R, Janová J, Adamec L. Rescue of the critically endangered long-stalked pondweed (Potamogeton praelongus) in the Czech Republic. Acta Biol Slov. 2011;54:43-54.
  • 6.Kitner M, Prausová R, Adamec L. Present status of genetic diversity of Potamogeton praelongus populations in the Czech Republic. Phyton. 2013;53(1):73-86.
  • 7.Waycott M. Assessment of genetic variation and clonality in the seagrass Posidonia australis using RAPD and allozyme analysis. Mar Ecol Prog Ser. 1995;116:289-295.
  • 8.Prausová R, Janová J, Šafářová, L. Testing achene germination of Potamogeton praelongus Wulfen. Cent Eur J Biol. 2013;8(1):78-86. http://dx.doi.org/10.2478/s11535-012-0114-4
  • 9.Ebert D, Haag C, Kirkpatrick M, Riek M, Hottinger JW, Pajunen VI. A selective advantage to immigrant genes in a Daphnia metapopulation. Science. 2002;295:485-488. http://dx.doi.org/10.1126/science.1067485
  • 10.Richards CM. Inbreeding depression and genetic rescue in a plant metapopulation. Am Nat. 2000;155:383-394.
  • 11.Saccheri IJ, Brakefield PM. Rapid spread of immigrant genomes into inbred populations. Proc R Soc Lond B. 2002;269:1073-1078. http:// dx.doi.org/10.1098/rspb.2002.1963
  • 12.Madsen T, Shine R, Olsson M, Wittzell H. Conservation biology: restoration of an inbred adder population. Nature. 1999;402:34-35. http://dx.doi.org/10.1038/46941
  • 13.Stokstad E. "Genetic rescue" helps panthers but puts researchers on the spot. Science. 2005;309:1162-1162. http://dx.doi.org/10.1126/ science.309.5738.1162 seasons. Current efforts in the frame of the rescue program for P. praelongus in the CR [29] aim to use the most recent findings about dormancy breaking in P. praelongus achenes [8] to gain genetically variable material for repatriations and re-introductions. The aim of this experiment was to find an alternative, possibly more efficient, method to rescue this critically endangered species in the CR and Central Europe.
  • 14.Westemeier RL, Brawn JD, Simpson SA, Esker TL, Jansen RW, Walk JW, et al. Tracking the long-term decline and recovery of an isolated population. Science. 1998;282:1695-1698. http://dx.doi.org/10.1126/ science.282.5394.1695
  • 15.Prausová R, Sikorová P, Šafářová L. Generative reproduction of long stalked pondweed (Potamogeton praelongus Wulfen) in the laboratory. Aquat Bot. 2014;120:268-274. http://dx.doi.org/10.1016/j. aquabot.2014.09.005
  • 16.Adamec L, Pásek K. Medium optimization for growing Aldrovanda vesiculosa in vitro. Carniv Plant Newslett. 2000; 29:122-124.
  • 17.Hintze, J. NCSS - Number Cruncher Statistical System. NCSS: Kaysville, UT; 2001.
  • 18.Hough RA, Wetzel RG. Photosynthetic pathways of some aquatic plants. Aquat Bot. 1977;3:297-313. http://dx.doi. org/10.1016/0304-3770(77)90035-3
  • 19.Husák Š, Kaplan Z. Studium a záchrana vybraných ohrožených druhů rodu Potamogeton. I. Potamogeton praelongus (Rameno u Stříbrného rybníka). Report for Agency for Nature Protection of the Czech Republic. Třeboň: Institute of Botany of the Academy of Science of the Czech Republic in Třeboň and Průhonice; 1997.
  • 20.Maberly SC, Spence DHN. Photosynthetic inorganic carbon use by freshwater plants. J Ecol. 1983;71:705-724.
  • 21.Berg C, Dengler J, Abdank A, Isermann M, editors. Die Pflanzengesellschaften Mecklenburg. Vorpommernsundihre Gefährdung - Textband. Jena: Weissdorn, Verlag; 2004.
  • 22.Burnett JH, editor. The vegetation of Scotland. Edinburgh: Oliver & Boyd; 1964.
  • 23.Doll R. Die Pflanzengesellschaften der stehender Gewässer in Mecklenburg - Vorpommern. Teil I. 3. Potamogetonetea Tx. et Prsg. 42. Laichkrautgesellschaften. Feddes Repert. 1991;102:217-317.
  • 24.Nowak A, Nowak S. Czerniawska-Kusza I. Rare and threatened pondweed communities in anthropogenic waterbodies of Opole Silesia (SW Poland). Acta Soc Bot Pol. 2007;76(2):151-163. http:// dx.doi.org/10.5586/asbp.2007.019
  • 25.Miljan A. Vegetations untersuchungen an Naturwiesen und Seen im Otepääschen Moränengebiet Estlands. Acta Commentat. 1933;25:1-139.
  • 26.Vöge M. Ecological studies on water plants of 14 sites around Kanger-lussuaq, southern West Greenland, with special regard to Potamogeton [Internet]. 2002 [cited 2014 Apr 23]. Available from: http://www. solo-tauchen.de/PDF/www04
  • 27.Fajer M, Waga JM, Rzetala M, Szymczyk A, Nita M, Machowski R, et al. The Late Vistulian and Holocene evolution of Jezioro Lake: a record of environmental change in southern Poland found in deposits and landforms. J Paleolimnol. 2012;48:651-667. http://dx.doi.org/10.1007/ s10933-012-9634-1
  • 28.Ellenberg H, Weber HE, Düll R, Wirth Y, Werner W, Paulißen D. Zeigerwerte der Gefässpflanzen Mitteleuropas. Scripta Geobot. 1991;18:1-248.
  • 29.Prausová R, Rybka V, Adamec L, Husák Š, Rydlo J. Záchranný program pro rdest dlouholistý. Depon. In: Report for Ministry of Environment of CR. Pardubice: Agency for Nature Protection of the Czech Republic; 2003.
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Bibliografia
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