Lowered oxygen concentration and culture in 3-dimension structure enhance immunological response of WJ-MSC to proinflammatory factors

• Autorzy: Figiel-Dabrowska A. , Lech W. , Zychowicz M. , Domanska-Janik K. , Sarnowska A.
• Języki publikacji: EN

Abstrakty

EN

INTRODUCTION: MSC-based therapy is becoming more and more common treatment of various diseases, albeit still as an experimental approach. According to present‑day literature, the therapeutic effects of transplanted cells would not be ascribed to their differentiation, trans‑differentiation or repopulation but rather to their paracrine effect on damaged tissue. This way of treatment can be initiated and enhanced by local environmental mediators. AIM(S): The aim of this study was to assess the influence of inflammation specific environment in vitro on secretory WJ-MSC properties and to evaluate the possibility of programmed and controlled induction and enhancement of anti‑inflammatory cell properties in the context of further cell therapy. METHOD(S): Our experiments were based on reconstruction in vitro the environment to which therapeutic cells (WJ‑MSC) are usually transplanted. The inflammatory conditions that occur around the transplant were reproduced through TNFα and IFNγ stimulation. Tissue specific oxygen concentration (5%), 3‑dimension transplant structure and chemical composition of the indirect transplanted cell surrounding as determined by additional scaffold ingredients (fibrin and platelet lysate) were also reconstituted. RESULTS: Carried experiments have shown specific changes in the secreted cytokine pallet induced in vitro by the inflammation‑like WJ‑MSC surrounding. We have proved that environmental modifications cause changes in synthesis and secretion of the determined proteins. Both, the physioxia introduced in our in vitro experiments and WJ cells cultured in 3-dimensional structures enhanced cytoprotective paracrine properties of WJ-MSC. Additional reinforcing effect was observed when therapeutic cells were transplanted on platelet lysate – containing scaffolds. CONCLUSIONS: Presented results indicate that by optimization of cell culture and transplantation conditions we could control and enhance cytokine-connected therapeutic properties of MSC. FINANCIAL SUPPORT: The work was supported by National Centre for Research and Development grant No Strategmed 1/234261/2/NCBR/2014.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2017
• Tom: 77
• Numer: Suppl.1
• Opis fizyczny: p.105

Twórcy

autor

Figiel-Dabrowska A.

• Stem Cell Bioengineering Unit, Mossakowski Medical Research Centre Polish Academy of Sciences, Warsaw, Poland

autor

Lech W.

• Stem Cell Bioengineering Unit, Mossakowski Medical Research Centre Polish Academy of Sciences, Warsaw, Poland

autor

Zychowicz M.

• Stem Cell Bioengineering Unit, Mossakowski Medical Research Centre Polish Academy of Sciences, Warsaw, Poland

autor

Domanska-Janik K.

• Stem Cell Bioengineering Unit, Mossakowski Medical Research Centre Polish Academy of Sciences, Warsaw, Poland

autor

Sarnowska A.

• Stem Cell Bioengineering Unit, Mossakowski Medical Research Centre Polish Academy of Sciences, Warsaw, Poland