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2018 | 67 | 1 |

Tytuł artykułu

Evaluation of the Carba NP test for the detection of carbapenemase activity in Bacteroides species

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
We evaluated the usefulness of the Carba NP test for rapid detection of carbapenemase activity in Bacteroides spp. The minimum inhibitory concentration (MIC) for imipenem was determined with gradient test strips, and cfiA gene was investigated by polymerase chain reaction for 27 clinical Bacteroides spp. isolates. Carba NP test was performed according to recommendations of the Clinical and Laboratory Standards Institute. Among three cfiA gene harboring clinical isolates, two imipenem resistant isolates were Carba NP test positive, while the imipenem intermediate isolate was negative. Our preliminary results suggest that the Carba NP test can be useful as a rapid test to detect carbapenemases in Bacteroides species.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

67

Numer

1

Opis fizyczny

p.97-101,fig.,ref.

Twórcy

autor
  • Department of Medical Microbiology, Acibadem University School of Medicine, Istanbul, Turkey
  • Acibadem Labmed Medical Laboratories, Department of Microbiology, Istanbul, Turkey
autor
  • Acibadem Labmed Medical Laboratories, Department of Microbiology, Istanbul, Turkey
autor
  • Department of Medical Microbiology, Acibadem University School of Medicine, Istanbul, Turkey
autor
  • Department of Medical Microbiology, Acibadem University School of Medicine, Istanbul, Turkey
  • Acibadem Labmed Medical Laboratories, Department of Microbiology, Istanbul, Turkey

Bibliografia

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  • Dortet L., L. Poirel, C. Errera and P. Nordmann. 2014. Carb Acineto NP test for rapid detection of carbapenemase-producingAcinetobacter spp. J. Clin. Microbiol. 52: 2359–2364.
  • Edwards R. and P.N. Read. 2000. Expression of ther carbapenemase gene (cfiA) in Bacteroides fragilis. J. Antimicrob. Chemother. 46(6): 1009–1012.
  • Eitel Z., J. Sóki, E. Urbán, E. Nagy and ESCMID Study Group on Anaerobic Infection. 2013. The prevalence of antibiotic resistance genes in Bacteroides fragilis group strains isolated in different European countries. Anaerobe. 21: 43–49.
  • EUCAST (European Committee on Antimicrobial Susceptibility Testing). 2016. Breakpoint tables for interpretation of MICs and zone diameters. Version 6.0. http://www.eucast.org.
  • Nagy E., E. Urbán and ESCMID Study Group on Antimicrobial Resistance in Anaerobic Bacteria. 2011. Antimicrobial susceptibility of Bacteroides fragilis group isolates in Europe: 20 years of experience. Clin. Microbiol. Infect. 17: 371–379.
  • Nordmann P., M. Gniadkowski, C.G. Giske, L. Poirel, N. Woodford, V. Miriagou and European Network on Carbapenemases. 2012. Identification and screening of carbapenemase-producing Enterobacteriaceae. Clin. Microbiol. Infect. 18: 432–438.
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  • Sóki J., E. Urbán, I. Szöke, E. Fodor and E. Nagy. 2000. Prevalence of the carbapenemase gene (cfiA) among clinical and normal flora isolates of Bacteroides species in Hungary. J. Med. Microbiol. 49: 427–430.
  • Thompson J.S. and M.H. Malamy. 1990. Sequencing the gene for an imipenem-cefoxitin-hydrolyzing enzyme (CfiA) from Bacteroides fragilis TAL2480 reveals strong similarity between CfiA and Bacillus cereus β-Lactamase II. J. Bacteriol. 172: 2584–2593.
  • Toprak N.U., O.D. Uzunkaya, J. Sóki and G. Soyletir. 2012. Susceptibility profiles and resistance genes for carbapenems (cfiA) and metronidazole (nim) among Bacteroides species in a Turkish University Hospital. Anaerobe.18: 169–171.
  • Wayne, P.A.; Clinical and Laboratory Standards Institute. 2016. CLSI Guidelines: Performance Standards for Antimicrobial Susceptibility Testing. 26th ed. CLSI supplements M100S.
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Typ dokumentu

Bibliografia

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Identyfikator YADDA

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