Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2009 | 31 | 2 |
Tytuł artykułu

Exogenus auxin regulates H2O2 metabolism in roots of tomato (Lycopersicon esculentum Mill.) seedlings affecting the expression and activity of CuZn-superoxide dismutase, catalase, and peroxidase

Treść / Zawartość
Warianty tytułu
Języki publikacji
The aim of this study was to test the effect of auxin treatment on selected parameters of the redox metabolism in roots. We found that auxin application results in a reduction in the H₂O₂ level in roots. The hormone stimulated CuZn-superoxide dismutase, but simultaneously increased the activities of catalase, cell wall bound ferulic acid peroxidase, and soluble peroxidase izoenzymes. The analysis of the expression of genes coding for the cytosolic izoform of CuZn-superoxide dismutase, catalase, and cell wall associated peroxidase (TPX 1) involved in cell wall stiffening and lignification revealed the stimulatory effect of exogenous auxin on the expression of the aforementioned genes. The enzyme activity and gene expression in the roots of control and auxin-treated plants were studied in daily intervals, during a 3-day-long growth cycle. The stimulatory effect of auxin on the enzymatic activity was transient with the highest stimulation observed on the second day of treatment. On the third day, the activities of the enzymes decreased. The maximal enzyme activities were preceded by a rise in gene expression. The increase in the level of CuZn-superoxide dismutase and catalase transcripts were detected after 1 day of auxin treatment. Then the expression of the aforementioned genes decreased. The period of auxin-dependent stimulation of the TPX 1 gene expression encompassed the first and the second day of treatment. Auxin stimulated CuZnsuperoxide dismutase and catalase activities only in the distal zone of the root while peroxidase activity was increased by auxin in the distal as well as in the proximal parts of the organ.
Słowa kluczowe
Opis fizyczny
  • Department of Biotechnology, Institute of General and Molecular Biology, Nicolaus Copernicus University, Gagarina 9, 87-100 Torun, Poland
  • Department of Biotechnology, Institute of General and Molecular Biology, Nicolaus Copernicus University, Gagarina 9, 87-100 Torun, Poland
  • Department of Biotechnology, Institute of General and Molecular Biology, Nicolaus Copernicus University, Gagarina 9, 87-100 Torun, Poland
  • Department of Biotechnology, Institute of General and Molecular Biology, Nicolaus Copernicus University, Gagarina 9, 87-100 Torun, Poland
  • Department of Biotechnology, Institute of General and Molecular Biology, Nicolaus Copernicus University, Gagarina 9, 87-100 Torun, Poland
  • Botella MA, Quesada MA, Hasegawa PM, Valpuesta V (1993) Nucleotide sequences of two peroxidase genes from tomato (Lycopersicon escdenfum). Plant Physiol 103:665–666. doi: 10.1104/pp.103.2.665
  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72:248–254. doi: 10.1016/0003-2697(76)90527-3
  • Brett CT, Waldron KW (1996) Physiology and biochemistry of plant cell walls, 2nd edn. Chapman & Hall, London, pp 46–51
  • Casimiro I, Marchant A, Bhalerao RP, Beeckman T, Dhooge S, Swarup R et al (2001) Auxin transport promotes Arabidopsis lateral root initiation. Plant Cell 13:843–852
  • Chadwick AV, Burg SP (1970) Regulation of root growth by auxin–ethylene interaction. Plant Physiol 45:192–200
  • Cleland RE (2004) Auxin and cell elongation. In: Davies PJ (ed) Plant hormones. Biosynthesis, signal transduction, action. Kluwer, Dordrecht, pp 204–220
  • Córdoba-Pedregosa MC, González-Reyes JA, Cañadillas MS, Navas P, Córdoba F (1996) Role of apoplastic and cell wall peroxidases on the stimulation of root elongation by ascorbate. Plant Physiol 112:1119–1125
  • Fry SC (1998) Oxidative scission of plant cell wall polyscchcarides by ascorbate-induced hydroxyl radicals. Biochem J 332:57–67
  • Hirt H (2000) Connecting oxidative stress, auxin, and cell cycle regulation through a plant mitogen-activated protein kinase pathway. Proc Natl Acad Sci USA 97:2405–2407. doi: 10.1073/pnas.97.6.2405
  • Hempel SL, Buettner GR, O’Malley YQ, Wessels DA, Flaherty DM (1999) Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: comparison with 2', 7'-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2', 7'-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123. Free Radic Biol Med 27:146–159. doi:10.1016/S0891-5849(99)00061-1
  • Jiang K, Meng YL, Feldman LJ (2003) Quiescent center formation in maize roots is associated with an auxin-regulated oxidizing environment. Development 130:1429–1438. doi:10.1242/dev. 00359
  • Joo JH, Bae YS, Lee JS (2001) Role of auxin-induced reactive oxygen species in root gravitropism. Plant Physiol 126:1055–1060
  • Karlsson M, Melzer M, Prokhorenko I, Johansson T, Wingsle G (2005) Hydrogen peroxide and expression of hipl-superoxide dismutase are associated with the development of secondary cell walls in Zinnia elegans. J Exp Bot 56:2085–2093. doi:10.1093/jxb/eri207
  • Kovtun Y, Chiu WL, Tena G, Sheen J (2000) Functional analysis of oxidative stress- activated mitogen-activated protein kinase cascade in plants. Proc Natl Acad Sci USA 97:2940–2945. doi: 10.1073/pnas.97.6.2940
  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685. doi:10.1038/227680a0
  • Lin CC, Kao CH (2001) Abscisic acid induced changes in cell wall peroxidase activity and hydrogen peroxide level in roots of rice seedlings. Plant Sci 160:323–329. doi:10.1016/S0168-9452(00) 00396-4
  • Liszkay A, Kenk B, Schopfer P (2003) Evidence for the involvement of cell wall peroxidase in the generation of hydroxyl radicals mediating extension growth. Planta 217:658–667. doi:10.1007/ s00425-003-1028-1
  • Liszkay A, van der Yalm E, Schopfer P (2004) Production of reactive oxygen intermediates (O₂-, H₂O₂ and ˙OH) by maize roots and their role in wall loosening and elongation growth. Plant Physiol 135:3114–3123. doi:10.1104/pp.104.044784
  • Luu-The V, Paquet N, Calvo E, Cumps J (2005) Improved real-time RT-PCR method for high-throughput measurements using second derivative calculation and double correction. Biotechniques 38:287–293
  • Medina MI, Quesada MA, Pliego F, Botella MA, Valpuesta V (1999) Expression of the tomato peroxidase gene TPX1 in NaCladapted and unadapted suspension cells. Plant Cell Rep 18:680–683. doi:10.1007/s002990050642
  • Mellersh DG, Foulds IV, Higgins VJ, Heath MC (2002) H₂O₂ plays different roles in determining penetration failure in three diverse plant–fungal interactions. Plant J 29:257–268. doi:10.1046/j. 0960-7412.2001.01215.x
  • Mittler R (2002) Oxidative stress, antioxidants, and stress tolerance. Trends Plant Sci 7:405–410. doi:10.1016/S1360-1385(02) 02312-9
  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:437–497. doi:10.1111/j.1399-3054.1962.tb08052.x
  • Ogawa K, Kanematsu S, Asada K (1997) Generation of superoxide anion and localization of CuZn-superoxide dismutase in the vascular tissue of spinach hypocotyls: their associacion with lignification. Plant Cell Physiol 38:1118–1126
  • Passardi F, Penel C, Dunand C (2004) Performing the paradoxical: how plant peroxidases modify the cell wall. Trends Plant Sci 9:534–540. doi:10.1016/j.tplants.2004.09.002
  • Pasternak T, Potters G, Caubergs R, Jansen MAK (2005) Complementary interactions between oxidative stress and auxin control plant growth responses at plant, organ, and cellular level. J Exp Bot 56:1991–2001
  • Pasternak TP, Őtvős K, Domoki M, Fehér A (2007) Linked activation of cell division and oxidative stress defense in alfalfa leaf protoplast-derived cells is dependent on exogenous auxin. Plant Growth Regul 51:109–117
  • Pfaffl MW (2001) A mathematical model for relative quantification in real-time PCR. Nucleic Acids Res 29:2002–2007. doi:10.1093/nar/29.9.e45
  • Potters G, Pasternak TP, Guisez Y, Palme KJ, Jansen MAK (2007) Stress-induced morphogenic responses: growing out of trouble? Trends Plant Sci 12:98–105
  • Quiroga M, Guerrero C, Botella MA, Barcelo A, Amaya I, Medina MI et al (2000) A tomato peroxidase involved in the synthesis of lignin and suberin. Plant Physiol 122:117–1127. doi:10.1104/pp.122.4.1119
  • Quiroga M, De Forchetti SM, Taleisnik E, Tigier H (2001) Tomato root peroxidase isoenzymes: kinetic studies of the coniferyl alcohol peroxidase activity, immunological properties and role in response to salt stress. J Plant Physiol 158:1007–1013. doi: 10.1078/0176-1617-00304
  • Rao MV, Paliyath G, Ormrod DP (1996) Ultraviolet B- and ozoneinduced biochemical changes in antioxidant enzymes of Arabidopsis thaliana. Plant Physiol 110:125–136. doi:10.1104/pp.110.1.125
  • Rodriguez AA, Grunberg KA, Taleisnik EL (2002) Reactive oxygen species in the elongation zone of maize leaves are necessary for leaf extension. Plant Physiol 129:1627–1632. doi:10.1104/pp. 001222
  • Romero-Romero T, Sánchez-Nieto S, SanJuan-Badillo A, Anaya AL, Cruz-Ortega R (2005) Comparative effects of allelochemical and water stress in roots of Lycopersicon esculentum Mill. (Solanaceae). Plant Sci 168:1059–1066. doi:10.1016/j.plantsci.2004. 12.002
  • Sanchez M, Pena MJ, Revilla G, Zarra I (1996) Changes in dehydroferulic acid and peroxidase activity against ferulic acid associated with cell walls during growth of Pinus pinaster hypocotyls. Plant Physiol 111:941–946
  • Schopfer P (2001) Hydroxyl radical-induced cell wall loosening in vitro and in vivo: implication for the control of elongation growth. Plant J 28:679–688. doi:10.1046/j.1365-313x.2001. 01187.x
  • Schopfer P, Liszkay A, Bechtold M, Frahry G, Wagner A (2002) Evidence that hydroxyl radicals mediate auxin-induced extension growth. Planta 214:821–828. doi:10.1007/s00425-001-0699-8
  • Tyburski J, Krzemiński Ł, Tretyn A (2008) Exogenous auxin affects ascorbate metabolism in roots of tomato seedlings. Plant Growth Regul 54:203–215
  • Veljovic-Jovanovic S, Noctor G, Foyer CH (2002) Are leaf hydrogen peroxide concentrations commonly overestimated? The potential influence of artefactual interference by tissue phenolics and ascorbate. Plant Physiol Biochem 40:501–507. doi:10.1016/ S0981-9428(02)01417-1
  • Zhang X, Zhang L, Dong F, Gao J, Galbraith DW, Song CP (2001) Hydrogen peroxide is involved in abscisic acid-induced stomatal closure in Vicia faba. Plant Physiol 126:1438–1448. doi: 10.1104/pp.126.4.1438
Rekord w opracowaniu
Typ dokumentu
Identyfikator YADDA
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.