Morphology of immune organs after very virulent plus strain of Marek’s disease virus infection in vaccinated hens

• Autorzy: Madej J.P. , Wozniakowski G. , Gawel A.
• Języki publikacji: EN

Abstrakty

EN

Marek’s disease (MD) outbreaks in poultry flocks may be associated with overriding of vaccine immune protection by very virulent (vvMDV) or very virulent plus (vv+MDV) strains. This paper presents the study on lymphoid organ morphology in the latent phase of MD caused by vv+MDV which break post-vaccinal protection in hens. We also immunohistochemically examined B and T populations as well as B/T and CD4+/CD8+ ratio of lymphocytes in lymphatic organs and, as a background, in MD lymphomas from non-lymphatic organs. The number of antigen expressed cells was evaluated as a percentage of positive cells in the one power field. Organ samples were collected from 24 dead reproductive hens (Ross 308 line) in age between 35-56 weeks, infected with vv+MDV. The hens originated from farms with MD outbreaks, despite earlier routine vaccination with CVI988/Rispens + HVT. The control organ samples originated from 15 clinically healthy hens at the same age and line, subjected to the same vaccination schedule. The number of CD3+, CD8+ and TCRγδ+ cells was significantly lower in MDV infected thymus, spleen and cecal tonsils in comparison to that found in the control organs. The proportion of CD4+ was also distinctly reduced in the thymus and limited in the spleen of MDV infected hens. This study revealed that infection with field vv+MDV isolates might break post-vaccinal protection and influence the central and peripheral immune system. The decrease in CD8+ and TCRγδ+ cell number in the thymus, spleen and cecal tonsils suggests that primarily these cells are involved in cell-mediated cytotoxicity against MDV transformed cells during latency.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2016
• Tom: 19
• Numer: 2
• Opis fizyczny: p.325-335,fig.,ref.

Twórcy

autor

Madej J.P.

• Department of Histology and Embryology, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Norwida 25, 50-375, Wroclaw, Poland

autor

Wozniakowski G.

• Department of Swine Diseases, National Veterinary Research Institute Pulawy (NVRI), al. Partyzantow 57, 24-100 Pulawy, Poland

autor

Gawel A.

• Department of Epizootiology and Clinic of Bird and Exotic Animals, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, pl. Grunwaldzki 45, 50-355 Wroclaw, Poland

Bibliografia

• Atkins KE, Read AF, Savill NJ, Renz KG, Islam AF, Walkden-Brown SW, Woolhouse ME (2013) Vaccination and reduced cohort duration can drive virulence evolution: Marek’s disease virus and industrialized agriculture. Evolution 67: 851-860.
• Baaten BJ, Butter C, Davison TF (2004) Study of host-pathogen interactions to identify sustainable vaccine strategies to Marek’s disease. Vet Immunol Immunopathol 100: 165-177.
• Baigent SJ, Smith LP, Nair VK, Currie RJ (2006) Vaccinal control of Marek’s disease: current challenges, and future strategies to maximize protection. Vet Immunol Immunopathol 112: 78-86.
• Burgess SC, Davison TF (1999) A quantitative duplex PCR technique for measuring amounts of cell-associated Marek’s disease virus: differences in two populations of lymphoma cells. J Virol Methods 82: 27-37.
• Davison F, Nair V (2005) Use of Marek’s disease vaccines: could they be driving the virus to increasing virulence? Expert Rev Vaccines 4: 77-88.
• Dudnikova E, Norkina S, Vlasov A, Slobodchuk A, Lee LF, Witter RL (2007) Evaluation of Marek’s disease field isolates by the „best fit” pathotyping assay. Avian Pathol. 36: 135-143.
• Haq K, Schat KA, Sharif S (2013) Immunity to Marek’s disease: where are we now? Dev Comp Immunol 41: 439-446.
• Heidari M, Fitzgerald SD, Zhang H (2014) Marek’s disease virus-induced transient cecal tonsil atrophy. Avian Dis 58: 262-270.
• Hunt HD, Dunn JR (2013) The influence of host genetics on Marek’s disease virus evolution. Avian Dis 57(2 Suppl): 474-482.
• Hunt HD, Dunn JR (2015) The Influence of Major Histocompatibility Complex and Vaccination with Turkey Herpesvirus on Marek’s Disease Virus Evolution. Avian Dis 59: 122-129.
• Igyarto BZ, Nagy N, Magyar A, Olhh I (2008) Identification of the avian B-cell-specific Bu-1 alloantigen by a novel monoclonal antibody. Poult Sci 87: 351-355.
• Kano R, Konnai S, Onuma M, Ohashi K (2009) Microarray analysis of host immune responses to Marek’s disease virus infection in vaccinated chickens. J Vet Med Sci 71: 603-610.
• Kodama H, Sugimoto C, Inage F, Mikami T (1979) Anti-viral immunity against Marek’s disease virus infected chicken kidney cells. Avian Pathol 8: 33-44.
• Mwangi WN, Smith LP, Baigent SJ, Beal RK, Nair V, Smith AL (2011) Clonal structure of rapid-onset MDV-driven CD4+ lymphomas and responding CD8+ T cells. PLoS Pathog 7: e1001337.
• Omar AR, Schat KA (1996) Syngeneic Marek’s disease virus (MDV)-specific cellmediated immune responses against immediate early, late, and unique MDV proteins. Virology 222: 87-99.
• Omar AR, Schat KA (1997) Characterization of Marek’s disease herpesvirus-specific cytotoxic T lymphocytes in chickens inoculated with a non-oncogenic vaccine strain of MDV. Immunology 90: 579-585.
• Osterrieder N, Kamil JP, Schumacher D, Tischer BK, Trapp S (2006) Marek’s disease virus: from miasma to model. Nat Rev Microbiol 4: 283-294.
• Pejović N, Velhner M, Polaček V, Aleksić-Kovačević S, Marinković D, Knežević M (2007) Morphological and immunohistochemical examination of tumor cells in Marek’s disease. Acta veterinaria (Beograd) 57: 27-35.
• Perumbakkam S, Hunt HD, Cheng HH (2014) Marek’s disease virus influences the core gut microbiome of the chicken during the early and late phases of viral replication. FEMS Microbiol Ecol 90: 300-312.
• Powell PC, Rennie M (1978) Marek’s disease tumour-specific antigen induced by the herpesvirus of turkeys in vaccinated chickens. Vet Record 103: 232-233.
• Ross LJN (1980) Mechanisms of protection conferred by HVT. In: Biggs PM (ed) Resistance and Immunity to Marek’s disease. Commission of the European Communities, Berlin, pp 289-297.
• Rothwell CJ, Vervelde L, Davison TF (1996) Identification of chicken Bu-1 alloantigens using the monoclonal antibody AV20. Vet Immunol Immunopathol 55: 225-234.
• Schat KA, Venugopal N (2008) Marek’s disease. In: Saif YM (ed) Diseases of Poultry, Blackwell Publishing Ltd, Iowa State Press, pp 452-514.
• Schat KA, Xing Z (2000) Specific and nonspecific immune responses to Marek’s disease virus. Dev Comp Immunol 24: 201-221.
• Venugopal K (2000) Marek’s disease: an update on oncogenic mechanisms and control. Res Vet Sci 69: 17-23.
• Witter RL (1996) Evolution of virulence of Marek’s disease virus: Evidence for a novel pathotype. In: Silva RF, Cheng HH, Coussens PM, Lee LF, Velicer LF (ed) Current Research on Marek’s Disease. American Association of Avian Pathologists, Inc., Kennett Square, PA, pp 86-91.
• Witter RL (1983) Characteristics of Marek’s disease viruses isolated from vaccinated commercial chicken flocks: association of viral pathotype with lymphoma frequency. Avian Dis 27: 113-132.
• Witter RL (1997) Increased virulence of Marek’s disease virus field isolates. Avian Dis 41: 149-163.
• Witter RL (2001) Protective efficacy of Marek’s disease vaccines. In: Hirai K (ed) Current topics in microbiology and immunology. Springer, Berlin, pp 58-90.
• Witter RL, Calnek BW, Buscaglia C, Gimeno IM, Schat KA (2005) Classification of Marek’s disease viruses according to pathotype: philosophy and methodology. Avian Pathol 34: 75-90.
• Witter RL, Lee LF, Fadly AM (1995) Characteristics of CVI988/Rispens and R2/23, two prototype vaccine strains of serotype 1 Marek’s disease virus. Avian Dis 39: 269-284.
• Witter RL, Gimeno IM (2006) Susceptibility of adult chickens, with and without prior vaccination, to challenge with Marek’s disease virus. Avian Dis 50: 354-365.
• Woźniakowski G, Samorek-Salamonowicz E (2014) Molecular evolution of Marek’s disease virus (MDV) field strains in a 40-year time period. Avian Dis 58: 550-557.
• Zhang YP, Li ZJ, Bao KY, Lv HC, Gao YL, Gao HL, Qi XL, Cui HY, Wang YQ, Ren XG, Wang XM, Liu CJ (2015) Pathogenic characteristics of Marek’s disease virus field strains prevalent in China and the effectiveness of existing vaccines against them. Vet Microbiol 177: 62-68.

Identyfikatory

DOI

10.1515/pjvs-2016-0040