PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2014 | 17 | 2 |
Tytuł artykułu

Supplementation of different concentrations of Orvus Es Paste (OEP) to ostrich egg yolk lipoprotein extender improves post-thaw boar semen quality

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
This study aimed to compare post-thaw quality of boar semen following freezing in an ostrich egg yolk lipoprotein (LPFo) extender supplemented with 0%, 0.25% and 0.50% Orvus Es Paste (OEP). Sperm assessments included total motility (TMOT), mitochondrial function (MF), plasma membrane integrity (PMI) and acrosome integrity (normal apical ridge, NAR). Considerable variations among boars and OEP treatments had a significant effect (P < 0.001) on post-thaw sperm characteristics. It was observed that post-thaw sperm characteristics were significantly compromised in semen samples frozen in the absence of OEP. By contrast, lactose-LPFo-glycerol extender supplemented with either 0.25% OEP or 0.50% OEP markedly enhanced post-thaw sperm characteristics. In all boars, there were no marked differences in post-thaw sperm TMOT between the freezing extenders supplemented with 0.25% and 0.50% OEP. However, a decline in the percentage of post-thaw motile spermatozoa was more pronounced in the extender supplemented with 0.50% OEP following a 120-min incubation period. Furthermore, the proportions of frozen-thawed spermatozoa with MF, PMI and NAR acrosomes varied significantly among the boars in the OEP-supplemented extenders. The findings of this study indicate that different OEP concentrations, in the presence of ostrich egg yolk lipoproteins, could have varying effects on post-thaw sperm survival.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
17
Numer
2
Opis fizyczny
p.225-230,fig.,ref.
Twórcy
autor
  • Department of Animal Biochemistry and Biotechnology, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn, Poland
autor
  • Department of Animal Biochemistry and Biotechnology, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn, Poland
autor
  • Department of Animal Biochemistry and Biotechnology, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn, Poland
Bibliografia
  • Aboagla EM, Terada T (2004) Effect of egg yolk during the freezing step of cryopreservation on the viability of goat spermatozoa. Theriogenology 62: 1160-1172.
  • Arriola J, Foote RH (1987) Glycerolation and thawing effects on bull spermatozoa frozen in detergent-treated egg yolk and whole egg extenders. J Dairy Sci 70: 1664-1670.
  • Axnér E, Hermansson U, Linde-Forsberg C (2004) The effect of Equex STM paste and sperm morphology on post-thaw survival of cat epididymal spermatozoa. Anim Reprod Sci 84: 179-191.
  • Cerolini S, Maldjian A, Pizzi F, Gliozzi TM (2001) Changes in sperm quality and lipid composition during cryopreservation of boar semen. Reproduction 121: 395-401.
  • Fraser L, Strzeżek J (2007) Effect of different procedures of ejaculate collection, extenders and packages on DNA integrity of boar spermatozoa following freezing-thawing. Anim Reprod Sci 99: 317-329.
  • Fraser L, Zasiadczyk Ł, Strzeżek J (2010) Interactions of egg yolk lipoprotein fraction with boar spermatozoa assessed with a fluorescent membrane probe. Folia Histochem Cytobiol 48: 292-298.
  • Garner DL, Johnson LA (1995) Viability assessment of mammalian sperm using SYBR-14 and propidium iodide. Biol Reprod 53: 276-284.
  • Kato S, Miyano T, Nanjo I, Yasui T, Kanada S (1990) Effect of concentration of sodium laurylsulfate on motility and acrosome morphology of frozen boar spermatozoa. Japan Journal of Animal Reproduction 36: 26-30.
  • Johnson LA, Weitze KF, Fiser P, Maxwell WM (2000) Storage of boar semen. Anim Reprod Sci 62: 143-172.
  • Moussa M, Martinet V, Trimeche A, Tainturier D, Anton M (2002) Low density lipoproteins extracted from hen egg yolk by an easy method: cryoprotective effect on frozen-thawed bull semen. Theriogenology 57: 1695-1706.
  • Niżański W, Klimowicz M, Partyka A, Savić M, Dubiel A (2009) Effects of the inclusion of Equex STM into Tris-based extender on the motility of dog spermatozoa incubated at 5oC. Reprod Domest Anim 44 (Suppl 2): 363-365.
  • Peña A, Linde-Forsberg C (2000) Effects of Equex, one- or two-step dilution, and two freezing and thawing rates on post-thaw survival of dog spermatozoa. Theriogenology 54: 859-875.
  • Pettit MJ, Buhr MM (1998) Extender components and surfactants affect boar sperm function and membrane behavior during cryopreservation. J Androl 19: 736-746.
  • Pillet E, Duchamp G, Batellier F, Beaumal V, Anton M, Desherces S, Schmitt E, Magistrini M (2011) Egg yolk plasma can replace egg yolk in stallion freezing extenders. Theriogenology 75: 105-114.
  • Pursel VG, Johnson LA, Schulman LL (1972) Interaction of extender composition and incubation period on cold shock susceptibility of boar spermatozoa. J Anim Sci 35: 580-584.
  • Pursel VG, Schulman LL, Johnson LA (1978) Effect of Orvus Es Paste on acrosome morphology, motility and fertilizing capacity of frozen-thawed boar sperm. J Anim Sci 47: 198-202.
  • Strzeżek J, Lecewicz L, Dziekońska A, Fraser L (2005) A simple method of extraction of lipoprotein fractions from avian egg yolk - protective effect on cooled boar semen. Theriogenology 63: 496-497.
  • Thomas CA, Garner DL, DeJarnette JM, Marshall CE (1998) Effect of cryopreservation on bovine sperm organelle function and viability as determined by flow cytometry. Biol Reprod 58: 786-793.
  • Waberski D, Weitze KF, Gleumes T, Schwarz M, Willmen T, Petzoldt R (1994) Effect of time of insemination relative to ovulation on fertility with liquid and frozen boar semen.Theriogenology 42: 831-840.
  • Watson PF (1975) Use of a Giemsa stain to detect changes in acrosomes of frozen ram spermatozoa. Vet Rec 97: 12-15.
  • Wu TW, Cheng FP, Chen IH, Yang CH, Tsai MY, Chang MH, Wang JH, Wu JT (2013) The combinatorial effect of different Equex STM Paste concentrations, cryoprotectants and the straw-freezing methods on the post-thaw boar semen quality. Reprod Domest Anim 48: 53-58.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-5c53e2ce-1453-4430-ad95-42b5fe7ac8e3
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.