Loop-mediated isothermal amplification as a simple molecular method for the detection of Derzsy’s disease virus

• Autorzy: Tarasiuk K. , Wozniakowski G. , Samorek-Salamonowicz E.
• Języki publikacji: EN

Abstrakty

EN

The objective of the study was to develop a simple and rapid molecular method for the detection of GPV. Twenty seven goose parvovirus (GPV) isolates collected from geese flocks in Poland were examined. Three pairs of specific primers: two outer primers (F3 and B3), two inner primers (FIP and BIP), and two loop primers (FL and BL) were used to accelerate the reaction. The optimum temperature and time of the reaction were 60°C and 30 min. The sensitivity of the method was 10-times higher than PCR. The method proved to be a sensitive, rapid, and specific assay for detecting GPV.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2013
• Tom: 57
• Numer: 1
• Opis fizyczny: p.19-23,fig.,ref.

Twórcy

autor

Tarasiuk K.

• Department of Poultry Viral Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Wozniakowski G.

• Department of Poultry Viral Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Samorek-Salamonowicz E.

• Department of Poultry Viral Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland

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Identyfikatory

DOI

10.2478/bvip-2013-0004