Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2018 | 40 | 01 |
Tytuł artykułu

SOC1 and AGL24 interact with AGL18-1, not the other family members AGL18-2 and AGL18-3 in Brassica juncea

Warianty tytułu
Języki publikacji
Many MCM1-AGAMOUS-DEFICIENS-SRF (MADS) genes have been proved to play an important role in the flowering time regulation of plants. The flowering-inhibiting factor AGAMOUS-LIKE 18 (AGL18) integrates into the two flowering-activating factors SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1) and AGAMOUS-LIKE 24 (AGL24), which play an important role during the plant developmental stages of the flowering pathway. However, it remains unknown whether and how the AGL18 protein directly interacts with SOC1 and/or AGL24 genes to regulate flowering time in Brassica juncea. In this study, three members (AGL18-1 in florescence, AGL18-2 and AGL18-3 in young seedlings) of the AGL18 family, and SOC1 and AGL24 in florescence were cloned in Brassica juncea. Yeast One-Hybrid assays and Dual-Glo® Luciferase assays showed that the SOC1 and AGL24 promoters interacted only with AGL18-1 protein, not AGL18-2 and AGL18-3. The typical conserved structure of the M-domain of AGL18-1 was the key region that mediated the interaction between the AGL18-1 protein and SOC1 promoter, and the I-domain, K-domain and C-domain did not regulate the interaction of AGL18-1/SOC1. In contrast, the K-domain and M-domain in AGL18-1 could mediate the interaction between the AGL18-1 protein and AGL24 promoter. This indicated that the AGL18-1 protein must have its unique functions that differed from AGL18-2 and AGL18-3. This work provides valuable information for in-depth studies into the molecular mechanisms of the AGL18 protein with SOC1 and AGL24 for flowering time control of Brassica juncea.
Słowa kluczowe
Opis fizyczny
Article 3 [11p.], fig.,ref.
  • College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China
  • College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China
  • Guizhou Province Horticulture Research Institute, Guiyang 550006, China
  • College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China
  • Bijie Station for Popularizing Agricultural Technique, Bijie 551700, Guizhou, China
  • College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China
  • College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China
  • College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China
  • College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China
  • Abe M, Kobayashi Y, Yamamoto S, Daimon Y, Yamaguchi A, Ikeda Y, Ichinoki H, Notaguchi M, Goto K, Araki T (2005) FD, a bZIP protein mediating signals from the floral pathway integrator FT at the shoot apex. Science 309(5737):1052–1056
  • Adamczyk Benjamin J, Lehti-Shiu Melissa D, Fernandez Donna E (2007) The MADS domain factors AGL15 and AGL18 act redundantly as repressors of the floral transition in Arabidopsis. Plant J 50(6):1007–1019
  • Alvarez-Buylla ER, Liljegren SJ, Pelaz S, Gold SE, Burgeff C, Ditta GS et al (2000) An ancestral MADS-box gene duplication occurred before the divergence of plants and animals. Proc Natl Acad Sci 97(10):5328–5333
  • Amasino R (2010) Seasonal and developmental timing of flowering. Plant J 61(6):1001–1013
  • Becker A, Theissen G (2003) The major clades of MADS-box genes and their role in the development and evolution of flowering plants. Mol Phylogenet Evol 29(3):464–489
  • Bell SP, Dutta A (2002) DNA replication in eukaryotic cells. Annu Rev Biochem 71:333–374
  • Borner R, Kampmann G, Chandler J, Gleissner R, Wisman E, Apel K, Melzer S (2000) A MADS domain gene involved in the transition to flowering in Arabidopsis. Plant J 24(5):591–599
  • Brass AL, Huang IC, Benita Y, John SP, Krishnan MN, Feeley EM, Ryan BJ, Weyer JL, van der Weyden L, Fikrig E, Adams DJ, Xavier RJ, Farzan M, Elledge SJ (2009) The IFITM proteins mediate cellular resistance to influenza A H1N1 virus, West Nile virus, and dengue virus. Cell 139(7):1243–1254
  • Coen ES, Meyerowitz EM (1991) The war of the whorls: genetic interactions controlling flower development. Nature 353(6339):31–37
  • Corbesier L, Vincent C, Jang S, Fornara F, Fan Q, Searle I, Giakountis A, Farrona S, Gissot L, Turnbull C, Coupland G (2007) FT protein movement contributes to long-distance signaling in floral induction of Arabidopsis. Science 316(5827):1030–1033
  • Davies B, EgeaCortines M, Silva ED, Saedler H, Sommer H (1996) Multiple interactions amongst floral homeotic MADS box proteins. EMBO J 15(16):4330–4343
  • Fan HY, Hu Y, Tudor M, Ma H (1997) Specific interactions between K domains of AG and AGLs, members of the MADS domain family of DNA binding proteins. Plant J 12(5):999–1010
  • Farnham PJ (2009) Insights from genomic profiling of transcription factors. Nat Rev Genet 10(9):605–616
  • Fernandez DE, Wang CT, Zheng YM, Adamczyk BJ, Singhal R, Hall PK, Perry SE (2014) The MADS-domain factors AGAMOUS-LIKE15 and AGAMOUS-LIKE18, along with SHORT VEGETATIVE PHASE and AGAMOUS-LIKE24, are necessary to block floral gene expression during the vegetative phase. Plant Physiol 165(4):1591–1603
  • Ferrandiz C, Liljegren SJ, Yanofsky MF (2000) Negative regulation of the SHATTERPROOF genes by FRUITFULL during Arabidopsis fruit development. Science 289(5478):436–438
  • Gu X, Wang Y, He Y (2013) Photoperiodic regulation of flowering time through periodic histone deacetylation of the florigen gene FT. PLoS Biol 11(9):e1001649
  • Hartmann U, Hohmann S, Nettesheim K, Wisman E, Saedler H, Huijser P (2000) Molecular cloning of SVP: a negative regulator of the floral transition in Arabidopsis. Plant J 21(4):351–360
  • Hellens RP, Allan AC, Friel EN, Bolitho K, Grafton K, Templeton MD, Karunairetnam S, Gleave AP, Laing WA (2005) Transient expression vectors for functional genomics, quantification of promoter activity and RNA silencing in plants. Plant Methods 1:13
  • Henschel K, Kofuji R, Hasebe M, Saedler H, Munster T, Theissen G (2002) Two ancient classes of MIKC-type MADS-box genes are present in the moss Physcomitrella patens. Mol Biol Evol 19(6):801–814
  • Higo K, Ugawa Y, Iwamoto M, Korenaga T (1999) Plant cis-acting regulatory DNA elements (PLACE) database. Nucleic Acids Res 27(1):297–300
  • Honma T, Goto K (2001) Complexes of MADS-box proteins are sufficient to convert leaves into floral organs. Nature 409(6819):525–529
  • Huang H, Tudor M, Weiss CA, Hu Y, Ma H (1995) The Arabidopsis MADS-box gene AGL3 is widely expressed and encodes a sequence-specific DNA-binding protein. Plant Mol Biol 28(3):549–567
  • Huang H, Tudor M, Su T, Zhang Y, Hu Y, Ma H (1996) DNA binding properties of two Arabidopsis MADS domain proteins: binding consensus and dimer formation. Plant Cell 8(1):81–94
  • Immink RGH, Posé D, Ferrario S, Ott F, Kaufmann K, Valentim FL, de Folter S, van der Wal F, van Dijk AD, Schmid M, Angenet GC (2012) Characterization of SOC1’s central role in flowering by the identification of its upstream and downstream regulators. Plant Physiol 160(1):433–449
  • Jack T (2001) Plant development going MADS. Plant Mol Biol 46(5):515–520
  • Jaeger KE, Wigge PA (2007) FT protein acts as a long-range signal in Arabidopsis. Curr Biol 17(12):1050–1054
  • Johansen B, Pedersen LB, Skipper M, Fredericksen S (2002) MADS-box gene evolution-structure and transcription patterns. Mol Phylogenet Evol 23(3):458–480
  • Kaufmann K, Melzer R, Theissen G (2005) MIKC-type MADS-domain proteins: structural modularity, protein interactions and network evolution in land plants. Gene 347(2):183–198
  • Klumpp K, Ruigrok RW, Baudin F (1997) Roles of the influenza virus polymerase and nucleoprotein in forming a functional RNP structure. The EMBO J 16(6):1248–1257
  • Kofuji Rumiko, Yamasaki Misuzu, Sumikawa Naomi, Kondo Kimihiko, Ueda Kunihiko, Ito Motomi, Hasebe Mitsuyasu (2003) Evolution and divergence of the MADS-box gene family based on genome-wide expression analyses. Mol Biol Evol 20(12):1963–1977
  • Lamb RS, Irish VF (2003) Functional divergence within the APETALA3/PISTILLATA floral homeotic gene lineages. Proc Natl Acad Sci 100(11):6558–6563
  • Lee JH, Yoo SJ, Park SH, Hwang I, Lee JS, Ahn JH (2007) Role of SVP in the control of flowering time by ambient temperature in Arabidopsis. Genes Dev 21(4):397–402
  • Lescot M, Dehais P, Thijs G, Marchal K, Moreau Y, Van de Peer Y, Rouze P, Rombauts S (2002) PlantCARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences. Nucleic Acids Res 30(1):325–327
  • Li D, Liu C, Shen L, Wu Y, Chen H, Robertson M, Helliwell CA, Ito T, Meyerowitz E, Yu H (2008) A repressor complex governs the integration of flowering signals in Arabidopsis. Dev Cell 15(1):110–120
  • Li T, Li QZ, Fan GL, Zuo YC, Peng Y (2013) PreDNA: accurate prediction of DNA-binding sites in proteins by integrating sequence and geometric structure information. Bioinformatics 6:678–685
  • Liu C, Zhou J, Bracha-Drori K, Yalovsky S, Ito T, Yu H (2007) Specification of Arabidopsis floral meristem identity by repression of flowering time genes. Development 134(10):1901–1910
  • Liu C, Chen H, Er HL, Soo HM, Kumar PP, Han JH, Liou YC, Yu H (2008) Direct interaction of AGL24 and SOC1 integrates flowering signals in Arabidopsis. Development 135(8):1481–1491
  • Liu L, Zhu Y, Shen LS, Yu H (2013) Emerging insights into florigen transport. Curr Opin Plant Biol 16(5):607–613
  • Mathieu J, Warthmann N, Küttner F, Schmid M (2007) Export of FT protein from phloem companion cells is sufficient for floral induction in Arabidopsis. Curr Biol 17(12):1055–1060
  • Melzer S, Lens F, Gennen J, Vanneste S, Rohde A, Beeckman T (2008) Flowering-time genes modulate meristem determinacy and growth form in Arabidopsis thaliana. Nat Genet 40(12):1489–1492
  • Michaels SD, Ditta G, Gustafson-Brown C, Pelaz S, Yanofsky M, Amasino RM (2003) AGL24 acts as a promoter of flowering in Arabidopsis and is positively regulated by vernalization. Plant J 33(5):867–874
  • Mizukami Y, Huang H, Tudor M, Hu Y, Ma H (1996) Functional domains of the floral regulator AGAMOUS: characterization of the DNA binding domain and analysis of dominant-negative mutations. Plant Cell 8(5):831–845
  • Moon YH, Jung JY, Kang HG, An GH (1999) Identification of a rice APETALA3 homologue by yeast two-hybrid screening. Plant Mol Biol 40(1):167–177
  • Moss T (2001) DNA-protein interactions: principles and protocols. Humana Press, Totowa
  • Nesi N, Debeaujon I, Jond C, Stewart AJ, Jenkins GI, Caboche M, Lepiniec L (2002) The TRANSPARENT TESTA16 locus encodes the ARABIDOPSIS BSISTER MADS domain protein and is required for proper development and pigmentation of the seed coat. Plant Cell 14(10):2463–2479
  • Notaguchi M, Abe M, Kimura T, Daimon Y, Kobayashi T, Yamaguchi A, Tomita Y, Dohi K, Mori M, Araki T (2008) Long-distance, graft-Transmissible action of Arabidopsis FLOWERING LOCUS T protein to promote flowering. Plant Cell Physiol 49(11):1645–1658
  • Pinyopich A, Ditta GS, Savidge B, Liljegren SJ, Baumann E, Wisman E, Yanofsky MF (2003) Assessing the redundancy of MADS-box genes during carpel and ovule development. Nature 424(6944):85–88
  • Pnueli L, Hareven D, Broday L, Hurwitz C, Lifsehitz E (1994) The TM5 MADS-box gene mediates organ differentiation in the three inner whorls of tomato flowers. Plant Cell 6(2):175–186
  • Riechmann JL, Meyerowitz EM (1997a) MADS domain proteins in plant development. Biol Chem 378(10):1079–1101
  • Riechmann JL, Meyerowitz EM (1997b) Determination of floral organ identity by Arabidopsis MADS domain homeotic proteins API, AP3, PI, and AG is independent of their DNA-binding specificity. Mol Biol Cell 8(7):1243–1259
  • Riechmann JL, Wang MQ, Meyerowitz EM (1996) DNA-binding properties of Arabidopsis MADS domain homeotic proteins APETALA1, APETALA3, PISTILLATA and AGAMOUS. Nucleic Acids Res 24(16):3134–3141
  • Samach A, Onouchi H, Gold SE, Ditta GS, Schwarz-Sommer Z, Yanofsky MF, Coupland G (2000) Distinct roles of CONSTANS target genes in reproductive development of Arabidopsis. Science 288(5471):1613–1616
  • Shore P, Sharrocks AD (1995) The MADS-box family of transcription factors. Eur J Biochem 229(1):1–13
  • Tao Z, Shen L, Liu C, Liu L, Yan Y, Yu H (2012) Genome-wide identification of SOC1 and SVP targets during the floral transition in Arabidopsis. Plant J 70(4):549–561
  • Theissen G, Kim JT, Saedler H (1996) Classification and phylogeny of the MADS-box multigene family suggest defined roles of MADS-box gene subfamilies in the morphological evolution of eukaryotes. J Mol Evol 43(5):484–516
  • Tiwari SB, Hagen G, Guifoyle TJ (2004) Aux/IAA proteins contain a potent transcriptional repression domain. Plant Cell 16(2):533–543
  • Verelst W, Twell D, de Folter S, Immink R, Saedler H, Munster T (2007) MADS-complexes regulate Transcriptome dynamics during pollen maturation. Genome Biol 8(11):R249
  • Wigge PA, Kim MC, Jaeger KE, Busch W, Schmid M, Lohmann JU, Weigel D (2005) Integration of spatial and temporal information during floral induction in Arabidopsis. Science 309(5737):1056–1059
  • Yanovsky MJ, Kay SA (2003) Living by the calendar: how plants know when to flower. Nat Rev Mol Cell Biol 4(4):265–276
  • Yoo SK, Wu X, Lee JS, Ahn JH (2011) AGAMOUSLIKE 6 is a floral promoter that negatively regulates the FLC/MAF clade genes and positively regulates FT in Arabidopsis. Plant J 65(1):62–76
  • Yu H, Xu Y, Tan EL, Kumar PP (2002) AGAMOVS-LIKE 24, a dosage-dependent mediator of the flowering signals. Proc Natl Acad Sci USA 99(25):16336–16341
  • Yu H, Ito T, Wellmer F, Meyerowitz EM (2004) Repression of AGAMOUSLIKE 24 is a crucial step in promoting flower development. Nat Genet 36(2):157–161
  • Zhang H, Forde BG (1998) An Arabidopsis MADS box gene that controls nutrient-induced changes in root architecture. Science 279(5349):407–409
  • Zheng Y, Ren N, Wang H, Stromberg AJ, Perry SE (2009) Global identification of targets of the Arabidopsis MADS domain protein AGAMOUSLike15. Plant Cell 21:2563–2577
Typ dokumentu
Identyfikator YADDA
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.