Determition of gentiopicroside contents during somatic embryogenesis in Gentiana straminea Maxim

• Autorzy: Yang L. , Wang H. , He T.
• Języki publikacji: EN

Abstrakty

EN

A protocol for regeneration of Gentiana straminea Maxim recently established in our laboratory, somatic embryogenesis was obtained from its leaf derived calli. The gentiopicroside contents of embryogenic calli, globular-, heart-, torpedo-, and cotyledon-shaped embryoids as well as regenerated plantlets were determined by high-performance liquid chromatography. Gentiopicroside was detectable in all materials tested. Embryogenic calli showed the lowest gentiopicroside content. The changes of gentiopicroside contents were not significant (P<0.05) with the development of somatic embryos. The highest gentiopicroside content (30.7 mg g⁻¹ dry weight) was achieved in regenerated plantlets. The contents of gentiopicroside were not significant (P<0.05) differences between control plants and embryogenic calli, different stages of somatic embryos and regenerated plantlets. This protocol could be employed for producing gentiopicroside or other medicinal compounds.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2014
• Tom: 36
• Numer: 07
• Opis fizyczny: p.1947-1951,fig.,ref.

Twórcy

autor

Yang L.

• School of Ecol-Environmental Engineering, Qinghai University, 810016 Xining, China
• State Key Laboratory Breeding Base for Innovation and Utilization of Plateau Crop Germplasm, Qinghai University, 810016 Xining, China

autor

Wang H.

• School of Ecol-Environmental Engineering, Qinghai University, 810016 Xining, China
• State Key Laboratory Breeding Base for Innovation and Utilization of Plateau Crop Germplasm, Qinghai University, 810016 Xining, China

autor

He T.

• School of Ecol-Environmental Engineering, Qinghai University, 810016 Xining, China
• State Key Laboratory Breeding Base for Innovation and Utilization of Plateau Crop Germplasm, Qinghai University, 810016 Xining, China

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Identyfikatory

DOI

10.1007/s11738-014-1557-1