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2012 | 68 | 02 |
Tytuł artykułu

Morphometrical analysis of neurons of corpus amygdaloideum's nucleus amygdaloideum lateralis in domestic pig

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The aim of the research was to conduct morphometrical analysis during the process of maturation of nucleus amygdaloideum lateralis. The brain of domestic pigs of both sexes (taken during the following period of their life: from 7th to 15th week of intrauterine life, newborn animals, one-month and one-year-old animals) were used as the material for the examination. The brains were removed and processed conventionally by microscope. The preparations were colored according to Klüvera-Barrer’s method and according to Nissel. Histological preparations obtained in this way were used for morphometrical analysis of the neurons of the corpus amygdaloideum’s nucleus amygdaloideum lateralis. Morphometrical examinations were carried out by the Nikon Eclipse E-600 microscope compressed with a JVC TK-1380-E camera and a computer using morphometrical MULTI-SCAN-BASE 08.98 program. The examination comprised the following parameters: the section area of nervous cells and the area of cell nucleus in µm²; the nucleo-cellar rate in %; the average diameter of nervous cell in µm; the volume of nervous cells in µm³; the number of neurons per 1 mm²; the number of neurons per 1 mm³. Morphometrical observation showed that in the 9th week of fetal life the cells forming the primary corpus amygdaloideum are of identical shape and size. The size of cell area of this period of fetal life fluctuates around 45 µm². In the 11th week of fetal life cells of the nucleus amygdaloideum lateralis are densely arranged, they have oval or polyhedral shape and contain a small amount of cytoplasm pretty evenly stained, in which Nissel’s granules are visible. In the 12th week of a domestic pig’s life cells of the nucleus amygdaloideum lateralis have a polyhedral, oval or pyramidal shape. In the subsequent periods of fetal life and new born animals, the appearance of cells composing the nucleus amygdaloideum laterais does not significantly change. In case of nucleus amygdaloideum lateralis nucleo-cellular rate fluctuates between 51 and 60%. The value of this rate decreases during development and maturation of neurons. On the basis of the examined morphometrical parameters the author claims that the neurons attain morphological maturity at the end of fetal life.
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  • Department of Animal Anatomy and Histology, SubDepartment of Animal Anatomy, University of Life Sciences in Lublin, ul. Akademicka 12, 20-950 Lublin
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