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2015 | 14 | 3 |
Tytuł artykułu

Lactobacillus strains belonging to Casei group display various adherence to enterocytes and mucus

Treść / Zawartość
Warianty tytułu
PL
Zróżnicowanie zdolności bakterii z rodzaju Lactobacillus należących do grupy Casei do przylegania do enterocytów i śluzu przewodu pokarmowego
Języki publikacji
EN
Abstrakty
EN
Background. The ability of lactobacilli to adhere to the surface of the intestine is an important functional characteristic which can largely determine the effective colonization of the intestinal tract by probiotics. The following study compares the adhesion efficiency of the twenty strains of Lactobacillus genus belonging to Casei group to the Caco-2 cells and gastrointestinal mucus. Material and methods. Twenty isolates of lactobacilli belonging to Casei group were tested. The ability of bacterial cells to adhere to mucus was examined using adhesion assay to gastrointestinal mucus. Obtained results were compared with adhesion efficiency to Caco-2 cells. Phylogenetic relationship between isolates was analysed by rep-PCR. Results. The results showed large differences in adhesion efficiency between strains, as well as differences in the efficiency of adhesion to the intestinal epithelial cells and mucus. Group similarity highlighted by a rep- PCR technique does not correspond with groups of similarity in terms of the characteristics of the ability to adhere to mucus or the epithelial cells of intestinal tract. Conclusions. Strains having a high adhesion efficiency to enterocytes do not always show a high adhesion efficiency to the mucus. This may indicate the presence of different and multiple factors responsible for adhesion efficiency of Lactobacillus group Casei strains to epithelial cells and mucus.
PL
Zdolność bakterii Lactobacillus z grupy Case do przylegania do powierzchni jelit jest istotną cechą funkcjonalną, która w znacznym stopniu może decydować o skuteczności probiotyku. W proces adhezji są zaangażowane liczne czynniki obecne na powierzchni komórek bakteryjnych oraz nabłonka jelitowego. Złożony charakter zjawiska adhezji sprawia, że dokładne poznanie jego mechanizmów jest trudne i wymaga licznych badań. W pracy porównano wydajność adhezji dwudziestu szczepów bakterii Lactobacillus z grupy Casei do komórek Caco-2 oraz do śluzu przewodu pokarmowego. Uzyskane wyniki wykazały duże zróżnicowanie wydajności adhezji między poszczególnymi szczepami, jak również różnice w wydajności adhezji bakterii do komórek nabłonkowych jelit i śluzu.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
14
Numer
3
Opis fizyczny
p.247-255,fig.,ref.
Twórcy
autor
  • Department of Food Biotechnology and Microbiology, Poznan University of Life Sciences, Wojska Polskiego 28, 60-637 Poznan, Poland
autor
  • Department of Food Biotechnology and Microbiology, Poznan University of Life Sciences, Wojska Polskiego 28, 60-637 Poznan, Poland
Bibliografia
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  • Darilmazl, D. O., Aslim, B., Suludere, Z., Akca, G. (2011). Influence of gastrointestinal system conditions on adhesion of exopolysaccharide-producing Lactobacillus delbrueckii subsp. bulgaricus strains to Caco-2 Cells. Braz. Arch. Biol. Technol., 54, 5, 917-926.
  • Douillard, F. P., Ribbera, A., Järvinen, H. M., Kant, R., Pietilä, T. E., Randazzo, C., ..., de Vos, W. M. (2013). Comparative genomic and functional analysis of Lactobacillus casei and Lactobacillus rhamnosus strains marketed as probiotics. Appl. Environ. Microbiol., 79, 6, 1923-1933.
  • Douillard, F. R, Ribbera, A., Kant, R., Pietila, T. E., Jarvin- en, H. M., Messing, M.,..., de Vos, W. M. (2013). Comparative genomic and functional analysis of 100 Lactobacillus rhamnosus strains and their comparison with strain GG. PLOS Genet., 9, 8, 1-15.
  • Felis, G. E., Dellaglio, F. (2007). Taxonomy of lactobacilli and bifidobacteria. Curr. Issues Intest. Microbiol., 8, 2, 44-61.
  • Grangette, C., Nutten, S., Palumbo, E., Morath, S., Hermann, C., Dewulf, J., ..., Mercenier, A. (2005). Enhanced anti-inflammatory capacity of a Lactobacillus plantarum mutant synthesizing modified teichoic acids. Proc. Natl. Acad. Sei. USA, 102, 29, 10321-10326.
  • Guidelines for the evaluation of probiotics in food. Report of a Joint FAO/WHO Working Group on Drafting Guidelines for the Evaluation of Probiotics in Food. (2002). London, Ontario, Canada.
  • Hisbergues, M., Magi, M., Rigaux, R, Steuve, J., Garcia, L., Goudercourt, D., ..., Jacquet, A. (2007). In vivo and in vitro immunomodulation of Der p 1 allergen-specific response by Lactobacillus plantarum bacteria. Clin. Exp. Allergy, 37, 9, 1286-1295.
  • Kankainen, M., Paulina, L., Tynkkynenb, S., Von Ossows- kic, L, Reunanenc, J., Partanena, P., ..., De Vos, W. M. (2009). Comparative genomic analysis of Lactobacillus rhamnosus GG reveals pili containing a human mucus binding protein. PNAS, 106, 40, 17193-17198.
  • Landersjo, C., Yang, Z., Huttunen, E., Widmalm, G. (2002). Structural studies of the exopolysaccharides produced by Lactobacillus rhamnosus strain GG (ATCC 53103). Biomacromolecules, 3, 4, 880-884.
  • Maniatis, T., Jeffrey, A., van deSande, H. (1975). Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis. Biochemistry, 14, 17, 3787-3794.
  • Markowicz, C., Olejnik-Schmidt, A., Borkowska, M., Schmidt, M. T. (2014). SpaCBA sequence instability and its relationship to the adhesion efficiency of Lactobacillus casei group isolates to Caco-2 cells. Acta Biochim. Pol., 61, 2, 341-347.
  • Rasinkangas, R, Reunanen, J., Douillard, F. R, Ritari, J., Uotinen, V, Palva, A., de Vos, W. M. (2014). Genomic characterization of non-mucus adherent derivatives of Lactobacillus rhamnosus GG reveals genes affecting pilus biogenesis. Appl. Environ. Microbiol., 80, 22, 7001-7009.
  • Reunanen, J., von Ossowski, L, Hendrickx, A. P. A., Palva, A., de Vos, W. M. (2012). Characterization of the spaC- B A pilus fibers in the probiotic Lactobacillus rhamnosus GG. App. Environ. Microbiol., 78, 7, 2337-2344.
  • Roos, S., Jonsson, H. (2002). A high-molecular-mass cell- surface protein from Lactobacillus reuteri 1063 adheres to mucus components. Microbiology, 148, 2, 433-442.
  • Ruas-Madiedo, R, Gueimonde, M., Margolles, A., de los Reyes-Gavilan, C. G., Salminen, S. (2006). Exopolysaccharides produced by probiotic strain modify the adhesion of probiotics and enteropathogens to human intestinal mucus. J. Food Prot., 69, 8, 2011-2015.
  • Schmidt, M. T., Olejnik-Schmidt, A. K., Myszka, K. Borkowska, M., Grajek, W. (2010). Evaluation of quantitative PCR measurement of bacterial colonization of epithelial cells. Pol. J. Microbiol., 59, 2, 83-93.
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Typ dokumentu
Bibliografia
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Identyfikator YADDA
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