The influence of doxazosin on the contractility of the urinary bladder in female pigs with experimentally induced cystitis

• Autorzy: Markiewicz W. , Bossowska A. , Lepiarczyk E. , Majewska M. , Radziszewski P. , Wisniewska A. , Jaroszewski J.J.
• Języki publikacji: EN

Abstrakty

EN

The present in vitro study investigated the influence of doxazosin on the contractility of the urinary bladder in female pigs with experimentally induced cystitis. Fifteen juvenile female piglets (18-20 kg body weight) were randomly assigned into three groups (n=5 animals each): i) control (clinically healthy animals, without doxazosin treatment), ii) animals with induced inflammation of the urinary bladder, but without doxazosin treatment (experimental group I) and iii) animals with inflamed bladder, treated orally with doxazosin (0.1 mg/kg body weight for 30 days; experimental group II). Thereafter, the pigs were sacrificed and strips of the bladder trigone were suspended in organ baths. The tension and amplitude of the smooth muscles was measured before and after exposition to 5-hydroxytryptamine (5-HT; 10⁻⁶-10⁻⁴ M), acetylocholine (ACh; 10⁻⁵-10⁻³ M) and norepinephrine (NE; 10⁻⁹-10⁻⁷ M). 5-HT caused an increase in the tension of contractions in all the groups and the amplitude in the experimental groups, however, the effect was higher in the experimental group I than in group II as compared to that found in the pre-treatment period. ACh caused an increase in the tension in the control group and a decrease in the amplitude in both experimental groups; these changes significantly differed between the control and doxazosin-treated group. NE caused a decrease in the tension in both experimental groups and amplitude in all the groups, however, the effect was most strongly expressed in doxazosine-treated group. The present study has revealed that long-term administration of doxazosin causes a desensitization of the detrusor smooth muscle to in vitro applied mediators in the autonomic nervous system.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2017
• Tom: 20
• Numer: 3
• Opis fizyczny: p.485-490,fig.,ref.

Twórcy

autor

Markiewicz W.

• Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 13, 10-718 Olsztyn, Poland

autor

Bossowska A.

• Department of Human Physiology, Faculty of Medical Sciences, University of Warmia and Mazury, Warszawska 30, 10-082 Olsztyn, Poland

autor

Lepiarczyk E.

• Department of Human Physiology, Faculty of Medical Sciences, University of Warmia and Mazury, Warszawska 30, 10-082 Olsztyn, Poland

autor

Majewska M.

• Department of Human Physiology, Faculty of Medical Sciences, University of Warmia and Mazury, Warszawska 30, 10-082 Olsztyn, Poland

autor

Radziszewski P.

• Department and Clinic of Urology, Faculty of Medicine, Warsaw Medical University, Lindley’a 4, Warsaw, Poland

autor

Wisniewska A.

• Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 13, 10-718 Olsztyn, Poland

autor

Jaroszewski J.J.

• Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 13, 10-718 Olsztyn, Poland

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Identyfikatory

DOI

10.1515/pjvs-2017-0058