Impact of probiotic on the number of lactic acid rod s forming hydrogen peroxide isolated from porkers and on changes in drug resistance of selected Escherichia coli isolates

• Autorzy: Selwet M. , Galbas M. , Dullin P.
• Języki publikacji: EN

Abstrakty

EN

The presented investigations were conducted on a group of 60 porkers of crossbreed Polish Landrace x Large White Polish. The animals were divided into two equal experimental groups. The control group (K) was fed diets without supplementation with probiotics, group (P) - diets with the addition of probiotic (0.2 kg t⁻¹ feed). The aim of the study was to determine the effect of probiotic preparation on total numberof lactic acid rods from the Lactobacillus genus and those forming hydrogen oxide. The second part of experiment concerned the influence of probiotic preparation on the number, haemolytic ability and changes in drug resistance of Escherichia coli isolated from animal faeces. The significantly highest number of Lactobacillus sp. were determined in the saliva of porkers fed diets with the addition of probiotic, while the lowest in the control group. Lactobacillus sp. rods capable of forming hydrogen peroxide were isolated from 17 animals in group K and from three animals in group P. E. coli was determined in each examined sample of faeces. In groups K and P, counts of these bacteria were similar and did not differ statistically. High numbers of haemolytic isolates (haemolysis ß) were found in faeces of animals fed diets with the addition of probiotic. Number and proportions of resistant isolates in groups K and P were different. Gentamicin was characterised by exceptionally high in vitro effectiveness. The used probiotic increased drug resistance of E. coli and increased frequency of incidence of haemolysis ß.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2012
• Tom: 56
• Numer: 1
• Opis fizyczny: p.21-25,ref.

Twórcy

autor

Selwet M.

• Department of General and Environmental Microbiology, Poznan University of Life Sciences, 60-656 Poznan, Poland

autor

Galbas M.

autor

Dullin P.

Bibliografia

• 1. Adesokan I.A., Ekanola Y.A., Okanlawon B.M.: Influence of cultural conditions on hydrogen peroxide production by lactic acid bacteria isolated from some Nigerian traditional fermented foods. Afr J Microbiol Res 2010, 4, 1991-1996.
• 2. Brashears M.M., Jaroni D., Trimble J.: Isolation, selection and characterization of lactic acid bacteria from competitive exclusion product to reduce shedding of Escherichia coli O157:H7 in cattle. J Food Protect 2003, 66, 355-363.
• 3. Casey P.G., Casey G.D., Gardiner G.E., Tangney M., Stanton C., Ross R.P., Hill C., Fitzgerald G.F.: Isolation and chrakterization of anti-Salmonella lactic acid bakteria from the porcine gastrointestinal tract. Letters Appl Microbiol 2004, 39, 431-438.
• 4. Cao L.T., Wu J.Q., Xie F., Hu S.H., Mo Y.: Efficacy of nisin in treatment of clinical mastitis in lactating dairy cows. J Dairy Sci 2007, 90, 3980-3985.
• 5. Dacko J., Osek J.: Analysis of selected phenotype properties of Escherichia coli strains isolated from piglet. Medycyna Wet 2004, 60, 861-866.
• 6. Estienne M.J., Hartsock T.G., Harper A.F.: Effects of antibiotics and probiotics on suckling pig and weaned pig performance. Int J Appl Res Vet Med 2005, 3, 303- 308.
• 7. Ito A., Sato Y., Kudo S., Sato S., Nakajima H., Toba T.: The screening of hydrogen peroxide producing lactic acid bacteria and their application to inactivating psychotrophic food borne pathogens. Curr Microbiol 2003, 47, 231-236.
• 8. Janik A., Koska M., Paluch U., Pieszka M., Barowicz T.: Probiotics in piglet nutrition. Wiad Zootech 2006, 1, 3-9.
• 9. Klebanoff S.J., Hillier S.L., Eschenbach D.A., Waltersdorph A.M.: Control of microbial flora of the vagina by H₂O₂-generating lactobacilli. J Infect Dis 1991, 164, 94-100.
• 10. Mazur E., Klag S.: Mechanisms of antimicrobial resistance. Med Rodzinna 2004, 6, 278-281.
• 11. Ogunbanawo S.T.: Functional properties of lactic acid bacteria isolated from ogi and fufu, two Nigerian fermented foods. Adv Food Sci 2005, 27, 14-21.
• 12. Osek J.: Drug resistance of Escherichia coli strains of O157:H7 group isolated from humans and animals. Medycyna Wet 2003, 59, 993-996.
• 13. Patterson J.L., Girerd P.H., Karjane N.W., Jefferson K.K.: Effect of biofilm phenotype on resistance of Gardnerella vaginalis to hydrogen peroxide and lactic acid. Am J Obstet Gynecol 2008, 170, 171-177.
• 14. Pericone C.D., Overweg K., Hermans P.W., Weiser J.N.: Inhibitory and bactericidal effects of hxdrogen peroxide production by Streptococcus pneumoniae on other inhabitants of upper respiratory tract. Infect Immun 2000, 68, 3990-3997.
• 15. Rekiel A.: Effect of probiotic on the biochemical parameters in blood of fatteners. Medycyna Wet 2008, 64, 110-112.
• 16. SAS.: SAS User's quide. Statistics version, 1999, SAS Institute Inc., Cary, North Carolina.
• 17. Selwet M., Galbas M., Porzucek F.: Drug-resistance of Escherichia coli strains isolated from piglets to some antibiotics. Nauka Przyr Technol 2010, 4, 1-8
• 18. Sookkhee S., Chulasiri M., Prachyabrued A.: Lactic acid bacteria from healthy oral cavitx of Thai volunteers: inhibition of oral pathogens. J Appl Microbiol 2001, 90, 172-179.
• 19. Śliżewska K., Biernasik J., Libudzisz Z.: Probiotics as alternative for antibiotics. Sci Bull Lodz Techn Univ Food Chem Biotechnol 2006, 70, 79-91.
• 20. Truszczyński M., Pejsak Z.: Possibilities for compensating the negative effects of the ban of antimicrobial growth promoters in swine. Medycyna Wet 2007, 63, 10-13.
• 21. Zalán Z., Németh E., Baráth A., Halász A.: Influence of growth medium on hydrogen peroxide and bacteriocin production of Lactobacillus strains. Food Technol Biotechnol 2005, 3, 219-225.