PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2014 | 19 | 3 |

Tytuł artykułu

CD39/NTPDase-1 expression and activity in human umbilical vein endothelial cells are differentially regulated by leaf extracts from Rubus caesius and Rubus idaeus

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Many experimental studies have demonstrated the favorable biological activities of plants belonging to the genus Rubus, but little is known of the role of Rubus leaf extracts in the modulation of the surface membrane expression and activity of endothelial apyrase. The aim of this study was to assess the influence of 1–15 μg/ml Rubus extracts on CD39 expression and enzymatic activity, and on the activation (ICAM-1 expression) and viability of human umbilical vein endothelial cells (HUVEC). The polyphenolic contents and antioxidative capacities of extracts from dewberry (R. caesius L.) and raspberry (R. idaeus L.) leaves were also investigated. The techniques applied were flow cytometry (endothelial surface membrane expression of ICAM-1 and CD39), malachite green assay (CD39 activity), HPLC-DAD (quantitative analysis of polyphenolic extract), ABTS, DPPH and FRAP spectrometric assays (antioxidant capacity), and the MTT test (cell viability). Significantly increased CD39 expressions and significantly decreased ATPDase activities were found in the cells treated with 15 μg/ml of either extract compared to the results for the controls. Neither of the extracts affected cell proliferation, but both significantly augmented endothelial cell ICAM-1 expression. The overall antioxidant capacities of the examined extracts remained relatively high and corresponded well to the determined total polyphenol contents. Overall, the results indicate that under in vitro conditions dewberry and raspberry leaf extracts have unfavorable impact on endothelial cells.

Wydawca

-

Rocznik

Tom

19

Numer

3

Opis fizyczny

p.361-380,fig.,ref.

Twórcy

autor
  • Department of Haemostasis and Haemostatic Disorders, Medical University of Lodz, Lodz, Poland
autor
  • Department of Haemostasis and Haemostatic Disorders, Medical University of Lodz, Lodz, Poland
autor
  • Department of Haemostasis and Haemostatic Disorders, Medical University of Lodz, Lodz, Poland
autor
  • Department of Haemostasis and Haemostatic Disorders, Medical University of Lodz, Lodz, Poland
autor
  • Department of Biotechnology and Food Sciences, Lodz University of Technology, Lodz, Poland
autor
  • Department of Biotechnology and Food Sciences, Lodz University of Technology, Lodz, Poland
autor
  • Department of Haemostasis and Haemostatic Disorders, Medical University of Lodz, Lodz, Poland

Bibliografia

  • 1. Marcus, A.J., Safier, L.B., Broekman, M.J., Islam, N., Fliessbach, J.H., Hajjar, K.A., Kaminski, W.E., Jendraschak, E., Silverstein, R.L. and von Schacky, C. Thrombosis and inflammation as multicellular processes: significance of cell-cell interactions. Thromb. Haemost. 74 (1995) 213–217.
  • 2. Preissner, K.T. Anticoagulant potential of endothelial cell membrane components. Haemostasis 18 (1988) 271–300.
  • 3. Marcus, A.J., Safier, L.B., Hajjar, K.A., Ullman, H.L., Islam, N., Broekman, M.J. and Eiroa, A.M. Inhibition of platelet function by an aspirin-insensitive endothelial cell ADPase. Thromboregulation by endothelial cells. J Clin. Invest. 88 (1991) 1690–1696.
  • 4. Bakker, W.W., Poelstra, K., Barradas, M.A. and Mikhailidis, D.P. Platelets and ectonucleotidases. Platelets 5 (1994) 121–129.
  • 5. Marcus, A.J. and Safier, L.B. Thromboregulation: multicellular modulation of platelet reactivity in hemostasis and thrombosis. FASEB J. 7 (1993) 516–522.
  • 6. Plesner, L. Ecto-ATPases: identities and functions. Int. Rev. Cytol. 158 (1995) 141–214.
  • 7. Robson, S.C., Kaczmarek, E., Siegel, J.B., Candinas, D., Koziak, K., Millan, M., Hancock, W.W. and Bach, F.H. Loss of ATP diphosphohydrolase activity with endothelial cell activation. J. Exp. Med. 185 (1997) 153–163.
  • 8. Garbacki, N., Kinet, M., Nusgens, B., Desmecht, D. and Damas, J. Proanthocyanidins, from Ribes nigrum leaves, reduce endothelial adhesion molecules ICAM-1 and VCAM-1. J. Inflamm. (Lond). 2 (2005) 9.
  • 9. Leeuwenberg, J.F., Smeets, E.F., Neefjes, J.J., Shaffer, M.A., Cinek, T., Jeunhomme, T.M., Ahern, T.J. and Buurman, W.A. E-selectin and intercellular adhesion molecule-1 are released by activated human endothelial cells in vitro. Immunology 77 (1992) 543–549.
  • 10. Kim, J.H., Auger, C., Kurita, I., Anselm, E., Rivoarilala, L.O., Lee, H.J., Lee, K.W. and Schini-Kerth, V.B. Aronia melanocarpa juice, a rich source of polyphenols, induces endothelium-dependent relaxations in porcine coronary arteries via the redox-sensitive activation of endothelial nitric oxide synthase. Nitric Oxide 35C (2013) 54–64.
  • 11. Torres-Urrutia, C., Guzman, L., Schmeda-Hirschmann, G., Moore-Carrasco, R., Alarcon, M., Astudillo, L., Gutierrez, M., Carrasco, G., Yuri, J.A., Aranda, E. and Palomo, I. Antiplatelet, anticoagulant, and fibrinolytic activity in vitro of extracts from selected fruits and vegetables. Blood Coagul. Fibrinolysis 22 (2011) 197–205.
  • 12. Suh, J.H., Romain, C., Gonzalez-Barrio, R., Cristol, J.P., Teissedre, P.L., Crozier, A. and Rouanet, J.M. Raspberry juice consumption, oxidative stress and reduction of atherosclerosis risk factors in hypercholesterolemic golden Syrian hamsters. Food Funct. 2 (2011) 400–405.
  • 13. Liu, Z., Schwimer, J., Liu, D., Lewis, J., Greenway, F.L., York, D.A. and Woltering, E.A. Gallic acid is partially responsible for the antiangiogenic activities of Rubus leaf extract. Phytother. Res. 20 (2006) 806–813.
  • 14. Rojas-Vera, J., Patel, A.V. and Dacke, C.G. Relaxant activity of raspberry (Rubus idaeus) leaf extract in guinea-pig ileum in vitro. Phytother. Res. 16 (2002) 665–668.
  • 15. Durgo, K., Belscak-Cvitanovic, A., Stancic, A., Franekic, J. and Komes, D. The bioactive potential of red raspberry (Rubus idaeus L.) leaves in exhibiting cytotoxic and cytoprotective activity on human laryngeal carcinoma and colon adenocarcinoma. J. Med. Food 15 (2012) 258–268.
  • 16. Martini, S., D'Addario, C., Colacevich, A., Focardi, S., Borghini, F., Santucci, A., Figura, N. and Rossi, C. Antimicrobial activity against Helicobacter pylori strains and antioxidant properties of blackberry leaves (Rubus ulmifolius) and isolated compounds. Int. J. Antimicrob. Agents 34 (2009) 50–59.
  • 17. Bordonaba J.G. and Terry L.A. Biochemical profiling and chemometric analysis of seventeen UK-grown black currant cultivars. J. Agric. Food Chem. 56 (2008) 7422–7430.
  • 18. Swain T and Hillis W.E. The phenolics constituents of Prunus domestica II. J. Sci. Food Agric. 10 (1959) 63–68.
  • 19. Rosch, D., Bergmann, M., Knorr, D. and Kroh, L.W. Structure-antioxidant efficiency relationships of phenolic compounds and their contribution to the antioxidant activity of sea buckthorn juice. J. Agric. Food Chem. 51 (2003) 4233–4239.
  • 20. Hartzfeld, P.W., Forkner, R., Hunter, M.D. and Hagerman, A.E. Determination of hydrolyzable tannins (gallotannins and ellagitannins) after reaction with potassium iodate. J. Agric. Food Chem. 50 (2002) 1785– 1790.
  • 21. Dyrby, M., Westergaard, N. and Stapelfeldt, H. Light and heat sensitivity of red cabbage extract in soft drink model systems. Food Chem. 72 (2001) 431–437.
  • 22. Awah, F.M., Uzoegwu, P.N., Ifeonu, P., Oyugi, J.O., Rutherford, J., Yao, X., Fehrmann, F., Fowke, K.R. and Eze, M.O. Free radical scavenging activity, phenolic contents and cytotoxicity of selected Nigerian medicinal plants. Food Chem. 131 (2012) 1279–1286.
  • 23. Nardini, M., Cirillo, M., Natella, F., Mencarelli, D., Comisso, A. and Scaccini, C. Detection of bound phenolic acids: Prevention by ascorbic acid and ethylenediaminetetraacetic acid of degradation of phenolic acid during alkaline hydrolysis. Food Chem. 79 (2002) 119–124.
  • 24. Zadernowski, R., Naczk, M. and Nesterowicz, J. Phenolic acid profiles in some small berries. J. Agric. Food Chem. 53 (2005) 2118–2124.
  • 25. Benzie, I.F. and Strain, J.J. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal. Biochem. 239 (1996) 70–76.
  • 26. Kim, D.O., Lee, K.W., Lee, H.J. and Lee, C.Y. Vitamin C equivalent antioxidant capacity (VCEAC) of phenolic phytochemicals. J. Agric. Food Chem. 50 (2002) 3713–3717.
  • 27. Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M. and RiceEvans, C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med. 26 (1999) 1231–1237.
  • 28. Mosmann, T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 65 (1983) 55–63.
  • 29. Geladopoulos T.P., Sotiroudis T.G. and Evangelopoulos A.E. A malachite green colorimetric assay for protein phosphatase activity. Anal. Biochem. 192 (1991) 112–116.
  • 30. Smith, P.K., Krohn, R.I., Hermanson, G.T., Mallia, A.K., Gartner, F.H., Provenzano, M.D., Fujimoto, E.K., Goeke, N.M., Olson, B.J. and Klenk, D.C. Measurement of protein using bicinchoninic acid. Anal. Biochem. 150 (1985) 76–85.
  • 31. Brown, C.A., Bolton-Smith, C., Woodward, M. and Tunstall-Pedoe, H. Coffee and tea consumption and the prevalence of coronary heart disease in men and women: results from the Scottish Heart Health Study. J. Epidemiol. Community Health 47 (1993) 171–175.
  • 32. Yochum, L., Kushi, L.H., Meyer, K. and Folsom, A.R. Dietary flavonoid intake and risk of cardiovascular disease in postmenopausal women. Am. J. Epidemiol. 149 (1999) 943–949.
  • 33. Castaner, O., Covas, M.I., Khymenets, O., Nyyssonen, K., Konstantinidou, V., Zunft, H.F., de la Torre, R., Munoz-Aguayo, D., Vila, J. and Fito, M. Protection of LDL from oxidation by olive oil polyphenols is associated with a downregulation of CD40-ligand expression and its downstream products in vivo in humans. Am. J. Clin. Nutr. 95 (2012) 1238–1244.
  • 34. Fuentes, E., Fuentes, F. and Palomo, I. Mechanism of the anti-platelet effect of natural bioactive compounds: Role of peroxisome proliferator-activated receptors activation. Platelets (2013) Epub ahead of print. DOI:10.3109/09537104.2013.849334.
  • 35. Abe, R., Beckett, J., Abe, R., Nixon, A., Rochier, A., Yamashita, N. and Sumpio, B. Olive oil polyphenols differentially inhibit smooth muscle cell proliferation through a G1/S cell cycle block regulated by ERK1/2. Int. J. Angiol. 21 (2012) 69–76.
  • 36. Oszmianski, J., Wojdylo, A., Gorzelany, J. and Kapusta, I. Identification and characterization of low molecular weight polyphenols in berry leaf extracts by HPLC-DAD and LC-ESI/MS. J. Agric. Food Chem. 59 (2011) 12830–12835.
  • 37. Venskutonis, P.R., Dvaranauskaite, A. and Labokas, J. Radical scavenging activity and composition of raspberry (Rubus idaeus) leaves from different locations in Lithuania. Fitoterapia 78 (2007) 162–165.
  • 38. Gudej, J. Kaempferol and quercetin glycosides from Rubus idaeus L. leaves. Acta Pol. Pharm. 60 (2003) 313–315.
  • 39. Gudej, J. and Tomczyk, M. Determination of flavonoids, tannins and ellagic acid in leaves from Rubus L. species. Arch. Pharm. Res. 27 (2004) 1114–1119.
  • 40. Serteser, A., Kargioglu, M., Gok, V., Bagci, Y., Ozcan, M.M. and Arslan, D. Determination of antioxidant effects of some plant species wild growing in Turkey. Int. J. Food. Sci. Nutr. 59 (2008) 643–651.
  • 41. Kaneider, N.C., Mosheimer, B., Reinisch, N., Patsch, J.R. and Wiedermann, C.J. Inhibition of thrombin-induced signaling by resveratrol and quercetin: effects on adenosine nucleotide metabolism in endothelial cells and plateletneutrophil interactions. Thromb. Res. 114 (2004) 185–194.
  • 42. Schmatz, R., Mann, T.R., Spanevello, R., Machado, M.M., Zanini, D., Pimentel, V.C., Stefanello, N., Martins, C.C., Cardoso, A.M., Bagatini, M., Gutierres, J., Leal, C.A., Pereira, L.B., Mazzanti, C., Schetinger, M.R. and Morsch, V.M. Moderate red wine and grape juice consumption modulates the hydrolysis of the adenine nucleotides and decreases platelet aggregation in streptozotocin-induced diabetic rats. Cell Biochem. Biophys. 65 (2013) 129–143.
  • 43. Ashraf, M., Shah, S.M.A.S, Ahmad, I., Ahmad, S., Arshad, S., Ahmad, K. and Nasim, F.H. Nucleoside triphosphate diphosphohydrolases (NTPDase) inhibitory activity of some medicinal plants. J. Med. Plants Res. 5 (2011) 2090–2094.
  • 44. Lecka, J., Rana, M.S. and Sevigny, J. Inhibition of vascular ectonucleotidase activities by the pro-drugs ticlopidine and clopidogrel favours platelet aggregation. Br. J. Pharmacol. 161 (2010) 1150–1160.
  • 45. Robson, S.C., Kaczmarek, E., Siegel, J.B., Candinas, D., Koziak, K., Millan, M., Hancock, W.W. and Bach, F.H. Loss of ATP diphosphohydrolase activity with endothelial cell activation. J. Exp. Med. 185 (1997) 153–163.
  • 46. Garcia-Hernandez, M.H., Portales-Cervantes, L., Cortez-Espinosa, N., Vargas-Morales, J.M., Fritche Salazar, J.F., Rivera-Lopez, E., RodriguezRivera, J.G., Quezada-Calvillo, R. and Portales-Perez, D.P. Expression and function of P2X(7) receptor and CD39/Entpd1 in patients with type 2 diabetes and their association with biochemical parameters. Cell Immunol. 269 (2011) 135–143.
  • 47. Leeuwenberg, J.F., Smeets, E.F., Neefjes, J.J., Shaffer, M.A., Cinek, T., Jeunhomme, T.M., Ahern, T.J. and Buurman, W.A. E-selectin and intercellular adhesion molecule-1 are released by activated human endothelial cells in vitro. Immunology 77 (1992) 543–549.
  • 48. Grigore, A., Colceru-Mihul, S., Litescu, S., Panteli, M. and Rasit, I. Correlation between polyphenol content and anti-inflammatory activity of Verbascum phlomoides (mullein). Pharm. Biol. 51 (2013) 925–929.
  • 49. Mochizuki, M., Kajiya, K., Terao, J., Kaji, K., Kumazawa, S., Nakayama, T. and Shimoi, K. Effect of quercetin conjugates on vascular permeability and expression of adhesion molecules. Biofactors 22 (2004) 201–204.
  • 50. Kolodziej, H. and Kiderlen, A.F. Antileishmanial activity and immune modulatory effects of tannins and related compounds on Leishmania parasitised RAW 264.7 cells. Phytochemistry 66 (2005) 2056–2071.
  • 51. Durgo, K., Belscak-Cvitanovic, A., Stancic, A., Franekic, J. and Komes, D. The bioactive potential of red raspberry (Rubus idaeus L.) leaves in exhibiting cytotoxic and cytoprotective activity on human laryngeal carcinoma and colon adenocarcinoma. J. Med. Food. 15 (2012) 258–268.
  • 52. Hu, J., Zhao, J., Chen, W., Lin, S., Zhang, J. and Hong, Z. Hepatoprotection of 1beta-hydroxyeuscaphic acid - the major constituent from Rubus aleaefolius against CCl4-induced injury in hepatocytes cells. Pharm. Biol. 51 (2013) 686–690.
  • 53. Zhao, J., Chen, X., Lin, W., Wu, G., Zhuang, Q., Zhong, X., Hong, Z. and Peng, J. Total alkaloids of Rubus aleaefolius Poir inhibit hepatocellular carcinoma growth in vivo and in vitro via activation of mitochondrialdependent apoptosis. Int. J. Oncol. 42 (2013) 971–978.
  • 54. Zheng, Z.X., Zhang, L.J., Huang, C.X., Huang, Q.L., Wei, X.D., Wu, X.Y. and Zhou, W.M. [Antitumour effect of total saponins of Rubus parvifolius on malignant melanoma]. Zhongguo Zhong. Yao Za Zhi. 32 (2007) 2055– 2058.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-5859c9e6-d933-4bd8-81c2-cea314a29dcb
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.