Ex vitro and in vitro Paphiopedilum delenatii Guillaumin stem elongation under light-emitting diodes and shoot regeneration via stem node culture

• Autorzy: Luan V.Q. , Huy N.P. , Nam N.B. , Huong T.T. , Hien V.T. , Hien N.T.T. , Hai N.T. , Thinh D.K. , Nhut D.T.
• Języki publikacji: EN

Abstrakty

EN

Paphiopedilum spp. are high value horticultural crops; however, success in Paphiopedilum spp. culture is still limited. It is therefore necessary to find out adequate solutions to improve the culture techniques of these recalcitrant species. In the present study, ex vitro shoots exposed to LEDs for shoot elongation and in vitro multiplication of P. delenatii from single nodes were investigated. One-month-old ex vitro shoots with 1.5–2.0 cm length were grown under various light conditions including monochromatic blue and red LEDs, mixtures of blue and red LEDs, and the darkness for investigations of the shoot elongation. The results indicated that the best stem elongation was obtained under monochromatic blue LED (100B) after 4 months of culture. The highest shoot regeneration rate (45 %) was also recorded in shoots cultured on liquid SH medium containing 30 g l-1 sucrose, 1.0 mg l-1 TDZ and 0.3 mg l-1 NAA with cotton wool plug as the substrate and exposed to 100B after 45 days. P. delenatii young shoots, which had formed 5 leaves, were placed into SH medium supplemented with 0.5 mg l-1 - BA, 0.5 mg l-1 NAA, 30 g l-1 sucrose, and 1 g l-1 activated charcoal in the darkness for stem node elongation before transferred into greenhouse. After 120 days, the average shoot length was 10.5 cm, and five new leaves and five single nodes per shoot were observed. These shoots then were maintained under fluorescent light for 60 days. Although the shoots regenerated new leaves, no new node was observed. After 180 days of culture, each shoot was cut into five single nodes, and cultured on fern fibers. These shoots showed to grow vigorously after 12 months.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2015
• Tom: 37
• Numer: 07
• Opis fizyczny: fig.,ref.

Twórcy

autor

Luan V.Q.

• Tay Nguyen Institute for Scientific Research, Vietnam Academy of Science and Technology, Dalat, Vietnam

autor

Huy N.P.

• Tay Nguyen Institute for Scientific Research, Vietnam Academy of Science and Technology, Dalat, Vietnam

autor

Nam N.B.

• Tay Nguyen Institute for Scientific Research, Vietnam Academy of Science and Technology, Dalat, Vietnam

autor

Huong T.T.

• Tay Nguyen Institute for Scientific Research, Vietnam Academy of Science and Technology, Dalat, Vietnam

autor

Hien V.T.

• Tay Nguyen Institute for Scientific Research, Vietnam Academy of Science and Technology, Dalat, Vietnam

autor

Hien N.T.T.

• Tay Nguyen Institute for Scientific Research, Vietnam Academy of Science and Technology, Dalat, Vietnam

autor

Hai N.T.

• Tay Nguyen Institute for Scientific Research, Vietnam Academy of Science and Technology, Dalat, Vietnam

autor

Thinh D.K.

• Institute of Agricultural Science for Southern Vietnam, Ho Chi Minh, Vietnam

autor

Nhut D.T.

• Tay Nguyen Institute for Scientific Research, Vietnam Academy of Science and Technology, Dalat, Vietnam

Bibliografia

• Arditti J, Ernst R (1992) Micropropagation of orchids. John Wiley Sons Inc, Hoboken, pp 434–466
• Arditti J, Ernst R (1993) Several early scientific investigators and their research: A contribution towards the history of orchids in the United States of America. In: Rittershausen W (ed) 100 years of orchids, the Orchid review centenary year book 1893–1993.
• Bradfield Books, Creat Bardfliled, Braintree, Essex CM7 4RZ, UK, pp 125–142
• Arditti J, Mosich SK, Ball EA (1973) Dendrobium node cultures: a new means of clonal propagation. Aust Orchid Rev 38:175–179
• Averyanov L, Cribb P, Loc PK, Hiep NT (2003) Slipper orchids of Vietnam. Compass Press Limited, Kew
• Barker AV, Arker K, Corey A (1987) Ammonium-induced ethylene evolution by horticultural crops. Hort Sci 22:381
• Behringer FJ, Davies PJ (1992) Indole-3-acetic acid levels after phytochrome-mediated changes in the stem elongation rate of dark- and light-grown Pisum seedlings. Planta 188:85–92
• Bressan PH, Kim YJ, Hyndman SE, Hasegawa PM, Bressan RA (1982) Factors affecting in vitro propagation of rose. J Am Soc Hortic Sci 107:979–990
• Brown CS, Schuerger AC, Sager JC (1995) Growth and photomorphogenesis of pepper plants under red light-emitting diodes with supplemental blue or far-red lighting. J Amer Soc Horti Sci 120:808–813
• Bubeck SK (1973) A study of Paphiopedilum meristem culture. Ph.D Thesis, Rutgers University, University Microfilm International, Ann Arbor, Michigan
• Bula RJ, Morrow RC, Tibbitts TW, Ignatius RW, Martin TS, Barta DJ (1991) Light emitting diodes as a radiation source for plants. HortScience 26:203–205
• Chen Y, Pileuk C (1995) Effects of thidiazuron and N6-benzylaminopurine on shoot regeneration of Phalaenopsis. Plant Growth Regul 16:99–101
• Chen TY, Chen JT, Chang WC (2004) Plant regeneration through direct shoot bud formation from leaf cultures of Paphiopedilum orchids. Plant Cell Tiss Org Cult 76:11–15
• Chyuam YN, Saleh NM (2011) In vitro propagation of Paphiopedilum orchid through formation of protocorm-like bodies. Plant Cell Tiss Org Cult 105:193–202
• Chyuam YN, Saleh NM, Zaman FQ (2010) In vitro multiplication of the rare and endangered slipper orchid, Paphiopedilum rothschildianum (Orchidaceae). Afr J Biotech 9(14):2062–2068
• Cohen L, Gepstein S, Horwitz BA (1991) Similarity between cytokinin and blue light inhibition of Cucumber hypocotyl elongation. Plant Physiol 95(1):77–81
• Dewir YH, Chakrabarty D, Hahn EJ, Paek KY (2007) Flowering of Euphorbia millii plantlets in vitro as affected by paclobutrazol, light emitting diodes (LEDs) and sucrose. Acta Hort 764:169–173
• Duncan DB (1995) Multiple range and multiple F test. Biometrics 11:1–42
• Duong TN, Hong LTA, Watanabe H, Goi M, Tanaka M (2003) Efficiency of a novel culture system by using light-emitting diode (LED) on in vitro and subsequent growth of micropropagated banana plantlets. Acta Hort 616:121–127
• Felker FC, Doehlert DC, Eskins K (1995) Effects of red and blue light on the composition and morphology of maize kernels grown in vitro. Plant Cell Tiss Org Cult 42:147–152
• Fukuda N, Fujitan M, Ohta Y, Sase S, Nishimura S, Ezura H (2008) Directional blue light irradiation triggers epidermal cell elongation of abaxial side resulting in inhibition of leaf epinasty in geranium under red light condition. Sci Hort 115:176–182
• Gamborg O, Miller R, Ojima K (1968) Nutrient requirement suspensions cultures of soybean root cells. Exp Cell Res 50(1):151–158
• Giedre S, Ausra B, Akvile U, Gintare S, Pavelas D (2010) The effect of red and blue light component on the growth and development of frigo strawberries. Zemdirbyste Agr 97(2):99–104
• Giliberto L, Perrotta G, Pallara P, Weller JL, Fraser PD, Bramley PM, Fiore A, Tavazza M, Giuliano G (2005) Manipulation of the blue light photoreceptor cryptochrome 2 in tomato affects vegetative development, flowering time, and fruit antioxidant content. Plant Physiol 137:199–208
• Hahn EJ, Bae CH, Lee YB (1998) Growth and leaf-surface characteristics of Chrysanthemum plantlets between micropropagation and microponic system. J Kor Soc Hort Sci 39:838–842
• Hahn EJ, Kozai T, Paek KY (2000) Blue and red light-emitting diodes with or without sucrose and ventilation affects in vitro growth of Rehmannia glutinosa plantlets. Plant Biol 43:247–250
• Hirai T, Amaki W, Watanabe H (2006) Action of blue or red monochromatic light on stem intermodal growth depends on plant species. Acta Hort 711:345–349
• Hong PI, Chen JT, Chang WC (2008) Plant regeneration via protocorm-like body formation and shoot multiplication from seed derived callus of a maudiae type slipper orchid. Acta Physiol Plant 30:755–759
• Huang LC (1988) A procedure for asexual multiplication of Paphiopedilum in vitro. Am Orchid Soc Bul 57:274–278
• Huang LC, Lin CJ, Kou CI, Huang BL, Murashige T (2001) Paphiopedilum cloning in vitro. Sci Hort 91:111–121
• Jao RC, Fang W (2004) Effects of frequency and duty ratio on the growth of potato plantlets in vitro using light-emitting diodes. HortScience 39:375–379
• Jao RC, Lai CC, Fang W, Chang SF (2005) Effects of red light on the growth of Zantedeschia plantlets in vitro and tuber formation using light-emitting diodes. HortScience 40:436–438
• Kim SJ, Hahn EJ, Heo JW, Paek KY (2004) Effects of LEDs on net photosynthetic rate, growth and leaf stomata of Chrysanthemum plantlets in vitro. Sci Hort 101:143–151
• Koch L (1974) Erbgleiche Vermehrung von Phalaenopsis in vitro. Gartenwelt 74:482–484
• Kurilčik A, Miklušyte-Čanova R, Dapkünien_e S, Žilinskait _e S, Kurilčik G, Tamulaitis G, Duchovskis P, Žukauskas A (2008) In vitro culture of Chrysanthemum plantlets using light-emitting diodes. Cent Eur J Biol 3:161–167
• Le BV, Hong Phuong NT, Anh Hong LT, Van Tran Thanh K (1996) High frequency shoot regeneration from Rhynchostylis gigantea (Orchidaceae) using thin cell layer. Plant Growth Regul 28:179–185
• Lee AE, Tewari RK, Hahn EJ, Paek KY (2007) Photon flux density and light quality induce changes in growth, stomatal development, photosynthesis and transpiration of Withania somnifera (L.) Dunal. plantlets. Plant Cell Tiss Org Cult 90:141–151
• Li Q, Kubota C (2009) Effects of supplemental light quality on growth and phytochemicals of baby leaf lettuce. J Environ Exp Bot 67:59–64
• Lian ML, Murthy HN, Paek KY (2002) Effect of light emitting diodes (LEDs) on the in vitro induction and growth of bulblets of Lilium oriental hybrid’Pesaro’. Sci Hort 94:365–370
• Liao YJ, Tsai YC, Sun YW, Lin RS, Wu FS (2011) In vitro shoot induction and plant regeneration from flower buds in Paphiopedilum orchids. In Vitro Cell Dev Biol Plant 47:702–709
• Lin YH, Chang C, Chang WC (2000) Plant regeneration from callus culture of a Paphiopedilum hybrid. Plant Cell Tiss Org Cult 62:21–25
• Luan VQ, Huy NP, Chien HX, Huong TT, Hien VT, Nam NB, Thinh DK, Nhut DT (2012) Micropopagation of Paphiopedilum callosum. J Biotech 10(3):487–494
• Marin JA, Jones OP, Hadlow WCC (1993) Micropropagation of columnar apple trees. J Hortic Sci 68:289–297
• Miyashita Y, Kitaya Y, Kozai T, Kimura T (1995) Effects of red and far-red light on the growth and morphology of potato plantlets in vitro: using light emitting diode as a light source for micropropagation. Acta Hort 393:189–194
• Morini S, Trinci M, Zacchini M (1991) Effect of different photoperiods on in vitro growth of plum rootstock. Plant Cell Tiss Org Cult 25:141–145
• Mosich SK, Ball EA, Arditti J (1973) Propagation clonal de Dendrobium por medio del cultivo de nodos. Orquidea (Mexico) 3:244–260
• Mosich SK, Ball EA, Arditti J (1974a) Clonal propagation of orchids by means of node cultures. Am Orchid Soc Bull 43:1005–1061
• Mosich SK, Ball EA, Flick BH, Arditti J (1974b) Klonvermehrung von Dendrobium durch die Kultivierung von Stammnodien. Orchidee 25:129–134
• Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue. Physiol Plant 15:473–496
• Nanya K, Ishigami Y, Hikosaka S, Goto E (2012) Effects of blue and red light on stem elongation and flowering of tomato seedlings. Acta Hort 956:261–266
• Ng CY, Saleh NM (2011) In vitro propagation of Paphiopedilum orchid through formation of protocorm-like bodies. Plant Cell Tiss Org Cult 105:193–202
• Nhut DT, Takamura T, Watanabe H, Murakami A, Murakami K, Tanaka M (2002) Sugar-free micropropagation of Eucalyptus citriodora using light-emitting diode (LEDs) and film-rockwool culture system. Environ Control Biol 40:147–155
• Nhut DT, Takamura T, Watanabe H, Okamoto K, Tanaka M (2003) Responses of strawberry plantlets cultured in vitro under superbright red and blue light-emitting diodes (LEDs). Plant Cell Tiss Org Cult 73:43–52
• Nhut DT, Takamura T, Watanabe H, Okamoto K, Tanaka M (2005a) Artificial light source using light-emitting diodes (LEDs) in the efficient micropropagation of Spathiphyllum plantlets. Acta Hort 692:137–142
• Nhut DT, Trang PTT, Vu NH, Thuy DTT, Khiem DV, Van Tran Thanh K (2005b) A wounding method and liquid culture in Paphiopedilum delenatii propagation. Prop Ornam Plant 5(3):156–161
• Nhut DT, Thuy DTT, Don NT, Luan VQ, Hai NT, Van Tran Thanh K, Chinnappa CC (2007) Stem elongation of Paphiopedilum delenatii Guillaumin and shoot regeneration via stem node culture. Prop Ornam Plant 7(1):29–36
• Noiton D, Vine JH, Mullins MG (1992) Effects of serial subculture in vitro on the endogenous levels of indole-3-acetic acid and abscisic acid and rootability in micro-cuttings of ‘Jonathan’ apple. Plant Growth Regul 11:377–383
• Nuraini I, Shaib MJ (1992) Micropropagation of orchids using scape nodes as the explant material. Acta Hortic 292:169–172
• Parker MW, Hendricks SB, Borthwick HA, Wxent FW (1949) Spectral sensitivity for leaf and stem growth of etiolated pea seedlings and their similarity to action spectra for photoperiodism. Amer J Bot 36:194–204
• Parks BM, Folta KM, Spalding EP (2001) Photocontrol of stem growth. Curr Opin Plant Biol 4:436–440
• Patcharawadee W, Eric B, Kongkanda C, Sureeya T (2011) Effect of cytokinins (BAP and TDZ) and auxin (2,4-D) on growth and development of Paphiopedilum callosum. Kasetsart J Nat Sci 45:12–19
• Platten JD, Foo E, Elliott RC, Hecht V, Reid JB, Weller JL (2005) Cryptochrome 1 contributes to blue-light sensing in pea. Plant Physiol 139:1472–1482
• Poudel PR, Kataoko I, Mochioka R (2008) Effect of red-and bluelight-emitting diodes on growth and morphogenesis of grapes. Plant Cell Tiss Org Cult 92:147–153
• Rajapakse NC, Pollock RK, McMahon MJ (1992) Interpretation of light quality measurements and plant response in spectral filter research. J Hort Sci 27:1208–1211
• Ramage CM, Williams RR (2001) Mineral nutrition and plant morphogenesis. In Vitro Cell Dev Biol-Plant 38:116–124
• Rotor G (1949) A method of vegetative propagation of Phalaenopsis species and hybrids. Am Orchid Soc Bull 18:738–739
• Sandal I, Bhattacharya A, Ahuja PS (2001) An efficient liquid culture system for tea shoot proliferation. Plant Cell Tiss Org Cult 65:75–80
• Schaefer S, Medeiro SA, Ramírez JA, Galagovsky LR, Pereira-Netto AB (2002) Brassinosteroid-driven enhancement of the in vitro multiplication rate for the marubakaido apple rootstock [Malus prunifolia (Willd.) Borkh]. Plant Cell Rep 20:1093–1097
• Schenk RU, Hildebrandt AC (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyle-donous plant cell cultures. Can J Bot 50:199–204
• Sim GE, Loh CS, Goh CJ (2007) High frequency early in vitro flowering of Dendrobium Madame Thong-In (Orchidaceae). Plant Cell Rep 26:383–393
• Smith MAL, Spomer LA (1994) Vessels, gels, liquid media and support systems. In: Aiktken-Christie J, Kosai T, Smith MAL (eds) Automation and environmental control in plant tissue culture. Kluwer Academic Publishers, Dordrecht, pp 371–404
• Sorce C, Picciarelli P, Calistri G, Lercari B, Ceccarelli N (2008) The involvement of indole-3-acetic acid in the control of stem elongation in dark- and light-grown pea (Pisum sativum) seedlings. J Plant Physiol 165(5):482–489
• Stewart J, Button J (1975) Tissue culture studies in Paphiopedilum. Amer Orchid Soc Bull 35:88–95
• Tanaka M, Takamura T, Watanabe H, Endo M, Yanagi T, Okamoto K (1998) In vitro growth of Cymbidium plantlets cultured under super bright and blue light-emitting diodes (LEDs). J Hort Sci Biotech 73:39–44
• Teob ES (1989) Orchids of Asia. Times Books International, Singapore, p 317
• Tibbitts TW, Morgan DC, Warrington JJ (1983) Growth of lettuce, spinach, mustard and wheat plants under four combinations of high-pressure sodium, metal halide and tungsten halogen lamps at equal PPFD. J Amer Hort Sci 108:622–630
• Tomoki H, Tadashi M, Kazuhiro Y, Yuko Y, Keimei O (2014) Synthesis of new brassinosteroid biosynthesis inhibitor with coumarin moiety as a fluorescent probe. Inter J Chem Eng App 5(5):379–383
• Udomdee W, Wen PJ, Chin SW, Chen FC (2012) Shoot multiplication of Paphiopedilum orchid through in vitro cutting methods. Afr J Biotech 11(76):14077–14082
• Urata U, Iwanaga ET (1965) The use of Ito-type vials for vegetative propagation of Phalaenopsis. Am Orchid Soc Bull 35:410–413
• Vacin E, Went F (1949) Some pH changes in nutrient solution. Bot Gar Conser New 110:605–613
• Wang Q, Tang H, Quan Y, Zhou G (1994) Phenol induced browning and establishment of shoot tip explants of ‘Fuji’ apple and ‘Jinhua’ pear cultured in vitro. J Hortic Sci 69:833–839
• Wongnok A, Piluek C, Techasilpitak T, Tantivivat S (2008) Effects of light emitting diodes on micropropagation of Phalaenopsis orchids. Acta Hort 788:149–156
• Yepes LM, Aldwinckle HS (1994) Micropropagation of thirteen Malus cultivars and rootstocks, and effect of antibiotics on proliferation. Plant Growth Regul 15:55–67
• Ziv M (1989) Enhanced shoot and cormlet proliferation in liquid cultured gladiolus buds by growth retardants. Plant Cell Tiss Org Cult 17:101–110
• Ziv M, Halevy AH (1983) Control of oxidative browning and in vitro propagation of Strelitzia reginae. HortScience 18:1085–1087

Identyfikatory

DOI

10.1007/s11738-015-1886-8