Quantitative analysis of oestrogen receptor ? and Beta gene expression during prolonged culture of pig granulosa cells

• Autorzy: Chronowska E. , Kott T.
• Języki publikacji: EN

Abstrakty

EN

The aim of the present work was to determine the relative transcript abundance of the oestrogen receptor alpha (ERα) and beta (ERβ) gene in long-term cultures (up to 18 days) of pig granulosa cells (GC) obtained from small (1-2 mm; SF-GC) and large (5-7 mm; LF-GC) follicles. GC were cultured in the presence of 10% foetal calf serum (FCS) and one of the following growth factors: leukaemia inhibitory factor (LIF), basic fibroblast growth factor (bFGF), or stem cell factor (SCF). The relative transcript abundance of the investigated genes was measured by real time PCR (RTPCR). In comparison with the control (medium only), LIF significantly increased (P<0.05-0.01) ERα gene expression in SF-GC after 3, 15 and 18 days of culture and after 12 days in LF-GC. The ERβ mRNA level in SF-GC was significantly decreased (P<0.01) by all of the investigated factors after 3, 6, 9 and 12 days of incubation. In conclusion, expression of both types of oestrogen receptors during prolonged culture of pig GC under different conditions suggests the role of oestrogens acting via their receptors in maintaining survivability of porcine granulosa cells in vitro.

• Wydawca: -
• Czasopismo: Journal of Animal and Feed Sciences
• Rocznik: 2012
• Tom: 21
• Numer: 2
• Opis fizyczny: p.313-123,fig.,ref.

Twórcy

autor

Chronowska E.

• Department of Biology of Reproduction, Institute of Animal Science, Pratelství 815, 104 00 Prague-Uhríneves, Czech Republic

autor

Kott T.

• Department of Molecular Genetics, Institute of Animal Science, Pratelství 815, 104 00 Prague-Uhríneves, Czech Republic

Bibliografia

• Bauer S., Patterson P.H., 2006. Leukemia inhibitory factor promotes neural stem cell self-renewal in the adult brain. J. Neurosci. 26, 12089-12099
• Brankin V., Mitchell M.R., Webb B., Hunter M.G., 2003. Paracrine effects of oocyte secreted factors and stem cell factor on porcine granulosa and theca cells in vitro. Reprod. Biol. Endocrinol. 12, 1-55
• Chronowska E., Kott T., 2011. Optimization of conditions of prolonged culture of pig granulosa cells in vitro. Ann. Anim. Sci. 11, 383-392
• Chronowska E., Tomanek M., Kott T., 2009. Effect of aromatase inhibitor (fadrozole) on proliferation, estradiol production and telomerase activity in pig granulosa cells in vitro. Czech J. Anim. Sci. 54, 566-574
• Chronowska E., Tomanek M., Kott T., 2010. Effect of antioestrogens on proliferation, differentiation and telomerase activity of pig granulosa cells in vitro. J. Anim. Feed Sci. 19, 577-589
• Daheron L., Opitz S.L., Zaehres H., Lensch M.W., Andrews P.W., Itskovitz-Eldor J., Daley G.Q., 2004. LIF/STAT3 signaling fails to maintain self-renewal of human embryonic stem cells. Stem Cells 22, 770-778
• Dhingra K., Sahin A., Emami K., Hortobagyi G.N., Estrov Z., 1998. Expression of leukemia inhibitory factor and its receptor in breast cancer: a potential autocrine and paracrine growth regulatory mechanism. Breast Cancer Res. Treat. 48, 165-174
• Diaz F.J., Wiltbank M.C., 2004. Acquisition of luteolytic capacity: changes in prostaglandin F2a regulation of steroid hormone receptors and estradiol biosynthesis in pig corpora lutea. Biol. Reprod. 70, 1333-1339
• Estrov Z., Samal B., Lapushin R., Kellokumpu-Lehtinen P., Sahin A.A., Kurzrock R., Talpaz M., Aggarwal B.B., 1995. Leukemia inhibitory factor binds to human breast cancer cells and stimulates their proliferation. J. Interferon Cytokine Res. 15, 905-913
• Garor R., Abir R., Erman A., Felz C., Nitke S., Fisch B., 2009. Effects of basic fibroblast growth factor on in vitro development of human ovarian primordial follicles. Fertil. Steril. 91, Suppl. 5, 1967-1975
• Hewitt S.C., Harrell J.C., Korach K.S., 2005. Lesson in estrogen biology from knockout and transgenic animals. Annu. Rev. Physiol. 67, 285-308
• Jiang Y, Vaessen B., Lenvik T., Blackstad M., Reyes M., Verfaillie C.M., 2002. Multipotent progenitor cells can be isolated from postnatal murine bone marrow, muscle and brain. Exp. Hematol. 30, 896-904
• Kalabis J., Patterson M.J., Enders G.H., Marian B., Iozzo R.V., Rogler G., Gimotty P.A., Herlyn M., 2003. Stimulation of human colonic epithelial cells by leukemia inhibitory factor is dependent on collagen-embedded fibroblast in organotypic culture. FASEB J. 17, 1115-1117
• Kent D., Copley M., Benz C., Dykstra B., Bowie M., Eaves C., 2008. Regulation of hematopoietic stem cells by the steel factor/KIT signaling pathway. Clin. Cancer Res. 14, 1926-1930
• Knapczyk K., Duda M., Durlej M., Galas J., Koziorowski M., Slomczynska M., 2008. Expression of estrogen receptor alpha (ERalpha) and estrogen receptor beta (ERbeta) in the ovarian follicles and corpora lutea of pregnant swine. Domest. Anim. Endocrinol. 35, 170-179
• Ko K., Arauzo-Bravo M.J., Kim J., Stehling M., Schoeler H.R., 2010. Conversion of adult mouse unipotent germline stem cells into pluripotent stem cells. Nature Protocols 5, 921-928
• Kossowska-Tomaszczuk K., De Geyter C., De Geyter M., Martin I., Holzgreve W., Scherberich A., Zhang H., 2009. The multipotency of luteinizing granulosa cells collected from mature ovarian follicles. Stem Cells 27, 210-219
• Lysdahl H., Gabrielsen A., Minger S.L., Patel M.J., Fink T., Petersen K., Ebbesen P., Zachar V., 2006. Derivation and characterization of four new human embryonic stem cell lines: the Danish experience. Reprod. Biomed. Online 12, 119-126
• Niswender G.D., Juengel J.L., Silva P.J., Rollyson M.K., McIntush E.W., 2000. Mechanisms controlling the function and the life span of the corpus luteum. Physiol. Rev. 80, 1-24
• Picton H.M., Campbell B.K., Hunter M.G., 1999. Maintenance of oestradiol production and expression of cytochrome P450 aromatase enzyme mRNA in long-term serum- free cultures of pig granulosa cells. J. Reprod. Fertil. 115, 67-77
• Rossi P., Sette C., Dolci S., Geremia R., 2000. Role of c-kit in mammalian spermatogenesis. J. Endocrinol. Invest. 23, 609-615
• Sharma G.T., Dubey P.K., Kumar G.S., 2010. Effects of IGF-1, TGF-alpha plus TGF-beta1 and bFGF on in vitro survival, growth and apoptosis in FSH-stimulated buffalo (Bubalis bubalus) preantral follicles. Growth Horm. IGF Res. 20, 319-325
• Slomczynska M., Duda M., Galas J., 2001. Estrogen receptor alpha and beta expression in the porcine ovary. Folia Histochem. Cytobiol. 39, 137-138
• Słomczyńska M., Wozniak J., 2001. Differential distribution of estrogen receptor-b and estrogen receptor-a in the porcine ovary. Exp. Clin. Endocrinol. Diabetes 109, 238-244
• Yamagata Y., Nakamura Y., Umayahara K., Harad A., Takayama H., Sugino N., Kato H., 2002. Changes in telomerase activity in experimentally induced atretic follicles of immature rats. Endocrine J. 49, 589-595