Effects of macro elements and nitrogen source on biomass accumulation and bacoside A production from adventitious shoot cultures of Bacopa monnieri (L.)

• Autorzy: Naik P.M. , Manohar S. H. , Murthy H. N.
• Języki publikacji: EN

Abstrakty

EN

The present work deals with optimization of adventitious shoot culture of Bacopa monnieri for the production of biomass and bacoside A and has investigated the effects of macro elements (NH₄NO₃, KNO₃, CaCl₂, MgSO₄ and KH₂PO₄) and nitrogen source [NH₄⁺/NO₃⁻] of Murashige and Skoog (Physiol Plant 15:473–497, 1962) medium (MS) on accumulation of biomass and bacoside A content. Optimum number of adventitious shoots (99.33 shoots explant⁻¹), fresh weight (1.841 g) and dry weight (0.150 g) were obtained in the medium with 2.09 strength of NH₄NO₃. The highest production of bacoside A content was also recorded in the medium of 2.09 NH₄NO₃, which produced 17.935 mg g⁻¹ DW. The number of adventitious shoot biomass and bacoside A content were optimum when the NO₃⁻ concentration was higher than that of NH₄⁺. Maximum number of shoots (70.00 shoots explant⁻¹), biomass (fresh weight 1.137 g and dry weight 0.080 g) and also bacoside A content (27.106 mg g⁻¹ DW) were obtained at NH₄⁺/NO₃⁻ ratio of 14.38/37.60 mM. Overall, MS medium supplemented with 2.0 × NH₄NO₃ is recommended for most efficient bacoside A production.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2011
• Tom: 33
• Numer: 4
• Opis fizyczny: p.1553-1557,fig.,ref.

Twórcy

autor

Naik P.M.

• Department of Botany, Karnatak University, Dharwad, 580 003, India

autor

Manohar S. H.

• Department of Botany, Karnatak University, Dharwad, 580 003, India

autor

Murthy H. N.

• Department of Botany, Karnatak University, Dharwad, 580 003, India

Bibliografia

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