Vitamin E protected the mouse adrenal gland against immobilization stress

• Autorzy: Asır F. , Nergiz Y. , Pala A.
• Języki publikacji: EN

Abstrakty

EN

In this study, we aimed to investigate the effects of vitamin E on mouse adrenal glands in immobilization stress. Twenty-eight male, 10-week-old, BALB/C mice weighing 30-45 grams were divided into four groups. Mice were placed in a cage where no movement was allowed 6 hours/day for 7 days for immobilization stress. 10 ml/kg vitamin E was administered orogastrically 1 hour before immobilization stress in the vitamin E and stress+vitamin E group. At the end of the 7th day, all the animals were subjected to elevated-plus maze (anxiety) and forced swimming (depression) tests. Left adrenal glands were dissected for routine paraffin tissue embedding protocol. Adrenal sections were stained with hematoxylin-eosin and Azan. Malonaldehyde (MDA) levels were also measured in the adrenal tissues. Anxiety level (0.023), depression level (p=0.042) and MDA values (p=0.01) were significantly increased in the stress group. Histological sections of the stress group showed cortical atrophy, medullary hypertrophy, vascular dilation and hemorrhage. Azan staining revealed a thinned capsule and corticomedullary fibrosis in the stress group. Pathologies induced by immobilization stress were mostly reversed after vitamin E administration. The results suggested that vitamin E alleviates adverse effects of immobilization stress (oxidative, behavioral and histopathologic changes) in mice.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2022
• Tom: 25
• Numer: 3
• Opis fizyczny: p.447-454,fig.,ref.

Twórcy

autor

Asır F.

• Department of Histology and Embryology, School of Medicine, Dicle University, Diyarbakır, Turkey

autor

Nergiz Y.

• Department of Histology and Embryology, School of Medicine, Dicle University, Diyarbakır, Turkey

autor

Pala A.

• Department of Histology and Embryology, School of Medicine, Dicle University, Diyarbakır, Turkey

Identyfikatory

DOI

10.24425/pjvs.2022.142029