Comparison of phenylethanoids content in Rhodiola kirilowii and Rhodiola rosea roots applying a newly developed UPLC-MS/MS method

• Autorzy: Gryszczynska A. , Lowicki Z. , Opala B. , Krajewska-Patan A. , Buchwald W. , Czerny B. , Mielcarek S. , Mrozikiewicz P. M.

Warianty tytułu

PL

Porównanie zawartości fenyloetanoidów w korzeniach Rhodiola kirilowii i Rhodiola rosea za pomocą metody UPLC-MS/MS

• Języki publikacji: EN

Abstrakty

EN

A concentration of two phenylethanoids in the roots of two species: Rhodiola kirilowii and rosea were compared, aqueous and hydroalcoholic extracts from those plants were also analyzed. In order to determine the content of p-tyrosol and salidroside, the ultra performance liquid chromatography connected with a tandem mass spectrometry (UPLC-ESI MS/MS, Waters) was used. The obtained results shown that content of measured phenylethanoids depends on Rhodiola species. Roots of R. kirilowii contain more p-tyrosol, while R. rosea roots are reacher in salidroside. Our results indicate that the application of UPLC MS/MS method allows to determine the phenylethanoids content in tested samples with satisfactory precision.

PL

Przedmiotem badań było zwalidowanie metody analitycznej oznaczenia zawartości fenyloetanoidów. Do detekcji p-tyrozolu i salidrozydu w analizowanych dwóch gatunkach różeńca wykorzystano wysokosprawny chromatograf cieczowy sprzężony z tandemowym spektrometrem mas (UPLC-MS/MS). Analizie poddano Rhodiola kirilowii oraz Rhodiola rosea uzyskane z hodowli gruntowej w 2009 r. w Instytucie Włókien Naturalnych i Roślin Zielarskich w Poznaniu. Dodatkowo z surowca przygotowano dwa wyciągi suche: wyciąg wodny i wyciąg wodnoalkoholowy (50% wyciąg etanolowy). Zawartość poszczególnych składników różni się w zależności od analizowanej matrycy.

Słowa kluczowe

EN

phenylethanoid content; Rhodiola kirilowii; Rhodiola rosea; root; ultra performance liquid chromatography-tandem mass spectrometric method

• Wydawca: -
• Czasopismo: Herba Polonica
• Rocznik: 2012
• Tom: 58
• Numer: 4
• Opis fizyczny: p.29-39,fig.,ref.

Twórcy

autor

Gryszczynska A.

• Department of Quality Control of Medicinal Products and Dietary Supplements, Institute of Natural Fibres and Medicinal Plants, Libelta 27, 61-707 Poznan, Poland

autor

Lowicki Z.

• Department of Quality Control of Medicinal Products and Dietary Supplements, Institute of Natural Fibres and Medicinal Plants, Libelta 27, 61-707 Poznan, Poland

autor

Opala B.

• Department of Quality Control of Medicinal Products and Dietary Supplements, Institute of Natural Fibres and Medicinal Plants, Libelta 27, 61-707 Poznan, Poland

autor

Krajewska-Patan A.

• Department of Pharmacology and Experimental Biology, Institute of Natural Fibres and Medicinal Plants, Libelta 27, 61-707 Poznan, Poland

autor

Buchwald W.

• Team of Botany and Agriculture of Medicinal Plants, Department of Botany, Breeding and Agricultural Technology, Institute of Natural Fibres and Medicinal Plants, Kolejowa 2, 62-064 Plewiska/Poznan, Poland

autor

Czerny B.

• Department of General Pharmacology and Pharmacoeconomics, Pomeranian Medical University, Zolnierska 48, 70-204 Szczecin, Poland

autor

Mielcarek S.

• Department of Quality Control of Medicinal Products and Dietary Supplements, Institute of Natural Fibres and Medicinal Plants, Libelta 27, 61-707 Poznan, Poland

autor

Mrozikiewicz P. M.

• Laboratory of Experimental Pharmacogenetics, Department of Clinical Pharmacy and Biopharmacy, Poznan University of Medicinal Sciences, Sw.Marii Magdaleny 14, 61-861 Poznan, Poland

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