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2014 | 36 | 03 |

Tytuł artykułu

Altitudinal changes in the content of bioactive substances in Hypericum orientale and Hypericum pallens

Warianty tytułu

Języki publikacji



Altitudinal changes in the content of hypericin, pseudohypericin, hyperforin, adhyperforin, chlorogenic acid, neochlorogenic acid, caffeic acid, 2,4-Dihydroxybenzoic acid, amentoflavone, hyperoside, isoquercitrin, quercitrin, quercetin, avicularin, rutin, (+)-catechin and (-)-epicatechin among Hypericum orientale L. and Hypericum pallens Banks and Sol. populations from Northern Turkey were investigated for the first time. Thirty flowering individuals were collected from five different altitudes (400, 950, 1,150, 1,620 and 2,150 m) for H. pallens and six different altitudes (500, 1,150, 1,650, 2,100, 2,720 and 3,250 m) for H. orientale. The plant materials were dried at room temperature and subsequently assayed for chemical contents by HPLC. All chemicals were detected in both species at various levels depending on altitude of growing sites except for caffeic acid which was absent in H. pallens. It was found that plants from higher altitudes produced significantly higher amount of the bioactive compounds tested. The results were discussed as a possible protective response of plants to the different abiotic stress factors as high ultraviolet (UV)-B radiation and low temperature which were prevalent in higher altitudes.

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Opis fizyczny



  • Vocational High School of Bafra, Ondokuz Mayis University, 55400, Samsun, Bafra, Turkey
  • Institute of Botany, Nature Research Centre, Zaliuju ezeru 49, 08406, Vilnius, Lithuania
  • Medical Academy, Lithuanian University of Health Sciences, A. Mickeviciaus 9, 44307, Kaunas, Lithuania
  • Medical Academy, Lithuanian University of Health Sciences, A. Mickeviciaus 9, 44307, Kaunas, Lithuania
  • Vocational High School of Bafra, Ondokuz Mayis University, 55400, Samsun, Bafra, Turkey


  • Abreu IN, Mazzafera P (2005) Effect of water and temperature stress on the content of active constituents of Hypericum brasiliense Choisy. Plant Physiol Biochem 43:241–248
  • Albert A, Sareedenchai V, Heller W, Seidlitz HK, Zidorn C (2009) Temperature is the key to altitudinal variation of phenolics in Arnica montana L. cv. ARBO. Oecologia 160:1–8
  • Alonso-Amelot ME, Oliveros-Bastidas A, Calcagno-Pisarelli MP (2004) Phenolics and condensed tannins in relation to altitude in neotropical Pteridium spp. A field study in the Venezuelan Andes. Biochem Syst Ecol 32:969–981
  • Alonso-Amelot ME, Oliveros-Bastidas A, Calcagno-Pisarelli MP (2007) Phenolics and condensed tannins of high altitude Pteridium arachnoideum in relation to sunlight exposure, elevation, and rain regime. Biochem Syst Ecol 35:1–10
  • Bernal M, Llorens L, Julkunen-Tiitto R, Badosa J, Verdaguer D (2013) Altitudinal and seasonal changes of phenolic compounds in Buxus sempervirens leaves and cuticles. Plant Physiol Biochem 70:471–482
  • Bilger W, Rolland M, Nybakken L (2007) UV screening in higher plants induced by low temperature in the absence of UV-B radiation. Photochem Photobiol Sci 6:190–195
  • Bingol U, Cosge B, Gurbuz B (2011) Hypericum species in flora of Turkey. In: Odabas MS, Cirak C (eds) Hypericum. Medicinal and aromatic plant science and biotechnology 5 (Special issue 1):86–90
  • Blumthaler M, Ambach W, Ellinger R (1997) Increase in solar UV radiation with altitude. J Photochem Photobiol B 39:130–134
  • Chen Y, Jiang Y, Duan J, Shi J, Xue S, Kakuda Y (2010) Variation in catechin contents in relation to quality of ‘Huang Zhi Xiang’ Oolong tea (Camellia sinensis) at various growing altitudes and seasons. Food Chem 119:648–652
  • Cirak C, Radusiene J, Stanius Z, Camas N, Caliskan O, Odabas MS (2012) Secondary metabolites of Hypericum orientale L. growing in Turkey: variation among populations and plant parts. Acta Physiol Plant 34:1313–1320
  • Crockett SL, Robson NKB (2011) Taxonomy and chemotaxonomy of the genus Hypericum. In: Odabas MS, Çırak C (eds) Hypericum. Medicinal and aromatic plant science and biotechnology 5 (Special issue 1): 1–13
  • Demirci B, Baser KHC, Crockett SL, Khan IA (2005) Analysis of the volatile constituents of Asian Hypericum L. (Clusiaceae, Hyperidoideae) species. J Essent Oil Res 17:659–663
  • Dona M, Dell’Aica I, Pezzato E, Sartor L, Calabrese F, Della Barbera M, Donella-Deana A, Appendino G, Borsarini A, Caniato R, Garbisa S (2004) Hyperforin inhibits cancer invasion and metastasis. Cancer Res 64:6225–6232
  • Du H, Bay BH, Mahendran R, Olivo M (2006) Hypericin-mediated photodynamic therapy elicits differential interleukin-6 response in nasopharyngeal cancer. Cancer Lett 235:202–208
  • Feisst C, Werz O (2004) Suppression of receptor-mediated Ca2+ mobilization and functional leukocyte responses by hyperforin. Biochem Pharmacol 67:1531–1539
  • Fiebich BL, Knörle R, Apel K, Kammler T, Weiss G (2011) Pharmacological studies in an herbal drug combination of St. John’s wort (Hypericum perforatum) and passion flower (Passiflora incarnata): in vitro and in vivo evidence of synergy between Hypericum and Passiflora in antidepressant pharmacological models. Fitoterapia 82:474–480
  • Ganzera M, Guggenberger M, Stuppner H, Zidorn C (2008) Altitudinal variation of secondary metabolite profiles in flowering heads of Matricaria chamomilla cv. BONA. Planta Med 74:453–457
  • Gastpar M, Zeller K (2005) Hypericum-Extrakt STW3 und Sertralin zur Behandlung der mittelschweren depression. Psychopharmakotherapie 12:146–153
  • Germ M, Stibilj V, Kreft S, Gaberscik A, Kreft I (2010) Flavonoid, tannin and hypericin concentrations in the leaves of St. John’s wort (Hypericum perforatum L.) are affected by UV-B radiation levels. Food Chem 122:471–474
  • Guedes RC, Eriksson LA (2005) Theoretical study of hypericin. J Photochem Photobiol A Chem 172:293–299
  • Kasper S, Caraci F, Forti B, Drago F, Aguglia E (2010) Efficacy and tolerability of Hypericum extract for the treatment of mild to moderate depression. Eur Neuropsychopharm 20:74–765
  • Pharm Eur (2010) Directorate for the Quality of Medicine. European Pharmacopoeia 7th edn, Council of Europe, Strasbourg. 1241–1244
  • Rieger G, Muller M, Guttenberger H, Bucar F (2008) Influence of altitudinal variation on the content of phenolic compounds in wild populations of Calluna vulgaris, Sambucus nigra, and Vaccinium myrtillus. J Agric Food Chem 56:9080–9086
  • Schwarz D, Kisselev P, Roots I (2003) St. John’s wort extracts and some of their constituents potently inhibit ultimate carcinogen formation from benzo [a]pyrene-7,8-Dihydrodiol by human CYP1A1. Cancer Res 63:8062–8068
  • Sezik E, Yesilada E, Honda G, Takaishi Y, Takeda Y, Tanaka T (2001) Traditional medicine in Turkey X. Folk medicine in Central Anatolia. J Ethnopharmacol 75:95–115
  • Smelcerovic A, Zuehlke S, Spiteller M, Raabe N, Özen T (2008) Phenolic constituents of 17 Hypericum species from Turkey. Biochem Syst Ecol 36:316–319
  • Sommavilla V, Haidacher-Gasser D, Sgarbossa M, Zidorn C (2012) Seasonal variation in phenolics in leaves of Celtis australis (Cannabaceae). Biochem Syst Ecol 41:110–114
  • Spitaler R, Winkler A, Lins I, Yanar S, Stuppner H, Zidorn C (2008) Altitudinal variation of phenolic contents in flowering heads of Arnica montana cv. ARBO: a 3-year comparison. J Chem Ecol 34:369–375
  • Turkan I, Demiral T (2009) Recent developments in understanding salinity tolerance. Environ Exp Bot 67:2–6
  • Umek A, Kreft S, Kartnig T, Heydel B (1999) Quantitative phytochemical analysis of six Hypericum species growing in Slovenia. Planta Med 65:388–390
  • Xenophontos M, Stavropoulos I, Avramakis E, Navakoudis E, Dornemann D, Kotzabasis K (2008) Influence of the habitat altitude on the (proto)hypericin and (proto)pseudohypericin levels of Hypericum plants from Crete. Planta Med 74:1496–1503
  • Zhao M, Wang H, Yang B, Tao H (2010) Identification of cyclodextrin inclusion complex of chlorogenic acid and its antimicrobial activity. Food Chem 120:1138–1142
  • Zidorn C (2010) Altitudinal variation of secondary metabolites in flowering heads of the Asteraceae: trends and causes. Phytochem Rev 9:197–203
  • Zidorn C, Schubert B, Stuppner H (2005) Altitudinal differences in the contents of phenolics in flowering heads of three members of the tribe Lactuceae (Asteraceae) occurring as introduced species in New Zealand. Biochem Syst Ecol 33:855–872
  • Zlatev ZS, Lidon FCJ, Kaimakanoval M (2012) Plant physiological responses to UV-B radiation. Emir J Food Agric 24:481–501

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