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2013 | 22 | 2 |

Tytuł artykułu

The effect of LPS on LH release and gene expression of LH-beta, GnRH-R and TLR4 in the anterior pituitary of follicular phase ewes - an in vitro study

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The study was designed to determine the in vitro effect of bacterial endotoxin – lipopolysaccharide (LPS) – on luteinizing hormone (LH) secretion from anterior pituitary (AP) explants collected from saline-treated ‘healthy’ and LPS-treated ewes in the follicular phase. In the AP explants, the expression of LH-β, gonadotropin-releasing hormone receptor (GnRH-R) and Toll-like receptor 4 (TLR4) genes was also assayed. It was found that explants incubated alone with LPS, as well as together with LPS-binding protein (LBP), abolished the stimulatory effect of GnRH on LH release. LPS added without LBP did not suppress LH-β gene expression in ‘healthy’ APs, however. Moreover, LBP intensified the inhibitory effect of LPS on gene expression in ‘healthy’ APs. These results show that LPS is a potent negative modulator of LH secretion and suggest that its direct action on the pituitary gland could be one of the mechanisms via which an immune/inflammatory challenge inhibits the reproductive process.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

22

Numer

2

Opis fizyczny

p.97-105,fig.,ref.

Twórcy

autor
  • The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jablonna, Poland
autor
  • The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jablonna, Poland
  • The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jablonna, Poland

Bibliografia

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Typ dokumentu

Bibliografia

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Identyfikator YADDA

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