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2012 | 59 | 2 |
Tytuł artykułu

Reduction of bilirubin ditaurate by the intestinal bacterium Clostridium perfringens

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EN
Abstrakty
EN
 Bilirubin is degraded in the human gut by microflora into urobilinoids. In our study we investigated whether the bilirubin-reducing strain of Clostridium perfringens can reduce bilirubin ditaurate (BDT), a bile pigment of some lower vertebrates, without hydrolysis of the taurine moiety. C. perfringes was incubated under anaerobic conditions with BDT; reduction products were quantified by spectrophotometry and separated by TLC. Based on Rf values of BDT reduction products and synthetic urobilinogen ditaurate, three novel taurine-conjugated urobilinoids were identified. It is likely that bilirubin-reducing enzyme(s) serve for the effective disposal of electrons produced by fermentolytic processes in these anaerobic bacteria.
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-
Rocznik
Tom
59
Numer
2
Opis fizyczny
p.289-291,fig.,ref.
Twórcy
autor
  • Institute of Medical Biochemistry and Laboratory Diagnostics, 1st Faculty of Medicine, Charles University in Prague, Prague, Czech Republic
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autor
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Bibliografia
  • Fahmy K, Gray CH, Nicholson DC (1972) The reduction of bile pigments by faecal and intestinal bacteria. Biochim Biophys Acta 264: 85-97. 
  • Gustafsson BE, Lanke L (1960) Bilirubin and urobilins in germfree, ex-germfree, and conventional rats. J Exp Med 112: 975-981. 
  • Kotal P, Fevery J (1991) Quantitation of urobilinogen in feces, urine, bile and serum by direct spectrophotometry of zinc complex. Clin Chim Acta 202: 1-9. 
  • Moscowitz A, Weimer M, Lightner A, Petryka ZJ, Davis E, Watson CJ (1970) The in vitro conversion of bile pigments to the urobilinoids by a rat clostridia species as compared with the human fecal flora; III. Natural d-Urobilin, synthetic i-urobilin, and synthetic i-urobilinogen. Biochem Med 4: 149-164.  
  • Ridlon JM, Kang DJ, Hylemon PB (2006) Bile salt biotransformations by human intestinal bacteria. J Lipid Res 47: 241-259. 
  • Sakai T, Watanabe K, Kawatsu H (1987) Occurrence of ditaurobilirubin, bilirubin conjugated with two moles of taurine, in the gallbladder bile of yellowtail, Seriola quinqueradiata. J Biochem 102: 793-796. 
  • Vítek L, Ostrow JD (2009) Bilirubin chemistry and metabolism; harmful and protective aspects. Curr Pharm Des 15: 2869-2883. 
  • Vítek L, Kotal P, Jirsa M, Malina J, Černá M, Chmelař D, et al. (2000) Intestinal colonization leading to fecal urobilinoid excretion may play a role in the pathogenesis of neonatal jaundice. J Pediatr Gastroenterol Nutr 30: 294-298. 
  • Vítek L, Zelenka J, Zadinová M, Malina J (2005) The impact of intestinal microflora on serum bilirubin levels. J Hepatol 42: 238-243. 
  • Vítek L, Majer F, Muchová L, Zelenka J, Jirásková A, Branny P, et al. (2006) Identification of bilirubin reduction products formed by Clostridium perfringens isolated from human neonatal fecal flora. J Chromatogr B Analyt Technol Biomed Life Sci 833: 149-157. 
  • Watson CJ (1953) The direct preparation of crystalline urobilin from bilirubin. J Biol Chem 200: 691-696. 
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