Reduction of bilirubin ditaurate by the intestinal bacterium Clostridium perfringens

• Autorzy: Konickova R. , Jiraskova A. , Zelenka J. , Leseticky L. , Sticha M. , Vitek L.
• Języki publikacji: EN

Abstrakty

EN

 Bilirubin is degraded in the human gut by microflora into urobilinoids. In our study we investigated whether the bilirubin-reducing strain of Clostridium perfringens can reduce bilirubin ditaurate (BDT), a bile pigment of some lower vertebrates, without hydrolysis of the taurine moiety. C. perfringes was incubated under anaerobic conditions with BDT; reduction products were quantified by spectrophotometry and separated by TLC. Based on Rf values of BDT reduction products and synthetic urobilinogen ditaurate, three novel taurine-conjugated urobilinoids were identified. It is likely that bilirubin-reducing enzyme(s) serve for the effective disposal of electrons produced by fermentolytic processes in these anaerobic bacteria.

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2012
• Tom: 59
• Numer: 2
• Opis fizyczny: p.289-291,fig.,ref.

Twórcy

autor

Konickova R.

• Institute of Medical Biochemistry and Laboratory Diagnostics, 1st Faculty of Medicine, Charles University in Prague, Prague, Czech Republic

autor

Jiraskova A.

autor

Zelenka J.

autor

Leseticky L.

autor

Sticha M.

autor

Vitek L.

Bibliografia

• Fahmy K, Gray CH, Nicholson DC (1972) The reduction of bile pigments by faecal and intestinal bacteria. Biochim Biophys Acta 264: 85-97. 
• Gustafsson BE, Lanke L (1960) Bilirubin and urobilins in germfree, ex-germfree, and conventional rats. J Exp Med 112: 975-981. 
• Kotal P, Fevery J (1991) Quantitation of urobilinogen in feces, urine, bile and serum by direct spectrophotometry of zinc complex. Clin Chim Acta 202: 1-9. 
• Moscowitz A, Weimer M, Lightner A, Petryka ZJ, Davis E, Watson CJ (1970) The in vitro conversion of bile pigments to the urobilinoids by a rat clostridia species as compared with the human fecal flora; III. Natural d-Urobilin, synthetic i-urobilin, and synthetic i-urobilinogen. Biochem Med 4: 149-164.  
• Ridlon JM, Kang DJ, Hylemon PB (2006) Bile salt biotransformations by human intestinal bacteria. J Lipid Res 47: 241-259. 
• Sakai T, Watanabe K, Kawatsu H (1987) Occurrence of ditaurobilirubin, bilirubin conjugated with two moles of taurine, in the gallbladder bile of yellowtail, Seriola quinqueradiata. J Biochem 102: 793-796. 
• Vítek L, Ostrow JD (2009) Bilirubin chemistry and metabolism; harmful and protective aspects. Curr Pharm Des 15: 2869-2883. 
• Vítek L, Kotal P, Jirsa M, Malina J, Černá M, Chmelař D, et al. (2000) Intestinal colonization leading to fecal urobilinoid excretion may play a role in the pathogenesis of neonatal jaundice. J Pediatr Gastroenterol Nutr 30: 294-298. 
• Vítek L, Zelenka J, Zadinová M, Malina J (2005) The impact of intestinal microflora on serum bilirubin levels. J Hepatol 42: 238-243. 
• Vítek L, Majer F, Muchová L, Zelenka J, Jirásková A, Branny P, et al. (2006) Identification of bilirubin reduction products formed by Clostridium perfringens isolated from human neonatal fecal flora. J Chromatogr B Analyt Technol Biomed Life Sci 833: 149-157. 
• Watson CJ (1953) The direct preparation of crystalline urobilin from bilirubin. J Biol Chem 200: 691-696. 

Uwagi

PL

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