PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2011 | 51 | 1 |
Tytuł artykułu

Virulence of Alternaria alternata infecting noni associated with production of cell wall degrading enzymes

Warianty tytułu
PL
Wirulencja grzyba Alternaria alternata porażajacego drzewo Morinda citrifolia oraz wytwarzanie przez patogena enzymów degradyjących ściany komórkowe
Języki publikacji
EN
Abstrakty
EN
Different methods of inoculations were tried for proving Koch postulates. Among them, the pin prick plus spore suspension spray was found be the best method used in a glasshouse. Out of the fifteen isolates of Alternaria alternata, isolate AA1 was highly virulent and AA6 was avirulent. The virulent isolate of A. alternata produced more cellulolytic (C1 and Cx) and pectinolytic (macerating enzymes, pectin methyl esterase and endo-polygalacturonase) enzymes in vitro than the avirulent one. The activity of cellulolytic enzymes increased with the increase in the age of the culture. Whereas, the pectinolytic enzymes were highly active in 10-day-old culture and the activities decreased with the increase in the age of the culture. Then again, the activity of enzymes produced by the avirulent isolate of pathogens did not decrease and these enzyme activities increased with the increase in the age of the culture.
PL
Aby potwierdzić postulaty Kocha testowano różne metody inokulacji. Najlepsze wyniki uzyskano stosując metodę opryskiwania zawiesiną zarodników. Spośród 15 badanych izolatów grzyba Alternaria alternata, izolat AA1 wykazywał wysoki stopień wirulencji, podczas gdy izolat AA6 był awirulentny. Wirulentny izolat grzyba A. alternata wytwarzał w większych ilościach enzymy celulolityczne (C1 i Cx) oraz pektynolityczne (macerujące enzymy, pektyno metyl esterazę i endo-poligalakturonazę) in vitro, w porównaniu do wirulentnego izolatu. Enzymy pektolityczne były bardzo aktywne u 10-dniowych kultur grzyba, a ich aktywność malała wraz z wiekiem izolatu patogena. W przypadku enzymów wytwarzanych przez awirulentny izolat, stwierdzono wzrost ich aktywności wraz z wiekiem kultury grzyba.
Słowa kluczowe
EN
Wydawca
-
Rocznik
Tom
51
Numer
1
Opis fizyczny
p.87-92,fig.,ref.
Twórcy
autor
  • Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore-641003, India
autor
autor
autor
Bibliografia
  • Anand T. 2002. Studies on fruit rot of chilli (Capsicum annuum L.) caused by Colletotrichum capsici (Syd.) Butler and Bisby and Alternaria alternata (Fr) Keissler. M. Sc. (Ag.) thesis, Tamil Nadu Agric. Univ. Coimbatore, India, 157 pp.
  • Anand T., Bhaskaran R., Raguchander T., Karthikeyan G., Rajesh M., Senthilraja G. 2008. Production of cell wall degrading enzymes and toxins by Colletotrichum capsici and Alternaria alternata causing fruit rot of chillies. J. Plant Protection Res. 48 (4): 437-451.
  • Awasthi R. P., Kolte S. J. 1989. Variability in Alternaria brassicae affecting rape seed and mustard. Indian Phytopathol. 42, p. 863.
  • Bateman D. F. 1964. Cellulase and the Rhizoctonia disease of bean. Phytopathology 54 (11): 1372-1377.
  • Bateman D. F., Miller R. 1966. Pectic enzymes in tissue degradation. Annu. Rev. Phytopathol. 4: 119-146.
  • Bhaskaran R., Kandaswamy T. K. 1978. Production of a toxic metabolite by Alternaria helianthi in vitro and in vivo. Madras Agric. J. 65: 801-804.
  • Chenglin Y., William S. C., Carl E. S. 1996. Purification and characterization of polygalacturanase produced by Penicillium expansum in apple fruit. Phytopatholgy 86 (11): 1160-1166.
  • Gomez K. A., Gomez A. A. 1984. Statistical Procedure for Agricultural Research. Wiley, New York, 680 pp.
  • Goodman R. N., Kiraly Z., Zaitlin M. 1967. The Biochemistry and Physiology of Infectious Plant Disease. Co., Inc. Princeton, New Jersey, 354 pp.
  • Gupta T. 1970. In vitro and in vivo production of pectolytic and cellulolytic enzymes by four isolates of Colletotrichum. p. 197-207. In: "Physiology of Parasitism" (G. P. Agarwal, K. S. Bilgrami, eds.). Today and Tomorrow's, New Delhi, 284 pp.
  • Hancock J. G., Miller R. L., Lorbeer J. W. 1964. Pectinolytic and cellulolytic enzymes produced by Botrytis alli, Botrytis cinerea and Botrytis squamosa in vitro and in vivo. Phytopathology 54 (8): 928-931.
  • Jha D. K., Gupta D. P. 1988. Production of pectinolytic enzymes by Alternaria triticina. Indian Phytopathol. 41, p. 652.
  • Lakshmanan P., Jeyarajan R., Vidhyasekaran P. 1990. A boll rot of cotton caused by Corynespora cassiicola in Tamil Nadu, India. Phytoparasitica 18 (2): 171-173.
  • Mahadevan A. 1965. Production of protopectinase by Fusarium vasinfectum. Curr. Sci. 19, p. 566.
  • Marimuthu T., Bhaskaran R., Shanmugam N., Purushothaman D. 1974. In vitro production of cell wall splitting enzymes by Alternaria sesami. Labd. J. Sci. Tech. 12B: 26-28.
  • Mc Kinney H. H. 1923. A new system of grading plant diseases. J. Agric. Res. 26 (2): 195-218.
  • Muthulakshmi P. 1990. Studies on fruit rot of chilli (Capsicum annuum L.) caused by Alternaria tenuis Nees. M. Sc. (Ag.) thesis, Tamil Nadu Agric. Univ. Madurai, India, 139 pp.
  • Nelson S. C. 2006. Species profiles for Pacific island agroforestry, Morinda citrifolia (noni) http://www.traditionaltree.org., p. 18. http://www.traditionaltree.org
  • Norkrans B. 1950. Influence of cellulolytic enzymes from hyphomycetes on cellulose preparations of different crystallinity. Physiol. Plantarum 3 (3): 75-85.
  • Peter P. I. 2009. Energetic vibrations of noni. Noni Search 2009 - Fourth National Symposium. World Noni Research Foundation, Chennai, India, 25-26 October 2009, p. 18.
  • Rangaswami G. 1958. An agar blocks technique for isolating soil micro organisms with special reference to Pythiaceous fungi. Sci. Cult. 24, p. 85.
  • Riker A. J., Riker R. S. 1936. Introduction to Research on Plant Diseases. John Swift Co., St. Louis, Chicago, 117 pp.
  • Singh R. S. 2002. Introduction to Principles of Plant Pathology. 4th ed. Oxford IBH Publishing Co. Pvt. Ltd, New Delhi, 398 pp.
  • TNAU (Tamil Nadu Agricultural University). 1980. Score Chart for Crop Diseases. Coimbatore, India, 56 pp.
  • Wood R. K. S., Balio A., Graniti A. 1972. Phytotoxins in Plant Disease. Academic Press, New York, 530 pp.
  • Yang Z., Rogers L. M., Song Y., Guo W., Kolattukudy P. E. 2005. Homoserine and asparagines are host signals that trigger in planta expression of a pathogenesis gene in Nectria haematococca. Proc. Natl. Acad. Sci. USA 102: 4197-4202.
Uwagi
Rekord w opracowaniu
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-5493a329-3846-48b8-b29b-7a3dbe2c2f6b
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.