Virulence of Alternaria alternata infecting noni associated with production of cell wall degrading enzymes

• Autorzy: Hubballi M. , Sornakili A. , Nakkeeran S. , Anand T. , Raguchander T.

Warianty tytułu

PL

Wirulencja grzyba Alternaria alternata porażajacego drzewo Morinda citrifolia oraz wytwarzanie przez patogena enzymów degradyjących ściany komórkowe

• Języki publikacji: EN

Abstrakty

EN

Different methods of inoculations were tried for proving Koch postulates. Among them, the pin prick plus spore suspension spray was found be the best method used in a glasshouse. Out of the fifteen isolates of Alternaria alternata, isolate AA1 was highly virulent and AA6 was avirulent. The virulent isolate of A. alternata produced more cellulolytic (C1 and Cx) and pectinolytic (macerating enzymes, pectin methyl esterase and endo-polygalacturonase) enzymes in vitro than the avirulent one. The activity of cellulolytic enzymes increased with the increase in the age of the culture. Whereas, the pectinolytic enzymes were highly active in 10-day-old culture and the activities decreased with the increase in the age of the culture. Then again, the activity of enzymes produced by the avirulent isolate of pathogens did not decrease and these enzyme activities increased with the increase in the age of the culture.

PL

Aby potwierdzić postulaty Kocha testowano różne metody inokulacji. Najlepsze wyniki uzyskano stosując metodę opryskiwania zawiesiną zarodników. Spośród 15 badanych izolatów grzyba Alternaria alternata, izolat AA1 wykazywał wysoki stopień wirulencji, podczas gdy izolat AA6 był awirulentny. Wirulentny izolat grzyba A. alternata wytwarzał w większych ilościach enzymy celulolityczne (C1 i Cx) oraz pektynolityczne (macerujące enzymy, pektyno metyl esterazę i endo-poligalakturonazę) in vitro, w porównaniu do wirulentnego izolatu. Enzymy pektolityczne były bardzo aktywne u 10-dniowych kultur grzyba, a ich aktywność malała wraz z wiekiem izolatu patogena. W przypadku enzymów wytwarzanych przez awirulentny izolat, stwierdzono wzrost ich aktywności wraz z wiekiem kultury grzyba.

• Wydawca: -
• Czasopismo: Journal of Plant Protection Research
• Rocznik: 2011
• Tom: 51
• Numer: 1
• Opis fizyczny: p.87-92,fig.,ref.

Twórcy

autor

Hubballi M.

• Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore-641003, India

autor

Sornakili A.

autor

Nakkeeran S.

autor

Anand T.

autor

Raguchander T.

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