Immunohistochemical evaluation of E-cadherin expression in normal and neoplastic canine mammary glands

• Autorzy: Lopuszynski W. , Szczubial M. , Klimiuk P. , Smiech A. , Dabrowski R. , Bochniarz M.
• Języki publikacji: EN

Abstrakty

EN

E-cadherin is an adhesion molecule playing a crucial role in the maintenance of epithelial tissue architecture. Deterioration of its functions, resulting in the loosening of intercellular linkages, may be conducive to neoplasm progression and metastasis formation. The aim of this study was to provide an immunohistochemical evaluation of E-cadherin expression in 6 sections of a normal canine mammary gland and in 8 benign and 36 malignant epithelial neoplasms, as well as to determine the relationship between the level of E-cadherin expression and the histological type and grade of tumour. In malignant tumours, a decreased level of E-cadherin expression was observed when compared to a normal gland and benign tumours, which depends on the histological type and malignancy grade. Moreover, a progressive decrease in E-cadherin expression was observed in mammary carcinomas with an increasing malignancy grade. This suggests that decreasing E-cadherin expression may contribute to the differentiation of poorly-differentiated neoplastic cells to an invasive phenotype, and may be one of the factors triggering the metastatic cascade.

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2015
• Tom: 71
• Numer: 03
• Opis fizyczny: p.152-157,fig.,ref.

Twórcy

autor

Lopuszynski W.

• Department of Pathological Anatomy, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Gleboka 30, 20-612 Lublin, Poland

autor

Szczubial M.

• Department and Clinic of Animal Reproduction, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Gleboka 30, 20-612 Lublin, Poland

autor

Klimiuk P.

• Department of Pathological Anatomy, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Gleboka 30, 20-612 Lublin, Poland

autor

Smiech A.

• Department of Pathological Anatomy, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Gleboka 30, 20-612 Lublin, Poland

autor

Dabrowski R.

• Department and Clinic of Animal Reproduction, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Gleboka 30, 20-612 Lublin, Poland

autor

Bochniarz M.

• Department and Clinic of Animal Reproduction, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Gleboka 30, 20-612 Lublin, Poland

Bibliografia

• 1. Arnesen, K., Gamlem H., Glattre E., Grondalen J., Moe L., Nordstoga K.: The Norwegian Canine Cancer Register 1990-1998. Report from the project “cancer in the dog”. EJCAP 2001, 11, 159-169.
• 2. Brønden L. B., Nielsen S. S., Toft N., Kristensen A. T.: Data from the Danish veterinary cancer registry on the occurrence and distribution of neoplasms indogs in Denmark. Vet. Rec. 2010, 166, 586-590.
• 3. Brunetti B., Sarli G., Preziosi R., Leprotti S., Benazzi C.: E-cadherin expression in canine mammary carcinoma with regional lymph node metastases. J. Vet.Med. A Physiol. Pathol. Clin. Med. 2003, 50, 496-500.
• 4. Cavallaro U., Christofori G.: Cell adhesion and signaling by cadherins and Ig-CAMs in cancer. Nat. Rev. Cancer 2004, 4, 118-132.
• 5. Chekhun S., Bezdenezhnykh N., Shvets J., Lukianova N.: Expression of biomarkers related to cell adhesion, metastasis and invasion of breast cancer celllines of different molecular subtype. Exp. Oncol. 2013, 35, 174-179.
• 6. Elston C. W., Ellis I. O.: Assessment of histological grade, [in:] Elston C. W. and Ellis I. O. (eds): The breast. Vol. 13. Churchill Livingstone, Edinburgh, New York 1998, p. 356-384.
• 7. Elston C. W., Ellis I. O., Pinder S. E.: Pathological prognostic factors in breast cancer. Crit. Rev. Oncol. Hematol. 1999, 31, 209-223.
• 8. Gama A., Paredes J., Gartner F., Alves A., Schmitt F.: Expression of E-caherin, P-cadherin and β-catenin in canine malignant mammary tumours in relationto clinico-pathological parameters, proliferation and survival. Vet. J. 2008,177, 45-53.
• 9. Halbleib J. M., Nelson W. J.: Cadherins in development: cell adhesion, sorting, and tissue morphogenesis. Genes. Dev. 2006, 20, 3199-3214.
• 10. Heimann R., Lan F., McBride R., Hellman S.: Separating favorable from unfavorable prognostic markers in breast cancer: the role of E-cadherin. CancerRes. 2000, 60, 298-304.
• 11. Jeschke U., Mylonas I., Kuhn C., Shabani N., Kunert-Keil C., Schindlbeck C., Gerber B., Friese K.: Expression of E-cadherin in human ductal breast cancercarcinoma in situ, invasive carcinomas, their lymph node metastases, theirdistant metastases, carcinomas with recurrence and in recurrence. AnticancerRes. 2007, 27, 1969-1974.
• 12. Li D. M., Feng Y. M.: Signaling mechanism of cell adhesion molecules in breast cancer metastasis: potential therapeutic targets. Brest Cancer Res. Treat. 2011,128, 7-21.
• 13. Matos A. J., Lopes C., Carvalheira J., Santos M., Rutteman G. R., Gartner F.: E-cadherin expression in canine malignant mammary tumours: relationshipto other clinico-pathological variables. J. Comp. Pathol. 2006, 134, 182-189.
• 14. Matos A. J. F. de, Lopes C. C., Faustino A. M., Carvalheira J. G., Rutteman G. R., Gartner M. F.: E-cadherin, β-catenin, invasion and lymph node metastasesin canine malignant mammary tumours. APMIS 2007, 115, 327-334.
• 15. Merlo D. F., Rossi L., Pellegrino C., Ceppi M., Cardellino U., Capurro C., Ratto A., Sambucco P. L., Sestito V., Tanara G., Bocchini V.: Cancer incidencein pet dogs: findings of the Animal Tumor Registry of Genoa, Italy. J. Vet.Intern. Med. 2008, 22, 976-984.
• 16. Misdorp W.: Tumors of the mammary gland, [in:] Meuten D. J. (ed.): Tumors in domestic animals, 4th ed. Iowa State Press, 2002, p. 575-606.
• 17. Misdorp W., Else R. W., Hellmen E., Lipscomb T. P.: Histological classification of the mammary tumours of the dog and the cat, [in:] Shulman F. I. (ed.): WorldHealth Organization International Histological Classification of Tumours ofDomestic Animals. Armed Forces Intitute of Pathology, Washington DC 1999,p. 16-29.
• 18. Nowak M., Madej J. A., Caputa R., Poradowski D.: Manifestation of tumours in domestic animals in Lower Silesia in 2005-2008. Bull. Vet. Inst. Pulawy2010, 54, 229-236.
• 19. Nowak M., Madej J. A., Dzięgiel P.: Expression of E-cadherin, β-catenin and Ki-67 antigen and their reciprocal relationships in mammary adenocarcinomasin bitches. Folia Histochem. Cytobiol. 2007, 45, 233-238.
• 20. Ohene-Abuakwa Y., Pignatelli M.: Adhesion molecules as diagnostic tools in tumor pathology. Int. J. Surg. Pathol. 2000, 8, 191-200.
• 21. Pang H., Lu H., Song H., Meng Q., Zhao Y., Liu N., Lan F., Liu Y., Yan S., Dong X., Cai L.: Prognostic values of osteopontin-c, E-cadherin and β-cateninin breast cancer. Cancer Epidemiol. 2013, 37, 985-992.
• 22. Park D., Karesen R., Axcrona U., Noren T., Sauer T.: Expression pattern of adhesion molecules (E-cadherin, α-, β-, γ-catenin and claudin-7), their influenceon survival in primary breast carcinoma, and their corresponding axillarylymph node metastasis. APMIS 2007, 115, 52-65.
• 23. Parker C., Rampaul R. S., Pinder S. E., Bell J. A., Wencyk P. M., Blamey R. W., Nicholson R. I., Robertson J. F. R., Ellis I. O.: E-cadherin as a prognostic indicator in primary breast cancer. Br. J. Cancer 2001, 85, 1958-1963.
• 24. Perl A. K., Wilgenbus P., Dahl U., Semb H., Christofory G.: A causal role for E-cadherin in the transition from adenoma to carcinoma. Nature 1998, 392, 190-193.
• 25. Qureshi H. S., Linden M. D., Divine G., Raju U. B.: E-cadherin status in breast cancer correlates with histologic type but does not correlate with establishedprognostic parameters. Am. J. Clin. Pathol. 2006, 125, 377-385.
• 26. Reis A. L., Carvalheira J., Schmidt F. C., Gartner F.: Immunohistochemical study of the expression of E-cadherin in canine mammary tumours. Vet. Rec.2003, 152, 621-624.
• 27. Reis-Filho J. S., Paredes J., Milaneza F., Schmitt F. C.: Clinicopathological implications of E-cadherin reactivity in patients with lobular carcinoma insitu of the breast. Cancer 2002, 94, 2114-2115.
• 28. Remmele W., Stegner H. E.: Recommendation for uniform definition of an immunoreactive score (IRS) for immunohistochemical estrogen receptordetection (ER-ICA) in breast cancer tissue. Pathologe 1987, 8, 138-140.
• 29. Restucci B., Papparella S., De Vico G., Maiolino P.: E-cadherin expression in normal and neoplastic canine mammary gland. J. Comp. Pathol. 1997, 116,191-202.
• 30. Rodo A., Malicka E.: E-cadherin immunohistochemical expression in mammary gland neoplasms in bitches. Pol. J. Vet. Sci. 2008, 11, 47-54.
• 31. Singhai R., Patil V. W., Jaiswal S. R., Patil S. D., Tayade M. B., Patil A. V.: E-Cadherin as a diagnostic biomarker in breast cancer. N. Am. J. Med. Sci.2011, 3, 227-233.
• 32. Wijnhoven B. P., Tucker E. T., Dinjens W. N., Tilanus H. W., Pignatelli M.: Biochemical analysis and subcellular distribution of E-cadherin-catenin inadenocarcinomas of the gastro-oesophageal junction. Anticancer Res. 2004,24, 1369-1375.
• 33. Zhang H., Liu J., Yue D., Gao L., Wang D., Zhang H., Wang C.: Clinical significance of E-cadherin, β-catenin, vimentin and S100A4 expression incompletely resected squamous cell lung carcinoma. J. Clin. Pathol. 2013, 66,937-945.