Characterization of mutations causing rifampicin and isoniazid resistance of Mycobacterium tuberculosis in Syria

• Autorzy: Madania A. , Habous M. , Zarzour H. , Ghoury I. , Hebbo B.
• Języki publikacji: EN

Abstrakty

EN

In order to characterize mutations causing rifampicin and isoniazid resistance of M. tuberculosis in Syria, 69 rifampicin resistant (Rifr) and 72 isoniazid resistant (Inhr) isolates were screened for point mutations in hot spots of the rpoB, katG and inhA genes by DNA sequencing and real time PCR. Of 69 Rifr isolates, 62 (90%) had mutations in the rifampin resistance determining region (RRDR) of the rpoB gene, with codons 531 (61%), 526 (13%), and 516 (8.7%) being the most commonly mutated. We found two new mutations (Asp516Thr and Ser531Gly) described for the first time in the rpoB-RRDR in association with rifampicin resistance. Only one mutation (Ile572Phe) was found outside the rpoB-RRDR. Of 72 Inhr strains, 30 (41.6%) had a mutation in katGcodon 315 (with Ser315Thr being the predominant alteration), and 23 (32%) harbored the inhA−15C→T mutation. While the general pattern of rpoB-RRDR and katG mutations reflected those found worldwide, the prevalence of the inhA−15C→T mutation was above the value found in most other countries, emphasizing the great importance of testing the inhA−15C→T mutation for prediction of isoniazid resistance in Syria. Sensitivity of a rapid test using real time PCR and 3’-Minor groove binder (MGB) probes in detecting Rifr and Inhr isolates was 90% and 69.4%, respectively. This demonstrates that a small set of MGB-probes can be used in real time PCR in order to detect most mutations causing resistance to rifampicin and isoniazid.

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2012
• Tom: 61
• Numer: 1
• Opis fizyczny: p.23-32,fig.,ref.

Twórcy

autor

Madania A.

• Section of Medical Biology, Department of Radiation Medicine, Atomic Energy Commission, P.O. 6091, Damascus, Syria

autor

Habous M.

autor

Zarzour H.

autor

Ghoury I.

autor

Hebbo B.

Bibliografia

• Baker L.V., T.J. Brown, O. Maxwell, A.L. Gibson, Z. Fang, M.D. Yates and F.A. Drobniewski. 2005. Molecular analysis of isoniazid- resistant Mycobacterium tuberculosis isolates from England and Wales reveals the phylogenetic significance of the ahpC-46A polymorphism. Antimicrob. Agents Chemother. 49: 41455–1464.
• Bakonyte D., A. Baranauskaite, J. Cicenaite, A. Sosnovskaja and P. Stakenas. 2003. Molecular characterization of isoniazid-resistant Mycobacterium tuberculosis clinical isolates in Lithuania. Antimicrob. Agents Chemother. 47: 2009–2011.
• Bobadilla-del-Valle M., A. Ponce-de-Leon, C. Arenas-Huertero, G. Vargas-Alarcon, M. Kato-Maeda, P.M. Small, P. Couary, G.M. Ruiz-Palacios and J. Sifuentes-Osornio. 2001. rpoB gene mutations in rifampin-resistant Mycobacterium tuberculosis identified by polymerase chain reaction single-stranded conformational polymorphism. Emer. Infect. Dis. 7: 1010–1013.
• Cavusoglu C., S. Hilmioglu, S. Guneri and A. Bilgic. 2002. Characterization of rpoB mutations in rifampin-resistant clinical isolates of Mycobacterium tuberculosis from Turkey by DNA sequencing and line probe assay. J. Clin. Microbiol. 40: 4435–4438.
• Dye C. 2006. Global epidemiology of tuberculosis. Lancet 367: 938–940.
• Ederth J., R. Mooney, L. Isaksson and R. Landick. 2006. Functional interplay between the Jaw Domain of bacterial RNA polymerase and allele-specific residues in the product RNA-binding pocket. J. Mol. Biol. 356: 1163–1179.
• Fressatti Cardoso R., R. Cooksey, G. Morlock, P. Barco, L. Cecon, F. Forestiero, C.Q.F. Leite, D.N. Sato, M. de Lourdes Shikama, E.M. Mamizuka and others. 2004. Screening and characterization of mutations in isoniazid-resistant Mycobacterium tuberculosis isolates obtained in Brazil. Antimicrob. Agents Chemother. 48: 3373–3381.
• Gagneux S. 2009. Fitness cost of drug resistance in Mycobacterium tuberculosis. Clin. Microbiol. Infect. 15:66–68.
• García de Viedma D. 2003. Rapid detection of resistance in Mycobacterium tuberculosis: a review discussing molecular approaches. Clin. Microbiol. Infect. 9: 349–359.
• Hazbón M.H., M. Brimacombe, M. Bobadilla del Valle, M. Cavatore, M. Inírida Guerrero, M. Varma-Basil, H. Billman-Jacobe, C. Lavender, J. Fyfe, L. Garcia-Garcia and others. 2006. Population genetics study of isoniazid resistance mutations and evolution of multidrug resistant Mycobacterium tuberculosis. Antimicrob. Agents Chemother. 50: 2640–2649.
• Heep M., B. Brandstatter, U. Rieger, N. Lehn, E. Richter, S. Rusch-Gerdes and S. Niemann. 2001. Frequency of rpoB mutations inside and outside the cluster I region in rifampin-resistant clinical Mycobacterium tuberculosis isolates. J. Clin. Microbiol. 39: 107–110.
• Herrera L., S. Jimenez, A. Valcerde, M.A. Garcia-Aranda and J.A. Saez-Nieto. 2003. Molecular analysis of rifampicin-resistant Mycobacterium tuberculosis isolated in Spain (1996–2001). Description of new mutations in the rpoB gene and review of the literature. Int. J. Antimicrob. Agents. 21: 403–408.
• Jiao W., I. Mokrousov, G. Sun, M. Li, J. Liu, O. Narvskaya and A. Shen. 2007. Molecular characteristics of rifampin and isoniazid resistant Mycobacterium tuberculosis strains from Beijing, China. Chin. Med. J. 120: 814–819.
• Kutyavin I.V., I.A. Afonina, A. Mills, V.V. Gorn, E.A. Lukhtanov, E.S. Belousov, M.J. Singer, D.K. Walburger, S.G. Lokhov, A.A. Gall and others. 2000. 3’-Minor groove binder-DNA probes increase sequence specificity at PCR extension temperatures. Nucleic Acids Res. 28: 655–661.
• Mani C., N. Selvakumar, S. Narayanan and P.R. Narayanan. 2001. Mutations in the rpoB gene of multidrug-resistant Mycobacterium tuberculosis clinical isolates from India. J. Clin. Microbiol. 39: 2987–2990.
• McCammon M., J. Gillette, P. Thomas, V. Ramaswamy, A. Graviss, N. Kreiswirth, J. Vijg and N. Quitugua. 2005. Detection of rpoB mutations associated with rifampin resistance in Mycobacterium tuberculosis using denaturing gradient gel electrophoresis. Antimicrob. Agents Chemother. 49: 2200–2209.
• Narvaiz de Kantor I., S.J. Kim, T. Frieden, A. Laszlo, F. Luelmo, P.Y. Norval, H. Rieder, P. Valenzuela and K. Weyer. 1998. LABORATORY SERVICES IN TUBERCULOSIS CONTROL, For the Global Tuberculosis Programme, World Health Organization, Geneva, Switzerland.
• Ohno H., H. Koga, S. Kohno, T. Tashiro, and K. Hara. 1996. Relationship between rifampin MICs and rpoB mutations of Mycobacterium tuberculosis strains isolated in Japan. Antimicrob. Agents Chemother. 40: 1053–1056.
• Pozzi G., M. Meloni, E. Iona, G. Orro, O.F. Thorensen, M.L. Ricci, M.R. Oggioni, L. Fattorini and G. Orefici. 1999. rpoB mutations in multidrug resistant strains of Mycobacterium tuberculosis isolated in Italy. J. Clin. Microbiol. 37:1197–1199.
• Ramaswamy S.V. and J.M. Musser. 1998. Molecular genetic basis of antimicrobial agent resistance in Mycobacterium tuberculosis: 1998 update. Tuberc. Lung. Dis. 79:3–29.
• Ramaswamy S.V., R. Reich, S.J. Dou, L. Jasperse, X. Pan, A. Wanger, T. Quitugua and E.A. Graviss. 2003. Single nucleotide polymorphisms in genes associated with isoniazid resistance in Mycobacterium tuberculosis. Antimicrob. Agents Chemother. 47: 1241–1250.
• Rouse, D. A., J. A. DeVito, Z. Li, H. Byer, and S. L. Morris. 1996. Site directed mutagenesis of the katG gene of Mycobacterium tuberculosis: effects on catalase-peroxidase activities and isoniazid resistance.Mol. Microbiol. 22: 583–592.
• Silva M.S., S.G. Senna, M.O. Ribeiro, A.R. Valim, M.A. Telles, A. Kritski, G.P. Morlock, R.C. Cooksey, A. Zaha and M.L. Rossetti. 2003. Mutations in katG, inhA, and ahpC genes of Brazilian isoniazid-resistant isolates of Mycobacterium tuberculosis. J. Clin. Microbiol. 41: 4471–4474.
• Slayden R.A. and C.E. Barry 3rd. 2000. The genetics and biochemistry of isoniazid resistance in Mycobacterium tuberculosis. Microbes Infect. 2: 659–669.
• Telenti A., N. Honore, C. Bernasconi, J. March, A. Ortega, B. Heym, H.E. Taki& and S.T. Cole. 1997. Genotypic assessment of isoniazid and rifampin resistance in Mycobacterium tuberculosis: a blind study at reference laboratory level. J. Clin. Microbiol. 35: 719–723.
• van Soolingen D., P.E. de Haas, H.R. van Doorn, E. Kuijper, H. Rinder and M.W. Borgdor &. 2000. Mutations at amino acid position 315 of the katG gene are associated with high-level resistance to isoniazid, other drug resistance, and successful transmission of Mycobacterium tuberculosis in the Netherlands. J. Infect. Dis. 182: 1788–1790.
• Wada T., S. Maeda, A. Tamaru, S. Imai, A. Hase and K. Kobayashi. 2004. Dual-probe assay for rapid detection of drug-resistant Myco bacterium tuberculosis by real-time PCR. J. Clin. Microbiol. 42: 5277–5285.
• Wei C.J., B. Lei, J.M. Musser and S.C. Tu. 2003. Isoniazid activation defects in recombinant Mycobacterium tuberculosis catalaseperoxidase (KatG) mutants evident in InhA inhibitor production. Antimicrob. Agents Chemother. 47: 670–675.
• World Health Organization. 2005. Global tuberculosis control: surveillance, planning, financing. WHO report 2005. WHO/HTM/ TB/2005.349. Geneva.
• World Health Organization. 2010. Multidrug and extensively drug resistant TB (M/XDR-TB): 2010 global report on surveillance and response. WHO/HTM/TB/2010.3. Geneva, Switzerland.
• Yuen L.K.W., D. Leslie and P.J. Coloe. 1999. Bacteriological and molecular analysis of rifampin-resistant Mycobacterium tuberculosis strains isolated in Australia. J. Clin. Microbiol. 37: 3844–3850.
• Zhang Y. and A. Telenti. 2000. Genetics of drug resistance in Mycobacterium tuberculosis, p. 235–254. In Hatfull F.F and Jacobs W.R Jr. (ed.), Molecular genetics of mycobacteria. ASM Press, Washington, D.C.
• Zhang M., J. Yue, Y.P. Yang, H.M. Zhang, J.Q. Lei, R.L. Jin, X.L. Zhang and H.H. Wang. 2005. Detection of mutations associated with isoniazid resistance in Mycobacterium tuberculosis isolates from China. J. Clin. Microbiol. 43: 5477–5482

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