Phylogenetic analysis of Nosema apis and Nosema ceranae small subunit 16S rRNA in honey bees (Apis mellifera) from north-eastern Poland

• Autorzy: Michalczyk M. , Sokol R. , Szczerba-Turek A.
• Języki publikacji: EN

Abstrakty

EN

In order to perform a phylogenetic analysis of Nosema spp. spores, samples of 10 worker bees each were collected from 30 infected colonies (the presence of spores was confirmed by light microscopy) kept in 15 apiaries in north-eastern Poland. Both N. apis and N. ceranae are common in this region (mixed infection N. apis/ N. ceranae – 60%, N. ceranae – 37%, N. apis – 3%). The DNA samples of N. apis were 100% identical with the N. apis sequences deposited in the GenBank database in Queensland, Australia, Spain, New Zealand, Lithuania and Tasmania in Australia. The DNA samples of N. ceranae were found to be 99.5%-100% identical with the N. ceranae sequences previously published in Italy, Germany, Switzerland and Austria.

Słowa kluczowe

EN

phylogenetic analysis; Nosema apis; Nosema ceranae; rRNA; honey bee; Apis mellifera; animal disease; parasitic disease; nosemosis; Poland

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2013
• Tom: 69
• Numer: 12
• Opis fizyczny: p.733-735,fig.,ref.

Twórcy

autor

Michalczyk M.

• Department of Parasitology and Invasive Disease, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland

autor

Sokol R.

• Department of Parasitology and Invasive Disease, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland

autor

Szczerba-Turek A.

• Department of Epizootiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland

Bibliografia

• 1. Altschul S. F., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J.: Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 1997, 25, 3389-3402.
• 2. Cavalier-Smith T.: A revised six – kingdom system of life. Biol. Rev. 1998, 73, 203-266.
• 3. Chaimanee V., Chen Y., Pettis J. S., Cornman R. S., Chantawannakul P.: Phylogenetic analysis of Nosema ceranae isolated from European and Asian honeybees in Northern Thailand. J. Invertebr. Pathol. 2011, 107, 229-233.
• 4. Chen Y. P., Evans J. D., Murphy C., Gutell R., Zuker M., Gundensen-Rindal D., Pettis J. S.: Morphological, molecular, and phylogenetic characterization of Nosema ceranae, a microsporidian parasite isolated from the European honey bee, Apis mellifera. J. Eukaryot. Microbiol. 2009, 56, 142-147.
• 5. Chen Y. P., Huang Z. Y.: Nosema ceranae, a newly identified pathogen of Apis mellifera in the USA and Asia. Apidologie 2010, 41, 364-374.
• 6. Fries I.: Nosema ceranae in European honey bees (Apis mellifera). J. Invertebr. Pathol. 2010, 103, 73-79.
• 7. Gisder S., Möckel N., Linde A., Genersch E.: A cell culture model for Nosema ceranae and Nosema apis allows new insights into the life cycle of these important honey bee-pathogenic microsporidia. Environ. Microbiol. 2011, 13, 404-413.
• 8. Higes M., Garcia-Palencia P., Martin-Hernandez R., Meana A.: Experimental infection of Apis mellifera honeybees with Nosema ceranae (Microsporidia). J. Invertebr. Pathol. 2007, 94, 211-217.
• 9. Higes M., Martin-Hernández R., Meana A.: Nosema ceranae, a new microsporidian parasite in honeybees in Europe. J. Invertebr. Pathol. 2006, 92, 93-95.
• 10. Klee J., Beasana A. M., Genersch E., Gisder S., Nanetti A., Tam D. Q., Chinh T. X., Puerta F., Ruz J. M., Kryger P., Message D., Hatjina F., Korpela S., Fries I., Paxton R. J.: Widespread dispersal of the microsporidian Nosema ceranae an emergent pathogen of the western honey bee, Apis mellifera. J. Invertebr. Pathol. 2007, 96, 1-10.
• 11. Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A., Lopez R., Thompson J. D., Gibson T. J., Higgins D. G.: Clustal W and Clustal X version 2.0. Bioinformatics. 2007, 23, 2947-2948.
• 12. Medici S. K., Sarlo E. G., Porrini M. P., Braunstein M., Eguaras M. J.: Genetic variation and widespread dispersal of Nosema ceranae in Apis mellifera apiaries from Argentina. Parasitol Res. 2012, 110, 859-864.
• 13. Michalczyk M., Sokół R., Szczerba-Turek A., Bancerz-Kisiel A.: A comparison of effectiveness of the microscopic and the multiplex PCR method in identifying and discriminating the species of Nosema spp. spores in worker bees (Apis mellifera) from winter hive debris. Pol. J. Vet. Sci. 2011, 14, 385-391.
• 14. Paxton R., Klee J., Korpela S., Fries I.: Nosema ceranae has infected Apis mellifera in Europe since at least 1998 and may be more virulent than Nosema apis. Apidologie 2007, 38, 558-565.
• 15. Sina M., Alastair G., Farmer M., Andersen R., Anderson O., Barta J., Bowser S., Brugerolle G., Fensome R., Fredericq S., James T., Karpov S., Kugrens P., Krug J., Lane C., Lewis L., Lodge J., Lynn D., Mann D., Maccourt R., Mendoza L., Moestrup O., Mozley S., Nerad T., Shearer C., Smirnov A., Spiegel F., Taylor M.: The New Higher level classification of Eukaryotes with emphasis on the taxonomy of Protists. J. Eukar. Microbiol. 2005, 52, 399-451.
• 16. Sneath P. H. A., Sokal R. R.: Numerical taxonomy; the principles and practice of numerical classification. San Francisco, WH Freeman 1973, 230-234.
• 17. Tamura K., Nei M., Kumar S.: Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc. Natl. Acad. Sci. 2004, 101, 11030-11035.
• 18. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.: MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Mol. Biol. Evol. 2011, 28, 2731-2739.