Survival of BPV and Aujeszky's disease viruses in meat wastes subjected to different sanitization processes
Treść / Zawartość
The effect of composting and anaerobic fermentations under meso- and thermophylic conditions (37° and 55℃) on the survival of bovine parvovirus (BPV) and Aujeszky’s disease viruse (ADV) in meat wastes has been examined in this study. Viruses were adsorbed on filters and introduced into carriers which were made of meat fragments of different sizes and bones or in the form of suspension they were introduced into the biomass in the course of processes of waste treatment. Carriers were removed at appropriate time intervals and virus titres were determined. The thermoresistant parvovirus survived for the longest time during mesophylic fermentation (almost 70 days), slightly shorter during composting (7-9.5 days depending on the type of carrier) and for the shortest time – at 55℃ (46-76 hours). Its inactivation rate was the fastest in a suspension, slower in meat and bone carriers. ADV inactivation proceeded considerably faster, as compared with BPV. Its active particles were not detected as early as in the 30th minute of thermophylic fermentation, the 6th hour of mesophylic fermentation and at the first sampling time during composting (at the 72nd hour). Total survival time ranged from 50 min to 13 hours. All the tested technologies enabled the effective elimination of ADV and on average twofold decrease in BPV titre. From the study conducted it follows that of both viruses, the BPV should be applied for validation processes of methods used in meat waste processing, particularly if this refers to methods where higher temperature is the factor inactivating pathogens.
- Biermann U, Herbst W, Krauss H, Schliesser T (1989) Electron microscopic detection rate of enteral viruses in diarrhea of dogs, cats, calves, swine and foals in the year 1988 – electron microscopic study results. Berl Munch Tierarztl Wochenschr 102: 412-414.
- Bøtner A (1991) Survival of Aujeszky’s disease virus in slurry at various temperatures. Vet Microb 29: 225-235.
- Hoferer M (2001) Seuchenhygienische Untersuchungen zur inactivierung ausgewahlter Bakterien und Viren bei der mesophilen und termophilen anaetoben alkalischen Faulung von Biound Kuchenabfallen sowie anderen rest- und Abfallstoffen Tierischer Herkunft. D 188 (Dissertation Freie Universitat Berlin). Shaker Verlag, Aachen.
- Kärber G (1931) Beitrag zur kollektiven behandlung pharmakologischer reihenversuche. Arch Exp Pathol Pharmacol. 162: 480-488.
- Ligocka A, Paluszak Z (2008) Evaluation of Meat Waste Composting Process Based on Fecal Streptococci Survival. Polish J Environ Stud 17: 739-744.
- Lipowski A, Pejsak Z (1996) Aujeszky’s disease – known and unknown. Medycyna Wet 52: 490-494.
- Lund E, Nissen B (1983) The survival of enteroviruses in aerated and unareated cattle and pig slurry. Agr Wastes 7: 221-233.
- Monteith HD, Shannon EE (1986) The inactivation of a bovine enterovirus and a bovine parvovirus in cattle manure by anaerobic digestion, heat treatment, gamma irradiation, ensilage and composting. J Hyg 97: 175-184.
- Nørrung B, Buncic S (2008) Microbial safety of meat in the European Union. Meat Sci 78: 14-24.
- Olszewska H, Paluszak Z, Jarząbek Z (2008) Survival of bovine enterovirus strain LCR-4 in water, slurry, and soil. Bull Vet Inst Pulawy 52: 205-209.
- Pejsak Z, Truszczyński MJ (2006) Aujeszky’s Disease (Pseudorabies). In: Straw BE, Zimmerman JJ, D’Allaire S, Taylor J (eds) Diseases of Swine, 9th ed., Blackwell Publishing, Ames, IA, pp. 419-433.
- European Parliament, Council, COD 2000/0259 Regulation No. 1774/2002 laying down health rules concerning animal by-products not intended for human consumption (www.eur-lex.europa.eu).
- Rutishauser UP, Metzler AE, Corboz L, Wyler R (1984) Incidence of virus isolation in fecal samples of swine with diarrhea including steatorrhea. Schweiz Arch Tierheilkd 126: 453-466.
- Straub TM, Pepper IL, Gerba CP (1993) Hazards from pathogenic microorganisms in land-disposed sewage sludge. Rev Environ Contam Toxicol 132: 55-91.
- Traub F, Spillmann SK, Wyler R (1986) Method for determining virus inactivation during sludge treatment processes. Appl Environ Microbiol 52(3): 498-503.
- Turner C, Williams SM, Cumby TR (2000) The inactivation of foot and mouth disease, Aujeszky’s disease and classical swine fever viruses in pig slurry. J Appl Microbiol 89: 760-767.
- Wekerle J, Albrecht H (1983) Inactivation of vaccinia virus and a bovine enterovirus in aerated pig slurry with special regard to pH, temperature and free ammonia modification during aeration. Agr Wastes 7: 39-50.