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2012 | 56 | 3 |

Tytuł artykułu

Effect of 2-methoxyestradiol on gonadotropin secretion and oxidative status of porcine pituitary cells in vitro

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The aim of the study was to investigate the effect of 2-methoxyestradiol (2-ME) on GnRH-induced LH and FSH secretion by porcine pituitary cells in vitro. Moreover, the concentrations of superoxide anion radical (O₂⁻), as a possible mediator of 2-ME action, and malonyldialdehyde (MDA), as an oxidative stress indicator, were estimated. Pituitary cells were cultured in McCoy 5A medium with GnRH (positive control), with GnRH and 3.3× l0⁻¹¹,-3.3×10⁻⁷ M/L of 2-ME, or with GnRH and 2.7×10⁻⁹- 2.7×10⁻⁸ M/L of 17ß-estradiol. The secretion of gonadotropins, as well as concentrations of superoxide anion and MDA were analysed after 2-72 h of the experiment. A positive correlation between 2-ME dose and FSH secretion from anterior pituitary cells (r=0.72, 0.95, 0.92, 0.90, 0.85, and 0.82 after 2, 6, 18, 24, 48, and 72 h, respectively) was demonstrated. In contrast, a negative correlation between 2-ME concentration and LH secretion was observed (r=-0.82, -0.72, -0.85, -0.89, -0.93, and -0.79 after 2, 6, 18, 24, 48, and 72 h, respectively). The concentration of O₂⁻ was increasing under the influence of the rising doses of 2-ME, whereas the mean level of MDA was not changing significantly.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

56

Numer

3

Opis fizyczny

p.373-378,fig.,ref.

Twórcy

  • Department of Pathophysiology, Chair of Preclinical Veterinary Science, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-033 Lublin, Poland
autor
autor

Bibliografia

  • 1. Banerjeei S.K., Zoubine M.N., Sarkar D.K., Weston A.P., Shah J.H., Campbell D.R.: 2-Methoxyestradiol blocks estrogen-induced rat pituitary tumor growth and tumor angiogenesis: possible role of vascular endothelial growth factor. Anticancer Res 2000, 20, 2641-2645.
  • 2. Basini G., Bussolati S., Santini S.E., Bianchi F., Careri M., Mangia A., Musci M., Grasselli F.: Antiangiogenesis in swine ovarian follicle: A potential role for 2- methoxyestradiol. Steroids 2007, 72, 660-665.
  • 3. Basini G., Santini S.E., Grasselli F.: 2-Methoxyestradiol inhibits Superoxide anion generation while it enhances superoxide dismutase activity in swine granulosa cells. Ann N Y Acad Sci 2006, 1091, 34-40.
  • 4. Bianchi F., Mattarozzi M., Careri M., Mangia A., Musci M., Grasselli F., Bussolati S., Basini G.: An SPME-GC- MS method using an octadecyl silica fibre for the determination of the potential angiogenesis modulators 17-ß estradiol and 2-methoxyestardiol in culture media. Anal Bioanal Chem 2010, 396, 2639-2645.
  • 5. Bogacka I., Siawrys G., Okrasa S., Kamiński T., Przała J.: The influence of GnRH, oxytocin and vasoactive intestinal peptide on the secretion of ß-endorphin and production of cAMP and cGMP by porcine pituitary cells in vitro. Anim Reprod Sci 2002, 69, 125-137.
  • 6. Bourghardt J., Bergstrom G., Krettek A., Sjoberg S., Boren J., Tivesten A.: The endogenous estradiol metabolite 2-methoxyestradiol reduces atherosclerotic lesion formation in female apolipoprotein E - deficient mice. Endocrinology 2007, 148, 4128-4132.
  • 7. Das U.N.: A radical approach to cancer. Med Sci Monit 2002, 8, 79-92.
  • 8. Fenoy F.J., Hernandez M.E., Hernandez M., Quesada T., Salom M.G.: Acute effects of 2-methoxyestradiol on endothelial aortic NO release in male and ovariectomized female rats. Nitric Oxide 2010, 23, 12-19.
  • 9. Hagen T., D'Amico G., Quintero M., Palacios-Callender M., Hollis V., Lam F., Moneada S.: Inhibition of mitochondrial respiration by anticancer agent 2-ME. Biochem Biophys Res Commun 2004, 322, 923-929.
  • 10. Huang P., Feng L., Oldham E.A., Keating M.J., Plunkett W.: Superoxide dismutase as a target for the selective killing of cancer cells. Nature 2000, 407, 390-395.
  • 11. Kosior-Korzecka U., Bobowiec R.: Effect of leptin, insulin and IGF-I on GnRH-induced LH secretion from porcine pituitary cells in vitro. Bull Vet Inst Pulawy 2007, 51, 439-443.
  • 12. Kosior-Korzecka U., Bobowiec R.: Leptin effect on nitric oxide and GnRH-induced FSH secretion from ovine pituitary cells in vitro. J Physiol Pharmacol 2006, 57, 637-647.
  • 13. LaVallee T.M., Zhan X.H., Herbstritt Ch.J., Kough E.C., Green S.J., Pribluda V.S.: 2-Methoxyestradiol inhibits proliferation and induces apoptosis independently of estrogen receptors α and ß. Cancer Res 2002, 62, 3691- 3697.
  • 14. Liu Z.J., Zhu B.T.: Concentration-dependent mitogenic and antiproliferative actions of 2-methoxyestradiol in estrogen receptor-positive human breast cancer cells. J Steroid Biochem 2004, 88, 265-275.
  • 15. Mueck A.O., Seeger H.: 2-Methoxyestradiol-biology and mechanism of action. Steroids 2010, 75, 625-631.
  • 16. Newman S.P., Foster P.A., Ho Y.T., Day J.M., Raobaikady B., Kasprzyk P.G., Leese M.P., Potter B.V.L., Reed M.J., Purohit A.: The therapeutic potential of a series of orally bioavailable antiangiogenic microtubule disruptors as therapy for hormone-independent prostate and breast cancers. Br J Cancer 2007, 97, 1673-1682.
  • 17. Pribluda V.S., Gubish E.R., LaVellee T.M., Treston A., Swartz G.M., Green S.J.: 2-Methoxyestradiol: an endogenous antiangiogenic and antiproliferative drug candidate. Cancer Metastasis Rev 2000, 19, 173-179.
  • 18. Ricker J.L., Chen Z., Yang X.P., Pribluda V.S., Swartz G.M., Van Waes C.: 2-Methoxyestradiol inhibits hypoxia-inducible factor la, tumor growth, and angiogenesis and augments paclitaxel efficacy in head and neck squamous cell carcinoma. Clin Cancer Res 2004, 10, 8665-8673.
  • 19. Shang W., Konidari I., Schomberg D.W.: 2- Methoxyestradiol, an endogenous estradiol metabolite, differentially inhibits granulosa and endothelial cell mitosis: a potential follicular antiangiogenic regulator. Biol Reprod 2001, 65, 622-627.
  • 20. Schumacher G., Neuhaus P.: The physiological estrogen metabolite 2-methoxyestradiol reduces tumor growth and induces apoptosis in human solid tumors. J Cancer Res Clin Oncol 2001, 127, 405-410.
  • 21. Spicer L.J., Walega M.A., Hammond J.M.: Metabolism of [³H]2-hydroxyestradiol by cultured porcine granulosa cells: Evidence for the presence of a catechol-O- methyltransferase pathway and a direct stimulatory effect of 2-methoxyestradiol on progesterone production. Biol Reprod 1987, 36, 562-571.
  • 22. Zacharia L.C., Jackson E.K., Gillespie D.G., Dubey R. K.: Increased 2-methoxyestradiol production in human coronary versus aortic vascular cells. Hypertension 2001, 37, 658-662.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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