Ameliorative effect of Caryopteris grata benth. against arsenic-induced enzymatic alterations in Testis of Albino Balb/c Mice

• Autorzy: Ullah K. , Jahan S. , Aziz F. , Khan M.S. , Rahman K.U. , Shah Z.A. , Rahman T.U.
• Języki publikacji: EN

Abstrakty

EN

Arsenic (As) contaminates drinking water worldwide and its potential impact on human health has been studied for decades. A number of traditional medicinal plants are used to counteract arsenic’s deleterious effects on humans. Phytochemical screening of Caryopteris grata revealed the presence of Quercetin, myricetin, sesquiterpenoids, and spathulenol. The present study was designed to evaluate whether the crude extract of leaves of C. grata inhibited arsenic-induced testicular toxicity in albino BALB/c mice. To examine the effects, mice were divided into five groups (A-E) of 6 mice each: Group A was provided with distilled water and served as a control. Group B mice were given sodium arsenite (NaAsO₂) at a dose of 10 mg/kg body weight (bw) for 10 days orally. Groups C, D, and E were treated with NaAsO₂ plus graded doses of C. grata extract (150, 300, 400 mg/kg bw), respectively. Testicular activities of antioxidant enzymes such as CAT, POD, SOD, GSH, GSR, and serum testosterone levels were significantly (P<0.05) decreased in Group B compared to Group A. C. grata markedly normalized (P<0.05) levels of CAT (0.72 and 0.46 U/mint), POD (0.311 and 0.126 U/mint), SOD (2.11 and 1.33 U/mg protein), GSH (0.679 and 0.284 µmol/g), and GSR (216.11 and 172.25 nmol/min/mg protein), along with testosterone levels (1.18±0.017 and 0.38±0.021 ng/ml) at the tested doses, respectively. Conclusively, the crude extract of C. grata leaves may inhibit both arsenic’s adverse effect on male reproductive hormones and testicular oxidative damage in mice.

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2019
• Tom: 28
• Numer: 2
• Opis fizyczny: p.861-866,fig.,ref.

Twórcy

autor

Ullah K.

• Department of Zoology, University of Swabi, Anbar, Khyber Pakhtunkhwa, Pakistan
• Laboratory of Reproductive Physiology, Department of Animal Sciences, Quaid-i-Azam University, Islamabad, Pakistan
• Department of Pathology and Pathophysiology, School of Medicine, Zhejiang University, Hangzhou, China

autor

Jahan S.

• Laboratory of Reproductive Physiology, Department of Animal Sciences, Quaid-i-Azam University, Islamabad, Pakistan

autor

Aziz F.

• Department of Zoology, University of Swabi, Anbar, Khyber Pakhtunkhwa, Pakistan

autor

Khan M.S.

• Department of Zoology, University of Swabi, Anbar, Khyber Pakhtunkhwa, Pakistan

autor

Rahman K.U.

• Department of Chemistry, University of Swabi, Anbar, Khyber Pakhtunkhwa, Pakistan

autor

Shah Z.A.

• Department of Chemistry, University of Swabi, Anbar, Khyber Pakhtunkhwa, Pakistan

autor

Rahman T.U.

• Department of Pathology and Pathophysiology, School of Medicine, Zhejiang University, Hangzhou, China

Bibliografia

• 1. DAVAR R., SEKHAVAT L., NASERZADEH N. Semen parameters of non-infertile smoker and non-smoker men. J. med life. 5 (4), 465, 2012.
• 2. AITKEN R.J., SMITH T.B., JOBLING M.S., BAKER M.A., DE IULIIS G.N. Oxidative stress and male reproductive health. Asian J. androl. 16 (1), 31, 2014.
• 3. PAUL S., CHAKRABORTY S., ALI M.N., RAY D.P. Arsenic distribution in environment and its bioremediation: A review. Int J. Agri Envi Biotechnol. 8 (1), 189, 2015.
• 4. JOMOVA K., JENISOVA Z., FESZTEROVA M., BAROS S., LISKA J., HUDECOVA D., VALKO M. Arsenic: toxicity, oxidative stress and human disease. J. App Toxicol. 31 (2), 95, 2011.
• 5. JOMOVA K., VALKO M. Advances in metal-induced oxidative stress and human disease. Toxicol. 283 (2), 65, 2011.
• 6. HANSEN J.M., ZHANG H., JONES D.P. Differential oxidation of thioredoxin-1, thioredoxin-2, and glutathione by metal Ions. Free Radic Biol. Med. 40, 138, 2006.
• 7. EL-DEMERDASH F.M., YOUSEF M.I., RADWAN F.M. Ameliorating effect of curcumin on sodium arsenite-induced oxidative damage and lipid per oxidation in different rat organs. Food Chem Toxicol. 47 (1), 249, 2009.
• 8. AGARWAL A., VIRK G., ONG C., DU PLESSIS S.S. Effect of oxidative stress on male reproduction. World J Mens Health. 32 (1), 1, 2014.
• 9. POWERS S.K., JACKSON M.J. Exercise-induced oxidative stress: cellular mechanisms and impact on muscle force production. Physiol rev. 88 (4), 1243, 2008.
• 10. CHABORY E., DAMON C., LENOIR A., KAUSELMANN G., KERN H., ZEVNIK B. Epididymis seleno-independent glutathione peroxidase 5 maintains sperm DNA integrity in mice. J. Clin. Invest. 119 (7), 2074, 2009.
• 11. AITKEN R., DE IULIIS G. On the possible origins of DNA damage in human spermatozoa. Molecular human reproduction. Basic Sci. Reprod. Med. 16, 3, 2010.
• 12. GHARAGOZLOO P., AITKEN R.J. The role of sperm oxidative stress in male infertility and the significance of oral antioxidant therapy. Human. Reprod. 26 (7), 1628, 2011.
• 13. DUA T.K., DEWANJEE S., GANGOPADHYAY M., KHANRA R., ZIA-UL-HAQ M., DE FEO V. Ameliorative effect of water spinach, Ipomea aquatica (Convolvulaceae), against experimentally induced arsenic toxicity. J. Transl. Med.13 (1), 81, 2015.
• 14. MAZUMDER D.G. Health Effects Chronic Arsenic Toxicity. Handbook. Arsenic Toxicol. 137, 2015.
• 15. MATHUR N., PANDEY G., JAIN G.C. Male reproductive toxicity of some selected metals: A review. J. Biol Sci. 10 (5), 396, 2010.
• 16. WU Z.Q., CHEN D.L., LIN F.H., LIN L., SHUAI O., QI L.K., ZHANG P. Effect of bajijiasu isolated from Morinda officinalis FC how on sexual function in male mice and its antioxidant protection of human sperm. J. Ethnopharmacol. 164, 283, 2015.
• 17. UPADHYAYA K., DIXIT V., PADALIA R., MATHELA C. Chemical composition of the essential oil of Caryopteris grata Benth. J. Essent Oil Res. 21 (1), 69, 2009.
• 18. JAHAN S., IFTIKHAR N., ULLAH H., RUKH G., HUSSAIN I. Alleviative effect of quercetin on rat testis against arsenic: a histological and biochemical study. Sys Bio. Reprod Med. 61 (2), 89, 2015.
• 19. WILMS L.C., HOLLMAN P.C., BOOTS A.W., KLEINJANS J.C. Protection by quercetin and quercetin-rich fruit juice against induction of oxidative DNA damage and formation of BPDE-DNA adducts in human lymphocytes. Mutat Res/Genet Toxicol. Env Mutat. 582 (1), 155, 2005.
• 20. SHAH N.A., KHAN M.R., NADHMAN A. Antileishmanial, Toxicity, and Phytochemical Evaluation of Medicinal Plants Collected from Pakistan. Biomed. Res. Int. 2014; 384204-384207 doi: 10.1155/2014/384204.
• 21. TADESSE W.T., HAILU A.E., GURMU A.E., MECHESSO A.F. Experimental assessment of antidiarrheal and antisecretory activity of 80% methanolic leafextract of Zehneria scabra in mice. BMC Complement. Altern. Med. 14 (1), 460, 2014.
• 22. CHANCE B., MAEHLY A. Assay of catalases and peroxidases. Methods. Enzymol. 2, 764, 1995.
• 23. KAKKAR P., DAS B., VISWANATHAN P. A modified spectrophotometric assay of superoxide dismutase. Indian J. Biochem. Biophys. 21, 130, 1948.
• 24. JOLLOW D., MITCHELL J., ZAMPAGLIONE N.A., GILLETTE J. Bromobenzene-induced liver necrosis. Protective role of glutathione and evidence for 3, 4-bromobenzene oxide as the hepatotoxic metabolite. Pharmacol. 11 (3), 151, 1974.
• 25. CARLBERG I., MANNERVIK E.B. Glutathione level in rat brain. J. Biol. Chem. 250, 4475, 1975.
• 26. TREMELLEN K. Oxidative stress and male infertility - a clinical perspective. Human Reprod. Update. 14 (3), 243, 2008.
• 27. KOPPERS A.J., DE IULIIS G.N., FINNIE J.M., MCLAUGHLIN E.A., AITKEN R.J. Significance of mitochondrial reactive oxygen species in the generation of oxidative stress in spermatozoa. J. Clin. Endocrinol. Metab. 93 (8), 3199, 2008.
• 28. AITKEN R.J., DE IULIIS G.N., MCLACHLAN R.I. Biological and clinical significance of DNA damage in the male germ line. Int. J. Androl. 32 (1), 46, 2009.
• 29. ZHANG W., XUE J., GE M., YU M., LIU L., ZHANG Z. Resveratrol attenuates hepatotoxicity of rats exposed to arsenic trioxide. Food Chem. Toxicol. 51, 87, 2013.
• 30. DAS A.K., BAG S., SAHU R., DUA T.K., SINHA M.K., GANGOPADHYAY M. Protective effect of Corchorus olitorius leaves on sodium arsenite-induced toxicity in experimental rats. Food Chem. Toxicol. 48 (1), 326, 2010.
• 31. GHOSH P., BISWAS N., GHOSH D. Effect of lithium chloride on testicular steroidogenesis and gametogenesis in immature male rats. Acta. endocrinol. 124 (1), 76, 1991.
• 32. ADIL M., VISNAGRI A., KUMAR V. S., KANDHARE A. D., GHOSH P., BODHANKAR S. L. Protective effect of naringin on sodium arsenite induced testicular toxicity via modulation of biochemical perturbations in experimental rats. Pharmacol. 5 (222), 234, 2014.
• 33. EBRAHIM M., POOL E.J. The effect of Tulbaghia violacea extracts on testosterone secretion by testicular cell cultures. J. Ethnopharmacol. 132 (1), 359, 2010.
• 34. DESHMUKH S.A., GAIKWAD D.K. A review of the taxonomy, ethnobotany, phytochemistry and pharmacology of Basella alba (Basellaceae). 2013.
• 35. CHATTOPADHYAY S., MAITI S., MAJI G., DEB B., PAN B., GHOSH D. Protective role of Moringa oleifera (Sajina) seed on arsenic-induced hepatocellular degeneration in female albino rats. Biol. Trace. Elem. Res. 142 (2), 200, 2011.

Identyfikatory

DOI

10.15244/pjoes/84831