The impact of local tissue microenvironment on the oligodendrocyte progenitor differentiation

• Autorzy: Sypecka J. , Sarnowska A. , Winiarska H. , Domanska-Janik K. , Lukomska B.
• Języki publikacji: EN

Abstrakty

EN

The oligodendrocyte precursors exhibit many features of neural stem cells and constitute the abundant population of dividing progenitors in the young and adult brain. A question arises if their commitment and development could be modulated by either local tissue-specific or neuropathological signals. The aim of our study was to evaluate the effect of distinct microenvironments (provided by either the spinal cord or the hippocampal slices) on the differentiation of rat neonatal NG2 cells. The hippocampal slice cultures subjected to an ischemic injury (OGD) were used to mimic the traumatized tissue microenvironment. Both the hippocampal and spinal cord slice cultures were established from the same 7-day old rats. The model of an indirect contact (i.e. exclusively by the culture media) in co-culture system was chosen to eliminate the influence of cell-cell contact. The NG2 cells were obtained from 10-day old mixed primary culture of neonatal rat hemispheres. After 7 days in co-culture, the cells were either stained with neural markers or collected for the RNA isolation and real-time PCR. The medium conditioned by hippocampal slices effectively promoted neurogenesis: ~30% of NG2 cells differentiated into TUJ 1-positive neurons. The remaining fraction mostly formed premyelinating and mature oligodendrocytes. The exposition of hippocampal slices to the OGD injury abolished the effect of pro-neuronal induction in cocultures. In media conditioned by spinal cord slices, neurogenesis was less pronounced (20% neurons) and the oligodendrocyte differentiation was significantly slowed-down. The NG2 precursors have the intrinsic potency for neurogenesis. Heterogeneity of local microenviroment might modify the fate of endogenous or transplanted NG2 cells what should be taken into consideration in potential neurorepair strategies. Supported by grant 0345/B/P01/2010/38.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2011
• Tom: 71
• Numer: S
• Opis fizyczny: p.41

Twórcy

autor

Sypecka J.

• NeuroRepair Department, Mossakowski Medical Research Centre PAS, Warsaw, Poland

autor

Sarnowska A.

• NeuroRepair Department, Mossakowski Medical Research Centre PAS, Warsaw, Poland

autor

Winiarska H.

• NeuroRepair Department, Mossakowski Medical Research Centre PAS, Warsaw, Poland

autor

Domanska-Janik K.

• NeuroRepair Department, Mossakowski Medical Research Centre PAS, Warsaw, Poland

autor

Lukomska B.

• NeuroRepair Department, Mossakowski Medical Research Centre PAS, Warsaw, Poland

Uwagi

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