Efficacy of Candida melibiosica for control of post-harvest fungal diseases of carrot (Daucus carota L.)

• Autorzy: Kordowska-Wiater M. , Wagner A. , Hetman B.

Warianty tytułu

PL

Skuteczność Candida melibiosica w kontroli pozbiorowych chorób grzybowych marchwi (Daucus carota L.)

• Języki publikacji: EN

Abstrakty

EN

Biological control using antagonistic yeast has emerged as one of the most promising alternatives in postharvest protection of fruit and vegetables. The aim of the present studies was to investigate the potential of the yeast Candida melibiosica for biocontrol of Botrytis cinerea, Alternaria alternata and A. radicina, fungi pathogenic to carrot roots, and explain the possible mode of action of this antagonistic microorganism. The ability of the yeast to inhibit fungal growth in vitro, produce volatile compounds and lytic enzymes, and protect infected carrot roots was studied. C. melibiosica inhibited the growth of B. cinerea on potato-dextrose agar and malt agar by 25.9% and 33.3%, respectively, but only slightly restricted the growth of A. radicina and had no effect on A. alternata. The volatile compounds acting against B. cinerea and A. radicina were produced by C. melibiosica growing on malt agar. The yeast produced E-glucanase and chitinase with activities between 34.0–232.86 U · cm⁻³ and 20.74–43.7 U · cm⁻³, respectively. Application of the yeast to carrot roots before inoculation with the fungi B. cinerea and A. radicina reduced the progress of the disease.

PL

Kontrola biologiczna z użyciem antagonistycznych drożdży jest jedną z najbardziej obiecujących metod ochrony pozbiorowej owoców i warzyw. Celem badań była ocena potencjału drożdży Candida melibiosica do biokontroli wzrostu Botrytis cinerea, Alternaria alternata i A. radicina, grzybów chorobotwórczych dla korzeni marchwi i wyjaśnienie możliwego sposobu działania tego antagonistycznego mikroorganizmu. Badano zdolność drożdży do hamowania wzrostu grzybów in vitro, produkcji związków lotnych i enzymów litycznych (β-glukanazy i chitynazy) oraz ochrony zakażonych korzeni marchwi. Gatunek C. melibiosica ograniczał wzrost B. cinerea na podłożu PDA o 25,9%, a na agarze brzeczkowym o 33,3%, natomiast słabo ograniczał wzrost A. radicina i w ogóle nie oddziaływał na A. alternata. Drożdże na agarze brzeczkowym produkowały związki lotne działające przeciwko B. cinerea i A. radicina. Wytwarzały β-glukanazę i chitynazę z aktywnościami wynoszącymi odpowiednio 34,0–232,86 U · cm⁻³ i 20,74–43,7 U · cm⁻³. Zastosowanie drożdży na korzeniach marchwi przed inokulacją grzybami zredukowało na nich rozwój choroby podczas przechowywania.

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Hortorum Cultus
• Rocznik: 2012
• Tom: 11
• Numer: 5
• Opis fizyczny: p.55-65,fig.,ef.

Twórcy

autor

Kordowska-Wiater M.

• Department of Biotechnology, Human Nutrition and Science of Food Commodities, University of Life Sciences in Lublin, Skromna 8, 20-704 Lublin, Poland

autor

Wagner A.

• University of Life Sciences in Lublin, Lublin, Poland

autor

Hetman B.

• University of Life Sciences in Lublin, Lublin, Poland

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