Inhibitory effect of epstein-barr virus gene-ebnal1 on human TNFRp55 gene expression

• Autorzy: Krzowska-Firych J. , Fota-Markowska H. , Marzec-Kotarska B. , Modrzewska R. , Wojcierowski J.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was to assess the expression of TNFRp55 mRNA and to examine if the antisense inhibition of Epstein-Barr virus (EBV) encoded EBNA1 gene product alters the expression of gene encoding TNFRp55 in lymphoblastoid cell line (LCL). The experiment was performed on LCL derived from EBV infected human peripheral blood B lymphocytes. The lymphocytes were isolated and cultured. RNA was isolated and examined according to the RNase protection assay. The hybridisation was done with HCR-4 probe. RNA was quantified by densitometry and presented in extinction units. The level of expression was calculated with TotalLab software programme. The results of the study suggest that EBV gene, responsible for the synthesis of EBNA1 protein, has an inhibitory effect on human TNFRp55 gene expression in LCL.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2012
• Tom: 56
• Numer: 3
• Opis fizyczny: p.271-274,fig.,ref.

Twórcy

autor

Krzowska-Firych J.

• Department of Infecious Diseases, Medical University of Lublin, 20-081 Lublin, Poland

autor

Fota-Markowska H.

autor

Marzec-Kotarska B.

autor

Modrzewska R.

autor

Wojcierowski J.

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