Antimicrobial susceptibility of Brachyspira hyodysenteriae isolated from 21 Polish farms

• Autorzy: Zmudzki J. , Szczotka A. , Nowak A. , Strzelecka H. , Grzesiak A. , Pejsak Z.
• Języki publikacji: EN

Abstrakty

EN

Swine dysentery (SD) is a common disease among pigs worldwide, which contributes to major production losses. Antimicrobial susceptibility testing of B. hyodysenteriae, the etiological agent of SD, is mainly performed by the agar dilution method. This method has certain limitations due to difficulties in interpretation of results. The aim of this study was the analysis of antimicrobial susceptibility of Brachyspira hyodysenteriae (B. hyodysenteriae) Polish field isolates by broth microdilution procedure. The study was performed on 21 isolates of B. hyodysenteriae, collected between January 2006 to December 2010 from cases of swine dysentery. VetMIC™ Brachyspira panels with antimicrobial agents (tiamulin, valnemulin, doxycycline, lincomycin, tylosin and ampicillin) were used for susceptibility testing of B. hyodysenteriae. The minimal inhibitory concentration (MIC) was determined by the broth dilution procedure. The lowest antimicrobial activity was demonstrated for tylosin and lincomycin, with inhibition of bacterial growth using concentrations >128 μg/ml and 32 μg/ml, respectively. In the case of doxycycline, the MIC values were ≤ 2.0 μg/ml. No decreased susceptibility to tiamulin was found among the Polish isolates and MIC values for this antibiotic did not exceed 1.0 μg/ml. The results of the present study confirmed that Polish B. hyodysenteriae isolates were susceptible to the main antibiotics (tiamulin and valnemulin) used in treatment of swine dysentery. Further studies are necessary to evaluate a possible slow decrease in susceptibility to tiamulin and valnemulin of B. hyodysenteriae strains in Poland.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2012
• Tom: 15
• Numer: 2
• Opis fizyczny: p.259-265,fig.,ref.

Twórcy

autor

Zmudzki J.

• National Veterinary Research Institute, Swine Diseases Department, Partyzantow 57, 24-100 Pulawy, Poland

autor

Szczotka A.

autor

Nowak A.

autor

Strzelecka H.

autor

Grzesiak A.

autor

Pejsak Z.

Bibliografia

• Belanger M, Jacques M (1991) Evaluation of the An-Ident System and an indole spot test for the rapid differentiation of porcine treponemes. J Clin Microbiol 8: 1727-1729.
• Binek M, Szynkiewicz ZM (1984) Physiological properties and classification of strains of Treponema sp. Isolated from pigs in Poland. Comp Immunol Microbiol Infect Dis 7: 141-148.
• Binek M, Szynkiewicz Z, Spohr de Faundez I, Wójcik U, Klimuszko D, Rzewuska M (1995) The cytotoxic properties of Serpulina hyodysenteriae. Acta Microbiol Pol 44: 63-68.
• Binek M, Wojcik U, Szynkiewicz Z, Jakubowski T, Poomvises P, Ingkaninun P (1994) Dynamics of susceptibility of Serpulina hyodysenteriae to different chemotherapeutics in vitro. Proc Inter Pig Vet Soc Congr, Bangkok, Thailand, p 203.
• Clinical and Laboratory Standards Institute (2007) Methods for Antimicrobial Susceptibility Testing of Anaerobic Bacteria; Approved Standard-Seventh Edition, M11-A7; link: http://www.clsi.org/source/orders/free/m11a7.pdf
• European Committee on Antimicrobial Susceptibility Testing (2012) Clinical breakpoints-bacteria, Version 2.0, valid from 2012-01-01; link: http://www.eucast.org/fileadmin/ src/media/PDFs/EUCAST–files/Breakpoint–tables/Breakpoint–table–v–2.0–120221.pdf
• Fossi M, Saranpaa T, Rautiainen E (1999) In vitro sensitivity of the swine Brachyspira species to tiamulin in Finland, 1995-1997. Acta Vet Scand 40: 355-358.
• Gresham AC, Hunt BW, Dalziel RW (1998) Treatment of swine dysentery – problems of antibiotic resistance and concurrent salmonellosis. Vet Rec 143: 619.
• Hampson D (2008) Detection and strain typing of Brachyspira hyodysenteriae to support swine dysentery eradication and control. Report prepared for the Co-operative Research Centre for an Internationally Competitive Pork Industry; link: http://www.porkcrc.com.au/2C-102–Final–Project–Report.pdf
• Hidalgo A, Carvajal A, García-Feliz C, Osorio J, Rubio P (2009) Antimicrobial susceptibility testing of Spanish field isolates of Brachyspira hyodysenteriae. Res Vet Sci 87: 7-12.
• Kärlsson M, Fellström C, Gunnarsson A, Landen A, Franklin A (2003) Antimicrobial susceptibility testing of porcine Brachyspira (Serpulina) species isolates. J Clin Microbiol 41: 2596-2604.
• Kärlsson M, Fellström C, Heldtander MU, Johansson KE, Franklin A (1999) Genetic basis of macrolide and lincosamide resistance in Brachyspira (Serpulina) hyodysenteriae. FEMS Microbiol Lett 172: 255-260.
• Kärlsson M, Gunnarsson A, Franklin A (2001) Susceptibility to pleuromutilins in Brachyspira (Serpulina) hyodysenteriae. Anim Health Res Rev 2: 59-65.
• Kärlsson M, Oxberry SL, Hampson DJ (2002) Antimicrobial susceptibility testing of Australian isolates of Brachyspira hyodysenteriae using a new broth dilution method. Vet Microbiol 84: 123-133.
• Lobova D, Smola J, Cizek A (2004) Decreased susceptibility to tiamulin and valnemulin among Czech isolates of Brachyspira hyodysenteriae. J Med Microbiol 53: 287-291.
• Molnar L (1996) Sensitivity of strains of Serpulina hyodysenteriae isolated in Hungary to chemotherapeutic drugs. Vet Rec 138: 158-160.
• Pejsak Z, Żmudzki J, Wasak M, Porowski M, Wojciechowski J, Mirt D (2007) Prevalence of Brachyspira hyodysenteriae and Lawsonia intracellularis infections in Polish swine population. Med Weter 63: 994-996.
• Pringle M, Aarestrup FM, Bergsjo B, Fossi M, Jouy E, Landen A, Mevius D, Perry K, Teale C, Thomson J, Skrzypczak T, Veldman K, Franklin A (2006) Quality-control ranges for antimicrobial susceptibility testing by broth dilution of the Brachyspira hyodysenteriae type strain (ATCC 27164T). Microb Drug Resist 12: 219-221.
• Pringle M, Fellström C, Johansson KE (2007) Decreased susceptibility to doxycycline associated with a 16S rRNA gene mutation in Brachyspira hyodysenteriae. Vet Microbiol 123: 245-248.
• Räsback T, Fellström C, Bergsjo B, Cizek A, Collin K, Gunnarsson A, Jensen SM, Mars A, Thomson J, Vyt P, Pringle M (2005) Assessment of diagnostics and antimicrobial susceptibility testing of Brachyspira species using a ring test. Vet Microbiol 109: 229-243.
• Rohde J, Kessler M, Baums CG, Amtsberg G (2004) Comparison of methods for antimicrobial susceptibility testing and MIC values for pleuromutilin drugs for Brachyspira hyodysenteriae isolated in Germany. Vet Microbiol 102: 25-32.
• Rønne H, Szancer J (1990) In vitro susceptibility of Danish field isolates of Treponema hyodysenteriae to chemotherapeutics in swine dysentery (SD) therapy. Proc Inter Pig Vet Soc Congr, Lausanne, Switzerland, p 126.
• Szynkiewicz ZM, Binek M (1986) Evaluation of selective media for primary isolation of Treponema hyodysenteriae and Treponema innocens. Comp Immunol Microbiol Infect Dis 9: 71-77.
• Uezato K, Kinjo E, Adachi Y (2004) In vitro susceptibility of 21 antimicrobial agents to 37 isolates of Brachyspira hyodysenteriae isolated from pigs in Okinawa Prefecture. J Vet Med Sci 66: 307-309

Uwagi

Rekord w opracowaniu