Effect of muscarinic receptors activation on membrane potential in medial prefrontal cortex (mPFC) pyramidal neurons

• Autorzy: Kurowski P.M. , Szulczyk P.
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to clarify the mechanisms involved in regulation of membrane potential in mPFC pyramidal neurons by muscarinic receptors. Recordings of membrane potential were performed with the gramicidin perforated-patch method on mPFC pyramidal neurons in slices obtained from 20-day-old rats. It was determined that muscarinic receptor stimulation by carbachol (100 µM) evoked membrane potential depolarization (10.0±1.3 mV), which was completely eliminated by the M1 receptor antagonist pirenzepine (2 µM), by removing Na+ ions from the extracellular solution, in the presence of a TTX-resistant Na+ channel blocker amitriptyline (100 µM) and significantly reduced by the inhibitor of G-protein βγ-subunits gallein (20 µM). We conclude that muscarinic M1 receptor-dependent depolarisation in mPFC pyramidal neurons is evoked by activation of TTX-resistance Na+ currents by G-protein βγ-subunits. Supported by grants no: NN401584638 and NN301572940

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2014
• Tom: 74
• Numer: 3
• Opis fizyczny: p.333

Twórcy

autor

Kurowski P.M.

• Department of Physiology and Pathophysiology, Medical University of Warsaw, Warsaw, Poland

autor

Szulczyk P.

• Department of Physiology and Pathophysiology, Medical University of Warsaw, Warsaw, Poland